Table of Content

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• Current Biotechnology
  Volume 5 Issue 3 25 May 2015
  Special Issue on Marine Biotechnology
• Review of Algae Biomass Resources Conversion
• QIU Qing\|qing, REN Xiu\|lian, WU Ze, CHEN Yong\|xing, WEI Qi\|feng*
• 2015, 5(3):  153-157.  DOI: 10.3969/j.issn.20952341.2015.03.01
• Abstract ( ) PDF (1111KB)( )
With the global fossil energy are depleted, seeking renewable biomass sources is imperative, therefore algae biomass resources are increasingly concerned about. This article described the research of algae biomass enegy conversion which was reported, including the development of biodiesel fuel technologies for alternative, and the use of algae biomass resources conversion for high value\|added chemicals, which was expected to provide reference for development and utilization study of algae biomass resources.
• Progress and Prospect of Seaweed Fertilizer
• WANG Ming-peng1, CHEN Lei2, LIU Zheng-yi2, QIN Song2*, YAN Pei-sheng1*
• 2015, 5(3):  158-163.  DOI: 10.3969/j.issn.20952341.2015.03.02
• Abstract ( ) PDF (1125KB)( )
Seaweed and seaweed\|derived products have been widely used in organic fertilizer manufacturing and crop production systems due to the presence of a number of plant growth\|stimulating compounds. Compared with chemical fertilizer, seaweed fertilizer is nontoxic, high effective and environmental friendly. Hence, seaweed fertilizer has become a popular product in fertilizer field. Studies on interactions of seaweed fertilizer with the crop and the soil community also become the focus of academic research. This article provided a comprehensive review of research progress in seaweed fertilizer manufacturing industry including materials, technology, active components and effects, which was expected to indicate new ideas and directions for seaweed fertilizer industry developing.
• Advance on Marine Petroleum Pollution and Microbial Remediation
• LI Gui-zhen1,2, LAI Qi-liang2, YAN Pei-sheng1,3*, SHAO Zong-ze1,2*
• 2015, 5(3):  164-169.  DOI: 10.3969/j.issn.20952341.2015.03.03
• Abstract ( ) PDF (1135KB)( )
Marine petroleum pollution have a serious effect on the marine ecosystems and human health. Microbial remediation technology for marine petroleum pollution is attracting extensive attention for its advantages. This paper introduced the current situation of marine petroleum pollution, the treatments of marine petroleum pollution, and microbial remediation of marine petroleum pollution. The paper mainly focused on the diversity of petroleum degrading microorganisms, mechanism of degradation and the advance of boiremediation. Meanwhile, this paper also pointed out the problems of microbial remediation which need to be overcomed, and hoped to provide useful informations for the study on marine environmental microbial remediation of petroleum pollution.
• Progress on Diversity and Secondary Metabolites Biological Activity of Deep-sea Fungi
• LI Meng-ying, YAN Pei-sheng*, GAO Xiu-jun, SUN Xiao-lei
• 2015, 5(3):  170-175.  DOI: 10.3969/j.issn.2095-2341.2015.03.04
• Abstract ( ) PDF (1131KB)( )
The environments of deep-sea are complex and diverse，deep-sea microorganisms gradually evolved to adapt to the environment. Fungi is important microorganisms in deep-sea environment, which would be a new kind of resource for marine organisms exploitation. This paper summarized the diversities of species and bioactive metabolites, and their biological function on deep-sea fungi in recent years. The future application of deep-sea fungi was prospected.
