Application of ISSR Molecular Marker in Cotton Genetic Breeding

doi:10.3969/j.issn.2095-2341.2014.06.06

Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (6): 411-414.DOI: 10.3969/j.issn.2095-2341.2014.06.06

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Application of ISSR Molecular Marker in Cotton Genetic Breeding

SONG Zhi-hong, MENG Qing-zhong, ZHANG Tao, ZHANG Sheng-xi, WANG Gui-chun, LI Guo-rong

Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of Changjiang River (Wuhan), Ministry of Agriculture; Industrial Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China

Received:2014-07-15 Online:2014-11-25 Published:2014-09-03

ISSR分子标记在棉花遗传育种上的应用

宋志红,孟庆忠,张涛,张胜昔,王贵春,李国荣*

湖北省农业科学院经济作物研究所, 农业部长江中游棉花生物学与遗传重点实验室(武汉), 武汉 430064

通讯作者: 李国荣，研究员，主要从事棉花育种研究。E-mail：lgr1lgr@qq.com
作者简介:宋志红，副研究员，硕士，主要从事棉花育种研究工作。E-mail:1225493533@qq.com。
基金资助:
转基因生物新品种培育科技重大专项(2013ZX08005-001-006)；国家863计划项目（2011AA10A102）；湖北省农业综合开发项目；湖北省农业科技创新中心资助项目(2007-620-001-03)资助。

Abstract

Abstract: Key Laboratory of Cotton Biology and Breeding in the Middle Reaches of Changjiang River (Wuhan), Ministry of Agriculture; Industrial Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China Inter-simple sequence repeat (ISSR) is a newly developed molecular marker method based on microsatellite sequences. This paper summarized the basic principles and characteristics of ISSR and reviewed its application in cotton genetic diversity detection, phylogenetic analysis, classification and identification of varieties, genetic mapping, gene tagging and marker-assisted selection breeding.

Key words: ISSR, molecular marker, cotton, genetic breeding

摘要： ISSR是一种近些年发展起来的基于微卫星序列的新型分子标记。阐述了ISSR的基本原理、特点及其在棉花遗传多样性与亲缘关系分析、种质鉴定、遗传图谱构建、基因定位及标记辅助选择育种等方面的应用和进展。

关键词: ISSR, 分子标记, 棉花, 遗传育种

SONG Zhi-hong, MENG Qing-zhong, ZHANG Tao, ZHANG Sheng-xi, WANG Gui-chun, LI Guo-rong*. Application of ISSR Molecular Marker in Cotton Genetic Breeding[J]. Curr. Biotech., 2014, 4(6): 411-414.

宋志红,孟庆忠,张涛,张胜昔,王贵春,李国荣. ISSR分子标记在棉花遗传育种上的应用[J]. 生物技术进展, 2014, 4(6): 411-414.

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Application of ISSR Molecular Marker in Cotton Genetic Breeding

ISSR分子标记在棉花遗传育种上的应用

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