Abstract: Shrimp processing waste was converted and deodorized for production of antioxidant polysaccharides by Cantharellus cibarius using response surface methodology. The optimal conditions for highest mycelium biomass and exopolysaccharides production were determined as follows: the solid to liquid ratio was 9.016%,  volume of liquid medium was 120.292 mL, and 1.208% concentration of glacial acetic acid. Under the optimal conditions, mycelium biomass and exopolysaccharides production reached 28.407±0.842 g/L and 7.009±0.517 g/L, respectively in validation test, which were consistent with the predicted values (RSD <5%). These values were improved by 60.564% and 114.737%, respectively compared to the initial culture conditions. The scores of deodorization were 3.056±0.115 and 3.694±0.076 at based and optimized validation bioconversion. The resulting exopolysaccharides had high antioxidant activities with total antioxidant capacity of 1.952±0.041 U/mg，superoxide anion scavenging capacity of 93.223±1.174 U/g and hydroxyl radical scavenging capacity of 60.291±0.638 U/mg. The study provided an alternative method for production of antioxidant polysaccharides from shrimp processing waste by Cantharellus cibarius.

Key words: shrimp processing wastes, bioconversion, deodorization, Cantharellus cibarius, antioxidant exo-polysaccharides

摘要： 利用响应面法研究了鸡油菌转化虾加工废弃物脱腥和抗氧化多糖的生产。生产菌丝体和胞外多糖的最优发酵条件为料液比9.016%, 装液量120.292 mL,冰醋酸含量1.208%，验证实验结果表明该条件下菌丝体产量为28.407±0842 g/L，多糖产量为7.009±0.517 g/L，分别较优化前提高了 60.564%和114.737%，而且与回归方程预测值接近(RSD<5%)。优化前去腥效果得分为 3.056±0.115， 优化后验证试验中得分为3.694±0.076。优化条件下获得的胞外多糖的总抗氧化能力为1.952±0.041 U/mg，抗超氧阴离子能力为93.223±1.174 U/g，抗羟自由基能力为60.291±0.638 U/mg。研究结果为利用鸡油菌转化虾加工废弃物生产抗氧化多糖提供了参考。

关键词: 虾加工废弃物, 生物转化, 脱腥, 鸡油菌, 抗氧化胞外多糖