• Progress of  Deep-sea Bacteria and its Bioactive Compounds on Prevention of Plant Pathogenic Fungi
• SUN Xiao-lei, YAN Pei-sheng*, WANG Kai, WANG Wen-wei, LI Meng-ying
• 2015, 5(3):  176-184.  DOI: 10.3969/j.issn.2095-2341.2015.03.05
• Abstract ( ) PDF (2016KB)( )
In recent years，the development of biopesticides from natural products has been concerned by scholars. For the unique living environment，marine microorganisms producing many novel bioactive compounds that never been found in the terrestrial microorganisms，which provide bright prospects for developing novel biopesticides. The research progress of deep-sea bacteria against phytopathogenic fungi was briefly summarized from three aspects of species diversity，kinds and structures of antifungal substances and antifungal mechanisms. Developing biological pesticide from marine microorganisms was prospected in this paper. This review might provide some insight for development and utilization of marine microorganisms in anti-phytopathogenic fungi.
• Advances on Enzymes from Marine Microorganisms
• SHI Cui-juan, YAN Pei-sheng*, ZHAO Rui-xi, HAN Xiao
• 2015, 5(3):  185-190.  DOI: 10.3969/j.issn.2095-2341.2015.03.06
• Abstract ( ) PDF (1147KB)( )
The microorganism is rich in ocean, and marine microorganism is becoming the important source of enzyme. Many species of marine bacteria, actinomycetes and fungi can produce enzyme with activity. The polysaccharase, lipolytic enzyme, protease, laccase and other enzymes from marine microorganisms were summarized, which was expected to provide reference for utilization study of marine microorganism.
• Research Progress on Cellulase Producing by Marine Microorganisms and its Application
• HAN Xiao, YAN Pei-sheng*, SHI Cui-juan, YANG Shu-yan, LI Yong-peng, ZOU Xue-ping
• 2015, 5(3):  191-195.  DOI: 10.3969/j.issn.2095-2341.2015.03.07
• Abstract ( ) PDF (1096KB)( )
Cellulose is the most ancient，abundant and inexhaustible natural polymer resources. Cellulase widely exists in the organism including bacteria，fungi and animals. Microbial cellulase has been widely reported and utilized in industries of food， medicine， feed， detergent， textile， pulp and paper，etc.. The ocean is a huge pool of resources，and cellulose-producing marine microorganisms have attracted a lot of attention. This paper briefly summarized the research progress of populations and source of marine microorganisms producing cellulase and genetic screening，the enzymatic properties and application fields of cellulase produced by marine microorganisms. Prospects of study on marine microbial cellulase were also discussed.
• Progress on Thioredoxin
• WANG Quan-fu, MIAO Miao, HOU Yan-hua*, SHI Yong-lei, HAN Han, WU Ying-ying, YAN Tian-qi, Qu Jun-jie
• 2015, 5(3):  196-200.  DOI: 10.3969/j.issn.2095-2341.2015.03.08
• Abstract ( ) PDF (1540KB)( )
Thioredoxin (Trx) is a kind of small-molecule multifunctional protein which exists widely in eukaryotic and prokaryotic organisms. It has functions of the regulation of cell growth, the inhibition of cell apoptosis and the regulation of gene transcription. Trx composes the thioredoxin system which plays an important role in maintaining redox-active stability in body with thioredoxin reductase (TrxR) and nicotinamide adenine nucleoside two phosphate (NADPH). This paper summarized the structural features, classification, distribution, biological and activity of Trx, which was expected to provide reference for related research.
• Research Progress of the Extraction Procedures and Bioactivities of  Brown Seaweed Polysaccharide
• WU Ze, WEI Qi-feng, QIU Qing-qing, REN Xiu-lian*
• 2015, 5(3):  201-206.  DOI: 10.3969/j.issn.2095-2341.2015.03.09
• Abstract ( ) PDF (1143KB)( )
Brown seaweeds polysaccharide usually contains alginate, fucoidan, laminam and mainly exist in brown seaweeds. As the main physiological active substances, fucoidan composes of L-fucose and sulfate groups. Fucoidan has different beneficial biological activities including antioxidant, anticoagulant, anti-cancer, anti-tumor, anti-virus and anti-inflammatory, etc. This paper reviewed the latest research progress of the extraction procedures and bioactivities of fucoidan, which was expected to provided reference for fucoidan application.
