Table of Content

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• Current Biotechnology
  Volume 5 Issue 2 25 March 2015
• Progress on the Metagenomic Based on High-throughput Sequencing Technique Applicated in Animal Gastrointestinal Microorganisms
• WU Sen1, ZHANG Ying-ying1, ZAN Lin-sen1,2*
• 2015, 5(2):  77-84.  DOI: 10.3969/j.issn.2095-2341.2015.02.01
• Abstract ( ) PDF (1413KB)( )
Microorganisms are ubiquitous in nature, and have special function in degraded plant cell wall. In the animal gastrointestinal tract, the microorganisms is closely related with the growth and metabolism of the host, because of the degraded function contributed to transform plant to nutrition for the animal. Meanwhile, with the techniques and analysis methods improved, modern metagenomics, based on the high-throughout sequencing, was enable us to study microbia in complex environment more convenient. Using modern metagenomics techniques on the animal gastrointestinal microbial could conduce to a greater extent mining functional gene of microbial gene pool, which was not only played an important guiding role in the growth regulation, disease prevention and other basic science of animal, but also in the industrial production, food security and other fields. This review showed the research of metagenomic technology in animal gastrointestinal microbial classification, application etc., in order to provide scientific reference for animal production, microbial fermentation and other related field.
• The Background and Development Strategy of Microalgae Biodiesel
• WU Li-zhu1, DOU Shi-juan1, CONG Jun-guang2, YANG Guo-jun3, LIU Jian-feng1,4*
• 2015, 5(2):  85-88.  DOI: 10.3969/j.issn.2095-2341.2015.02.02
• Abstract ( ) PDF (1425KB)( )
Biodiesel is an important direction to develop and utilize renewable energy. Preparation biodiesel using microalgae has many advantages such as high output and growth speed, strong environment adaptability, not compete for land and water with food production. This article discussed something about biodiesel preparation using microalgae. And gave a brief introduction about raw materials for biodiesel production and development course, microalgae lipid composition and biosynthesis pathway, diesel preparing technology using microalgae, and its bottleneck and solution strategy. At last, the short and long-term development goals and prospects about microalgae biodiesel preparation technology was proposed.
• Progress on the Biosynthesis of Fungal Benzenediol Lactone Polyketides
• WANG Xiao-jing1, LU Wei1, XU Yu-quan1*, LIANG Xiao-dong2
• 2015, 5(2):  89-94.  DOI: 10.3969/j.issn.2095-2341.2015.02.03
• Abstract ( ) PDF (1748KB)( )
Fungal benzenediol lactones, biosynthesized by polyketide synthases, are important pharmacophores with wide-ranging bioactivities including anti-cancer and immune system modulatory effects. Its biosynthesis has become research focus in recent years. A comprehensive review was made about collaborative biosynthetic mechanism of two type I iterative polyketide synthases, and combinatorial biosynthesis of benzenediol lactones. As examples, the biosynthesis pathways of a few fungal benzenediol lactones were highlighted, which was expected provide reference for related research.
• Advances of Efficient Secretory Expression for Heterologous Proteins from Bacillus Species
• WANG Pei-li1,2, WANG Ping2, TIAN Jian2, WU Ning-feng2*, YAO Bin1
• 2015, 5(2):  95-102.  DOI: 10.3969/j.issn.2095-2341.2015.02.04
• Abstract ( ) PDF (2577KB)( )
Secreted proteins are easily to be purified and acquired a large amount with low cost, thus secretory expression of the target gene is an ideal expression model for the heterologous proteins. Bacillus expression system is one of the excellent heterologous protein expression systems, as most of the Bacillus species have the capability to secrete the target protein. So far, a large number of genes from different species have been cloned and achieved efficently secretory expression in Bacillus species. Many proteins even obtained very high yields. However, the protein secretory system of Bacillus still has many defects. The low yield for some proteins extremely limits its use as major “cell factories”in the biological field. Here we summarized the limiting factors for the secretion of the target protein and also provided some solutions for such problems.
• Progress on Germplasm Resources Genetic Diversity of Soybean
• HE Zhen1, YUN Xiao-dong1, WU Kai1, JI Hu-tai2, CHANG Jian-zhong1, QIAO Lin-yi1*, ZHENG Jun2*
• 2015, 5(2):  103-108.  DOI: 10.3969/j.issn.2095-2341.2015.02.05
• Abstract ( ) PDF (1042KB)( )
China is rich in soybean germplasm resources, great progress has been made on the genetic diversity research of soybean germplasm. In this paper, we summarized the progress on composition, conservation, research and utilization of soybean germplasm resources. The paper also discussed the importance and development direction of soybean genetic diversity, which was expected to provide reference for soybean breeding.
