Abstract: Performance improvements of recombinant antibodies can be achieved by gene sequence analysis and modification. The methods for enhancing the affinity of recombinant antibodies through gene mutation were summarized, with special focus on the machanism analysis of small organic molecules-antibody interactions， antibody sequence databases and computer simulation based technologies，and a trending technology of molecular modeling and docking technology.

Key words: genetically engineered antibody, molecular modeling, gene mutation, affinity maturation

摘要： 基因工程重组抗体可通过对基因序列的分析、修饰与修改，从而改进抗体特性。对基于基因突变的基因工程抗体亲和力成熟的方法进行总结，主要包括小分子有机物-抗体相互作用机制分析、抗体序列数据库及计算机模拟技术，以及新兴的分子模拟与对接技术。

关键词: 基因工程抗体, 分子模拟, 基因突变, 亲和力成熟