• Study on Construction of Benthonic Microbial Fuel Cells and its Influential Factors
• QI Zhen-lian, JIA Yu-hong*, MA Shan-shan, YOU Hong*
• 2015, 5(3):  207-212.  DOI: 10.3969/j.issn.2095-2341.2015.03.10
• Abstract ( ) PDF (1521KB)( )
Benthonic microbial fuel cells, with advantages of abundant sources in substrates, long-term operation, low maintenance cost and environmentally friendliness, etc, have high research value and broad prospects for development. At present, there are only limited usages for benthonic microbial fuel cells in practical application, mainly for its low power density output and long-term running instability. Therefore, this study took submarine sedimentary mud as the substrate for benthonic microbial fuel cells construction, and the cells were tested by different anode material, cathode-anode electrode area ratios, different anode modifications methods and anode depth underneath submarine sedimentary. Results showed that carbon felt was so far the best benthonic microbial fuel cell anode material, while the best cathode-anode electrode area ratio was 1∶1, the best anode modification method was ammonia treatment, and the best anode depth underneath submarine was 2 cm.
• Inhibition Effect of Kelp Residue Microbial Fertilizer on Mycotoxin-producing Fungus Aspergillus parasiticus in Soil
• XIAO Wei, YAN Pei-sheng*
• 2015, 5(3):  213-217.  DOI: 10.3969/j.issn.2095-2341.2015.03.11
• Abstract ( ) PDF (1093KB)( )
The inhibition effect of kelp residue microbial fertilizer on mycotoxin-producing fungus Aspergillus parasiticus was studied. The kelp residue microbial fertilizer was fermented with bio-control Bacillus Hitwh-BA2. Results showed that kelp residue microbial fertilizer have extremely significantly reduced the number of A. parasiticus and A. parasiticus percentage of total number of fungi, and its effect was better than kelp residue; kelp residue microbial fertilizer have extremely significantly improved the number of bacteria in cultivated soil, and the effect of kelp reside microbial fertilizer and kelp residue were extremely significant difference. The results suggestted that kelp residue microbial fertilizer could be used in biocontrol of aflatoxin-producing fungi in soils.
• Production of Microbial Flocculants from Sludge Extract
• WANG Shuang1, LI Ang2, LI Lin2, LI Wei-guo1*, MA Fang2*
• 2015, 5(3):  218-222.  DOI: 10.3969/j.issn.2095-2341.2015.03.12
• Abstract ( ) PDF (1472KB)( )
Use sewage sludge as media materials, process the sewage sludge through a series of conditions aiming to use the treated sewage sludge as the fermentation medium of microbial flocculant producing bacteria. The results showed that alkaline hydrolysis of the sludge at pH 12 was the optimal pretreatment conditions, with maximum output of  carbon and nitrogen. Sterilizing the sludge extract after adding 8 g/L glucose, the microbial flocculant producing bacteria LLin6 could normally produce flocculants, the flocculation rate could reach 91.55%. The present study would provide basis for  reducing the expected cost of bioflocculant preparation and realizing sludge reduction and resources reutilization.
• Bioconversion of Shrimp Processing Wastes to Antioxidant Exopolysaccharides by Cantharellus cibarius
• GAO Xiu-jun, YAN Pei-sheng*, LIU Xin, BAO Zheng, ZHU Yan-ping
• 2015, 5(3):  223-234.  DOI: 10.3969/j.issn.2095-2341.2015.03.13
• Abstract ( ) PDF (4380KB)( )
Shrimp processing waste was converted and deodorized for production of antioxidant polysaccharides by Cantharellus cibarius using response surface methodology. The optimal conditions for highest mycelium biomass and exopolysaccharides production were determined as follows: the solid to liquid ratio was 9.016%,  volume of liquid medium was 120.292 mL, and 1.208% concentration of glacial acetic acid. Under the optimal conditions, mycelium biomass and exopolysaccharides production reached 28.407±0.842 g/L and 7.009±0.517 g/L, respectively in validation test, which were consistent with the predicted values (RSD <5%). These values were improved by 60.564% and 114.737%, respectively compared to the initial culture conditions. The scores of deodorization were 3.056±0.115 and 3.694±0.076 at based and optimized validation bioconversion. The resulting exopolysaccharides had high antioxidant activities with total antioxidant capacity of 1.952±0.041 U/mg，superoxide anion scavenging capacity of 93.223±1.174 U/g and hydroxyl radical scavenging capacity of 60.291±0.638 U/mg. The study provided an alternative method for production of antioxidant polysaccharides from shrimp processing waste by Cantharellus cibarius.
