Table of Content

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• Current Biotechnology
  Volume 4 Issue 6 25 November 2014
• The Latest Research Progress of Myostatin in Mammals
• GAO Li, CHEN Yan, HU Ting-mao, LI Guang-peng*
• 2014, 4(6):  381-388.  DOI: 10.3969/j.issn.2095-2341.2014.06.01
• Abstract ( ) PDF (1694KB)( )
Myostatin, also known as growth/differentiation factor-8 (GDF-8), a member of transforming growth factor β (TGF-β) family, is mostly expressed in skeletal muscles and plays crucial roles in the negative regulation of muscle development. In this paper, we reviewed the latest research progress of MSTN in mammals, mainly discussed the relationship between MSTN signal pathway and other related signal pathways， and especially summarized the effect of myostatin on gene expression associated with muscle development under experimental conditions. This review also introduced the significance for knockout or knockdown of MSTN by genetic manipulation or using antibodies in increasing muscle growth in transgenic animals, clinic treatment of diseases such as muscular dystrophy, and delaying of aging.
• The Research Progress of Escherichia coli Expression Systems and Yeast Expression Systems
• ZHANG Yun-peng, WEN Tong, JIANG Wei*
• 2014, 4(6):  389-393.  DOI: 10.3969/j.issn.2095-2341.2014.06.02
• Abstract ( ) PDF (1672KB)( )
Since the 70s of 20th century, the rapid development of molecular biology and genomics have played increasingly important role in the field of biological and medical science. In fermentation industry，molecular biology techniques are widely used in construction of genetically engineered bacteria and strains improvement, in order to improve the yield of fermentation products and enrich fermentation product types. In the past decade, the use of prokaryotic and eukaryotic expression systems for exogenous gene amplification and expression to produce proteins vaccines, DNA vaccines and enzymic preparations, etc. is the emerging field of fermentation industry. In this paper, we reviewed the recent progress on E. coli expression systems and yeast expression systems from two aspects of vectors and host transformation.
• Strategy Discussion for CHO Cell Line Development
• TAO Wei-hong, QIN Min-min, ZHANG Zhe-ru
• 2014, 4(6):  394-399.  DOI: 10.3969/j.issn.2095-2341.2014.06.03
• Abstract ( ) PDF (1678KB)( )
Several strategies for industrial production of monoclonal antibody drug were introduced in this paper, including host cell selection, construction of expression vector, transfection method, screening techniques, cell culture technology as well as the standard method of final cell lines selection. On the basis of the progress of monoclonal antibody drug CHO cell line development and cell culture process, current strategy for the development of CHO cell lines in monoclonal antibody technology were also discussed.
• Affinity Maturation of Genetically Engineered Antibody by Gene Mutation
• DENG Long1, ZHOU Si2*, GUO Xin-Dong2
• 2014, 4(6):  400-404.  DOI: 10.3969/j.issn.2095-2341.2014.06.04
• Abstract ( ) PDF (1216KB)( )
Performance improvements of recombinant antibodies can be achieved by gene sequence analysis and modification. The methods for enhancing the affinity of recombinant antibodies through gene mutation were summarized, with special focus on the machanism analysis of small organic molecules-antibody interactions， antibody sequence databases and computer simulation based technologies，and a trending technology of molecular modeling and docking technology.
• Advances in Biological Activity of Cyclophilin A
• LIU Zi-yan, TIAN Hang-yu, XING Wen-xi, LIU Yue-tong, LI Ming*
• 2014, 4(6):  405-410.  DOI: 10.3969/j.issn.2095-2341.2014.06.05
• Abstract ( ) PDF (2815KB)( )
Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, School of Life Sciences, Yunnan University, Kunming 650091, China
• Application of ISSR Molecular Marker in Cotton Genetic Breeding
• SONG Zhi-hong, MENG Qing-zhong, ZHANG Tao, ZHANG Sheng-xi, WANG Gui-chun, LI Guo-rong*
• 2014, 4(6):  411-414.  DOI: 10.3969/j.issn.2095-2341.2014.06.06
• Abstract ( ) PDF (1198KB)( )
Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of Changjiang River (Wuhan), Ministry of Agriculture; Industrial Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China Inter-simple sequence repeat (ISSR) is a newly developed molecular marker method based on microsatellite sequences. This paper summarized the basic principles and characteristics of ISSR and reviewed its application in cotton genetic diversity detection, phylogenetic analysis, classification and identification of varieties, genetic mapping, gene tagging and marker-assisted selection breeding.
• Application of Klebsiella spp. in Agriculture and Environmental Management
• LI Meng-jiao1, PENG Sheng1, XU Shao-zhong2, YU Dai-hong3, ZHAO Ming-fu1*, WEN Guo-song2*
• 2014, 4(6):  415-420.  DOI: 10.3969/j.issn.2095-2341.2014.06.07
• Abstract ( ) PDF (1220KB)( )
Klebsiella spp. is an important member of Enterobacteriaceae family. It has theoretical research value and broader development prospects in agricultural field and environmental management. This paper reviewed on several key identification characteristics for major species of Klebsiellas spp., researches and existing problems of Klebsiella spp. in improving plant utilization of nitrogen，phosphorus, iron and other nutrients, biological control and environmental management， which would provide a reference for further research and application of this bacteria.
