Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase

doi:10.3969/j.issn.2095-2341.2015.03.16

Curr. Biotech. ›› 2015, Vol. 5 ›› Issue (3): 241-245.DOI: 10.3969/j.issn.2095-2341.2015.03.16

Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase

WANG Kai, LIU Hong-guo, JIANG Kun, YAN Pei-sheng

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Shandong Weihai 264209, China

Received:2015-04-01 Online:2015-05-25 Published:2015-04-24

产脂肪酶深海细菌的筛选鉴定及酶学性质研究

王凯,刘洪国,姜昆,闫培生*

哈尔滨工业大学（威海）海洋科学与技术学院, 山东威海 264209

通讯作者: 闫培生，教授，博士生导师，研究方向为海洋微生物资源与利用等。E-mail：yps6@163.com
作者简介:王凯，讲师，博士, 研究方向为环境微生物防治有害真菌的方法及机理、海洋功能细菌的多样性及应用。E-mail：ywkhit@hotmail.com。
基金资助:
中国大洋协会国际海域资源调查与开发“十二五”重大项目（DY125-15-R-01）；山东省优秀中青年科学家科研奖励基金（BS2014NY012）；哈尔滨工业大学学科建设引导基金（WH20140202）资助。

Abstract

Abstract: The bacterial strain FE10 producing lipase was screened from deep sea bacteria, which isolated from deep-sea sediment of South Atlantic. The molecular identification of 16S rRNA gene sequencing and phylogenetic analysis showed that FE10 belonged to the genus Marinobacter. The results of preliminary study on the lipase characterization showed that optimal temperature of the lipase activity was 40℃ under laboratory conditions. The lipase activity was high at pH 7, and almost disappeared at pH 9.

Key words: deep sea, microbes, lipase, 16S rRNA, enzymatic characteristics

摘要： 从分离自南大西洋深海沉积物的细菌中筛选出一株产脂肪酶菌株FE10，通过16S rRNA序列分析，对FE10进行分子鉴定和系统发育分析，初步确定其为海杆菌属（Marinobacter）。对菌株FE10所产脂肪酶进行酶学性质初步研究表明：在实验温度条件下所产脂肪酶40℃、pH 7时酶活最高，pH 9时酶活几乎消失。

关键词: 深海, 微生物, 脂肪酶, 16S rRNA, 酶学性质

WANG Kai, LIU Hong-guo, JIANG Kun, YAN Pei-sheng*. Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase[J]. Curr. Biotech., 2015, 5(3): 241-245.

王凯,刘洪国,姜昆,闫培生. 产脂肪酶深海细菌的筛选鉴定及酶学性质研究[J]. 生物技术进展, 2015, 5(3): 241-245.

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Study on Classification and Enzymatic Propertis of Deep-sea Bacteria Producing Lipase

产脂肪酶深海细菌的筛选鉴定及酶学性质研究

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