-
Application of Biotechnology in the Culturing, Processing and
Preservation of Aquatic Products
- JIN Huo-xi, ZHANG Zhi-guo*, GAO Hai-yan
-
2013, 3(6):
389-392.
DOI: 10.3969/j.issn.2095-2341.2013.06.02
-
Abstract (
)
PDF
(1754KB)(
)
Biotechnology, especially enzyme engineering technology, has
been widely used in the development of aquatic products. This paper
reviews on applications of biotechnology in aquaculture, processing and
preservation. In aquaculture, biotechnology is mainly used for feed
production and water quality control; in aquatic products processing,
biotechnology is applied for tissue degradation in aquatic products, odor
removal, protein and functional ingredients production and preparation of
flavor seasonings; in seafood preservation, a variety of enzymes
preservation technology have been developed. The application of
biotechnology will enable aquaculture, processing and preservation
industries to develop more fast.
-
Progress on Antioxidant Capacity of Postharvest Peach Fruit Regulated by
Respiratory Metabolism
- LIANG Min-hua1,2, YANG Zhen-feng2*, CHEN Wei2, SU Xin-guo3
-
2013, 3(6):
416-420.
DOI: 10.3969/j.issn.2095-2341.2013.06.08
-
Abstract (
)
PDF
(1120KB)(
)
Respiratory metabolism is one of the most important
physiological activities of posthavest climacteric fruit, and is also
closely related to the biosynthesis of different bioactive compounds.
This paper concluded the effect of respiratory metabolism on the
biosynthesis and metabolism of antioxidant composition (such as phenolic
acid, flavonol, flavan-3-ol, anthocyanin and carotenoids) and key
enzymes expressions in postharvest peach fruit of different varieties
(Red-flesh, Yellow-flesh, and White-flesh). Based on investigations
from the respiration pathway, the paper also discussed the specific
mechanism of respiratory metabolism involved in the biosynthesis of such
antioxidant compounds, expounded the molecular basis of the biosynthetic
pathway and regulation of key enzymes of flavonoids and carotenoids, and
clearifed the role and regulation of storage environmental conditions on
the antioxdiant properties and antioxidant compounds of harvested fruits
during storage. These would provide theoretical basis for regulation of
antioxidant compounds metabolism and finally improve the nutritional
quality in postharvest fruits.
-
Purification of Ganoderma lucidum Polysaccharides by Coupling
Ultrafiltration with Radial Flow Chromatography
- SHAO Ping1, LIU Qing1, CHEN Chun-bin2, QIN Min-pu1, SUN Pei-long1
-
2013, 3(6):
427-432.
DOI: 10.3969/j.issn.2095-2341.2013.06.10
-
Abstract (
)
PDF
(1625KB)(
)
The removal of protein and the retention rate of polysaccharide
in Ganoderma lucidum polysaccharides hydrolyzate were studied using
ultrafiltration and coupling method. Using the ultrafiltration membrane
with a cut-off molecular mass of 100 kDa, the effects of several
parameters such as the flow rate, ultrafiltration time and concentration
multiple on the ultrafiltration were evalutated. The optical conditions
of removing protein by ultrafiltrating were determined. It is founded
that the flow rate, time and concentration multiple were 100 mL/min, 60
min,15~17 times, respectively, at the room temperature and the pressure
less than 30 psi. The rate of protein removal reached 82.21% and the
retention rate of polysaccharide was 63.32%. Coupled the ultrafiltration
with radial flow chromatography, the retention rate of polysaccharide and
the removal rate of protein were as high as 99.24% and 89.52%. As
compared with the single chromatography and the Sevag method, the
efficiency increased. These findings thus clearly suggested purification
of polysaccharides from Ganoderma lucidum by coupling ultrafiltration
with radial flow chromatography was feasible with high retention rate.