• Research Progress of Alexandrium tamarense, an Algae Species for Harmful Algal Blooms (HAB)
• ZHU Xing-biao1, ZHENG Yu-zhong2, XIE Li-ling1*, ZHANG Zhen-xia2, ZHA Guang-cai2, ZHANG Yong1, LIU Ya-qun1
• 2014, 4(6):  421-428.  DOI: 10.3969/j.issn.2095-2341.2014.06.08
• Abstract ( ) PDF (1280KB)( )
Alexandrium tamarense，a toxic dinoflagellate，is spreading all over the ocean in the world，including Bohai Sea，East China Sea，South China Sea and so on. A. tamarense is a major cause of toxic red tides，which can produce paralytic shellfish toxic (PST) that can be numerously accumulated in the bodies of fish and shellfish through food chain，and then bring serious harm to ocean environment or people health. Therefore，extensive and in-depth studies were conducted on the occurrence，development and toxin characteristics of A. tamarense. This paper summarized the growth environment，molecular identification，algal interspecific competition relationship and governance of A. tamarense，with the aim to provide references for further understanding and control of this alga.
• Genetic Diversity and Phylogeny Analysis of Duolang Sheep Based on Mitochondrial DNA-loop Variation
• WANG Shi-feng, LI Jing, WANG Yu-tao
• 2014, 4(6):  429-434.  DOI: 10.3969/j.issn.2095-2341.2014.06.09
• Abstract ( ) PDF (2399KB)( )
We used the Duolang sheep as object in this study，researched the genetic diversity of mitochondrial D-loop region of sheep，layed the foundation for the study of the origin and evolution of Duolang sheep. The results showed that the length of mtDNA D-loop is 945~1 039 bp.　The percentage of A, T, G, C was 29.4%, 27.7%, 17.7% and 25.1%, respectively. A+T was 571%, 　G+C was 42.8%. The study acquired 26 haplotypes, 56 polymorphic sites. Single polymorphism sites and concision information sites were 42 and 14 respectively. The average number of nucleotide differences k is 5.289, nucleotide diversity P is 0.024 15, which indicated that genetic diversity of Duolang sheep was low and key protection measures should be taken. Furthermore, it was also found that Duolang sheep experienced population expansion, there might be D or E lineage of the matrilineal origin besides A, B and C lineage.
• Isolation of Aerobic Denitrifying Bacteria from Sediment in Erhai Lake and Exploration of Its Denitrification Capacity
• KUI Yi-ping1, ZHANG Wen2, LIN Li-jia2, NI Zhao-lin2, SHEN Yuan-ying2*
• 2014, 4(6):  435-438.  DOI: 10.3969/j.issn.2095-2341.2014.06.10
• Abstract ( ) PDF (1396KB)( )
Strains with strong aerobic denitrifying ability was isolated from sediments in Erhai lake. 101 denitrifying bacteria was screened out from sediments in ten sampling points of Erhai lake through enrichment culture，and among which，1 strain with strongest denitrification ability was selected and named EH314. Test on this bacteria including contacting enzymes (catalase)，producing hydrogen sulfide and starch hydrolysis showed positive results，fermentation of glucose oxidation results showed it an oxidizing bacteria，results of producing lipase (Tween 80) test were negative； the strain EH314 was identified as Alcaligenes bacteria； bacterial denitrification capacity was measured and found to effectively degrade nitrate in water by the strain EH314, and the denitrification was under aerobic conditions.
• Fermentation and Bacteriostatic Experiment of Sodium Gluconate
• FENG Wen-hong, ZHOU Sheng-min, GAO Zhao, FANG Jian, DUAN Ying-ying, CAO Da-peng, ZHAO Wei*
• 2014, 4(6):  439-442.  DOI: 10.3969/j.issn.2095-2341.2014.06.11
• Abstract ( ) PDF (1189KB)( )
Sodium gluconate was produced through fermentation by Aspergillus niger. Fermentation parameter was detected and monitored to study the variation of the parameters of fermentation and physiological activities of Aspergillus niger. The fermentation process was optimized through the orthogonal test. The optimum conditions were as follows: 16.5 h seed; 15% inoculation quantity, pH 5.8. Antibacterial effect of sodium gluconate was verified, and the results showed that the amount of sodium gluconate as feed additive could be 0.5% to 0.7%, which provide a theoretical basis for the application of sodium gluconate in the feed industry.
• Exploration of Surface Sterilization Methods for Isolation of Endophytic Fungi from Taxus chinensis var. mairei
• LI Jun-qin1,2, YIN Xiao-wen1, SONG Pei-yong1*
• 2014, 4(6):  443-446.  DOI: 10.3969/j.issn.2095-2341.2014.06.12
• Abstract ( ) PDF (1183KB)( )
Surface sterilization is the key step in isolation of endophytes from plants. We explored a new suface sterilization method to isolate endophytic fungi from Taxus chinensis var. mairei with 75% ethanol and 2% sodium hypochlorite (NaClO) instead of mercuric chloride. After pretreated by immersing in 75% ethanol for 3 min, the yew leaf，stem and bark were all immersed in 2% sodium hypochlorite solution for 2 min, and then immersed in 75% ethanol for 3 min，5 min and 7 min，respectively. The method has a good surface sterilization effect.