• Isolation and Identification of a Microbacterium Strain from Deep-sea with Inhibition Activity Against Aflatoxin
• XU Jing-jing1, YAN Pei-sheng1*, WANG Kai1, MA Rui1, JIA Wen-wen1, DUNLAP Christopher A2
• 2015, 5(3):  235-239.  DOI: 10.3969/j.issn.2095-2341.2015.03.14
• Abstract ( ) PDF (1624KB)( )
An actinobacterial strain R104 was isolated from deep-sea water of the South Atlantic. After fermentation，the cell-free supernatant of strain R104 showed strong inhibition activity against aflatoxin producing with an inhibition ratio of 96.2%. This strain was identified as Microbacterium by 16S rDNA sequence analysis. To the best of our knowledge，this was the first report that Microbacterium from deep-sea was capable of inhibiting aflatoxin production.
• Preliminary Study on Salt Tolerance of Sea-ice Microorganism Pseudoa-lteromonas sp. ANT319
• WANG Quan-fu§, LIN Yi-fei§, MIAO Miao, SHI Yong-lei, HAN Han, WU Ying-ying, YANG Yang, HOU Yan-hua*
• 2015, 5(3):  240-245.  DOI: 10.3969/j.issn.2095-2341.2015.03.15
• Abstract ( ) PDF (1494KB)( )
Over long periods of evolution, Antarctic sea-ice microorganisms obtained special physiological and biochemical characterristics and gene expression regulation mechanisms to adapt extreme environment. To learn more about the adaption mechanism of the Antarctic microorganisms, the strains growth, total protein content, permeability of cell membrane, malondialdehyde and antioxidant enzymes system activity including SOD, POD and CAT had been studied under different salinity conditions of 3%~9% based on the Pseudoalteromonas sp. ANT319, which belongs to the typical genus in Antarctic bacteria. The results showed that the optimum salinity for P. sp. ANT319 was 9%. With salinity increasing, the total protein content and membrane permeability increased while SOD and POD enzymatic activities presented a trend of increasing firstly with the highest at 9% and then decreasing later, meanwhile CAT activity and MDA kept steady. Therefore, as the salinity increased, total proteins content increased as  result of cell membranes damaged gradually, meanwhile, the strain produced more proteins to remove harmful reactive oxygen species caused by salt stress and the activities of antioxidant enzymes were also enhanced. These results revealed the salinity tolerance mechanism of sea-ice bacteria preliminarily.
• Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase
• WANG Kai, LIU Hong-guo, JIANG Kun, YAN Pei-sheng*
• 2015, 5(3):  241-245.  DOI: 10.3969/j.issn.2095-2341.2015.03.16
• Abstract ( ) PDF (1182KB)( )
The bacterial strain FE10 producing lipase was screened from deep sea bacteria, which isolated from deep-sea sediment of South Atlantic. The molecular identification of 16S rRNA gene sequencing and phylogenetic analysis showed that FE10 belonged to the genus Marinobacter. The results of preliminary study on the lipase characterization showed that optimal temperature of the lipase activity was 40℃ under laboratory conditions. The lipase activity was high at pH 7, and almost disappeared at pH 9.