Abstract: Several strategies for industrial production of monoclonal antibody drug were introduced in this paper, including host cell selection, construction of expression vector, transfection method, screening techniques, cell culture technology as well as the standard method of final cell lines selection. On the basis of the progress of monoclonal antibody drug CHO cell line development and cell culture process, current strategy for the development of CHO cell lines in monoclonal antibody technology were also discussed.

Key words: monoclonal antibody, CHO cell, cell line development, cell culture process

摘要： 主要介绍了单克隆抗体药物工业生产中宿主细胞选择、表达载体构建、转染方法、筛选技术、细胞培养工艺技术方法以及最后选定细胞株的标准等，结合单抗药物CHO细胞株开发和培养工艺的经验，对当前我国单抗CHO细胞株开发技术策略进行了探讨。

关键词: 单克隆抗体, CHO细胞, 细胞株开发, 培养工艺