• Progress on Maize opaque-2 Related to Lysine and its Modifier Genes
• SHI Qing-ling, DONG Yong-bin*, ZHOU Qiang, MA Zhi-yan, QIAO Da-he, DENG Fei, LI Yu-ling*
• 2015, 5(2):  109-112.  DOI: 10.3969/j.issn.2095-2341.2015.02.06
• Abstract ( ) PDF (1091KB)( )
The maize o2 mutation increases the lysine content in the endosperm by decreasing the synthesis of zein storage proteins, which provide gene resourses for high lysine maize breeding. This paper reviewed the discovery, progress of o2 gene and its modifier genes, and summarized the progress of high lysine maize breeding using the interaction of the two genes. It was expected that the paper can provide reference for lysine maize breeding.
• Progress for Biomarkers and Detection Methods of Circulating Tumor Cell of Hepatocellular Carcinoma
• WANG Jiao-jiao1, GENG Yuan-yuan1, ZHANG Jia-yao2*, JIANG Wei1
• 2015, 5(2):  113-119.  DOI: 10.3969/j.issn.2095-2341.2015.02.07
• Abstract ( ) PDF (1099KB)( )
Hepatocellular carcinoma (HCC) is one of the high incidence and mortality rates of the malignant tumors in our country. Tumor resection or liver transplantation is the most effective means for treatment the disease. However, the high recurrence and transfer rates after operation influence seriously the prognosis of the patients. Circulating tumor cells (CTCs) in the peripheral blood is the important factor resulting in recurrence and metastasis of liver cancer after operation. In this paper, we reviewed the biomarkers of CTCs, e.g., glypican-3, transferrin receptor, alpha-fetoprotein, α-L-fucosidase, epithelial cell adhesion molecule, golgi protein, des-gamma-carboxy prothrombin, and the specificity and sensitivity for detection of CTCs using these biomarkers. The review was benefited for detecting the early cancer metastases, monitoring the recurrence, evaluating prognosis and choosing specific treatments for the patients with hepatocellular carcinoma.
• Construction and Transformation of High Efficient Gene Silencing Vector of Maize Starch Branching Enzyme Gene SBEⅡb and its Expression
• LI Nan1, ZHAO Ji-yuan1,2, YIN Yue-jia1, LIU Qing1, GUO Jia1, WANG Yun-peng1, LIU Xiang-guo1, HAO Dong-yun1, HE Hong-xia1*
• 2015, 5(2):  120-126.  DOI: 10.3969/j.issn.2095-2341.2015.02.08
• Abstract ( ) PDF (4992KB)( )
Starch branching enzyme (SBE) is a rate-limiting enzyme in the synthesis of starch. To declare the effect of SBEⅡb silencing on maize growth and amylase synthesis, SBEⅡb gene from Zea mays was cloned, and its expression vector pTFU-SBEⅡb containing hairpin structure was constructed. The construct was transformed into maize HiII immature embryos by Agrobacterium-mediated method. 194 regenerated plants were obtained through herbicide screening, 4 of them produced 35 seeds. Three positive plants were detected by PCR and BAR strip from T1 generation plants. Semi-quantitative RT-PCR results showed that the expression of SBEⅡb was reduced. And then, we deduced that the reducing level of SBEⅡb has positive effect on amylose synthesis.
• Functional Analysis of GmTMT2a Transgenic Maize Under Drought Stress
• ZHANG Liang1,2, ZHANG Lan2,WANG Xiao-ping1*
• 2015, 5(2):  127-131.  DOI: 10.3969/j.issn.2095-2341.2015.02.09
• Abstract ( ) PDF (1517KB)( )
Tocopherols are a group of powerful antioxidants, α-tocopherol is the most effective component. In this study, transgenic GmTMT2a plants (TP) with enriched-α-tocopherol and wild type plants (WT) were analyzed under drought stress. Results showed that H2O2 was accumulated in both TP plants and WT plants, however H2O2 content was less in TP plants than that in WT plants. We further analyzed the activity of antioxidant enzymes SOD, POD and CAT, and results showed that CAT actiity was obviously enhanced in TP plants compared with WT plants. Moreover P5CS and TPS transcripts were upregulated more in TP plants than WT. Our data showed that GmTMT2a increased drought tolerance of transgenic maize through promoting CAT, TPS and P5CS expression.
