Abstract: Surface sterilization is the key step in isolation of endophytes from plants. We explored a new suface sterilization method to isolate endophytic fungi from Taxus chinensis var. mairei with 75% ethanol and 2% sodium hypochlorite (NaClO) instead of mercuric chloride. After pretreated by immersing in 75% ethanol for 3 min, the yew leaf，stem and bark were all immersed in 2% sodium hypochlorite solution for 2 min, and then immersed in 75% ethanol for 3 min，5 min and 7 min，respectively. The method has a good surface sterilization effect.

Key words: Taxus chinensis var. mairei, surface sterilization method, endophytic fungi, paclitaxel

摘要： 表面消毒是植物内生菌分离的关键环节，在南方红豆杉(Taxus chinensis var. mairei)内生真菌分离的过程中，使用75％乙醇、2％次氯酸钠（NaClO）代替升汞对外植体进行了表面消毒摸索。结果显示：红豆杉叶、茎、皮经预处理后，先用75％乙醇浸泡3 min，再用2％次氯酸钠浸泡2 min，然后红豆杉叶、茎、皮分别用75％乙醇浸泡3 min、5 min、7 min，可取得满意的表面消毒效果。

关键词: 南方红豆杉, 表面消毒方法, 内生真菌, 紫杉醇