• Prokaryotic Expression and Optimization of Expression Conditions of BHMT Gene from Sus scrofa
• YU Ai-li1, ZHAO Jin-feng1, ZHANG Yan-meng2, ZHANG Zheng1, GUO Er-hu1
• 2015, 5(2):  132-136.  DOI: 10.3969/j.issn.2095-2341.2015.02.10
• Abstract ( ) PDF (6109KB)( )
Betaine-homocysteine methyl transferase (BHMT), which is a methyl-metabolizing enzyme , can catalyze methyl of betaine shifting to homocysteine, forming methionine. In this study, pET30a-SsBHMT was expressed in Escherichia coli. The strains, IPTG concentrations and induction time were optimized. The results showed that the expressed protein mainly existed in the form of inclusion bodies. After optimization, the target protein expression levels were increased both in supernatant and precipitate, but mainly in the form of inclusion bodies. In the strain of ER2566, the expression level of recombinant protein was high when induced for 10 h by 0.4 mmol/L of IPTG at 37℃. The results laid a foundation for further studies on the protein purification and antibodies preparation.
• Research on the Isolation, Identification and N-hexadecane Biodegrading Characteristics of Gordonia sp. LAM0048
• CHEN Xiao-rong1,2,3, RUAN Zhi-yong2,3*, WANG Yan-wei2, WANG Hui-min2, GUO Xiang2, KONG De-long2, SHAO Cheng-bin1*
• 2015, 5(2):  137-141.  DOI: 10.3969/j.issn.2095-2341.2015.02.11
• Abstract ( ) PDF (3306KB)( )
An n-hexadecane-degrading bacterium LAM0048 was isolated from hydrocarbon-polluted soil by using n-hexadecane as sole carbon source. Based on analyses of the morphological, physiological and biochemical character, cellular chemical composition, 16S rRNA sequences, strain LAM0048 was suggested to represent a member of the genus Gordonia of family Nocardiaceae, suborder Corynebacterineae. Comparative analysis of the fatty acids and polar lipid composition revealed that LAM0048T was a novel species within the genus Gordonia. Study on the degradation efficiency of strain LAM0048 with different n-hexadecane concentration indicated that strain LAM0048 was able to degrade 100% of n-hexadecane in the mineral medium containing 0.05% (V/V) n-hexadecane after 36 h incubation at 30℃, and was able to degrade 46.4% of n-hexadecane in the mineral medium containing 1.0% (V/V) n-hexadecane. The new isolate was an alkane-degrading bacterium with highly concentration of alkane tolerance ability，and may provide new insight into microbial degradation of alkane.
• Progress of Ebola Virus Vaccines and Analysis of Related Patents
• LI You-zhao, CAI Yu-pin, HAO Jia
• 2015, 5(2):  142-147.  DOI: 10.3969/j.issn.2095-2341.2015.02.12
• Abstract ( ) PDF (1516KB)( )
Through searching in the non-patent database，we summarized the latest research progress of the Ebola virus (EBOV), and depending on the patent analysis，we have understood the basic information about the key points which the inventors focus on. It was found that the amount of patent applications and outbreaks have some correlation；the patent applicant are more for the government-led or non-profit organization；the latest patent applications were concerned about the importance of the GP protein in the virus infection， antibodies and vaccines for GP proteins are being developed，among which Mapp biopharmaceutical companys products“Zmapp” are currently the best results as experimental biologic drugs.
• Field Efficacy Trials Report of 0.5% Veratridine Soluble Concentrate on Eggplants Red Spiders
• JIAO Xue-xia1, GU Pei-yun1*, WANG Yin2, YANG Jin-li1, ZHANG Chun-mei1, ZHENG Xiang3
• 2015, 5(2):  148-150.  DOI: 10.3969/j.issn.2095-2341.2015.02.13
• Abstract ( ) PDF (988KB)( )
Veratridine is a new type of botanical pesticides. A field experiment of 0.5% veratridine S C suppressing on eggplant red spider was conducted to verify the efficacy. The results showed that 0.5% veratridine solvent (×300) have control effect for red spider, control effect of 3 days after the first applying and 5 days after the second applying were low, however, 5 days after the last applying, the mean drug control efficacy reached 71.63%. Thus, this pesticide have poor quick result and good persistence effect when controlling the red spiders, and could be used before the high incidence of red spider in the spring, or used interchangeably with chemical pesticides to mitigate the effects of chemical pesticides on the environment and ecology.