Table of Content

• 
• Current Biotechnology
  Volume 4 Issue 1 25 January 2014
• Research Progress of Fiber\|specific Gene and Promoter from Cotton
• LV Shao-pu, WANG Xu-jing, TANG Qiao-ling, WANG Zhi-xing*
• 2014, 4(1):  1-6.  DOI: 10.3969/j.issn.20952341.2014.01.01
• Abstract ( ) PDF (1286KB)( )
Cotton is an important cash crop. Since it is difficult to improve the yield and quality of cotton simultaneously using conventional breeding methods, combination of genetic engineering method and conventional breeding method is a practical approach to improve the quality of cotton fiber quality currently. This paper summarized the process of fiber development of cotton, and reviewed the specific genes and promoters involved in fiber development, so as to provide references for improving the quality of cotton fiber by means of genetic engineering method.
• Progress on Detection Technologies and Safety Evaluation of Phthalic Acid and Adipate Acid Esters in Organism
• CAI Yan1, HE Xiao-yu2, YANG Zhen-feng1*, SU Xin-guo3
• 2014, 4(1):  7-11.  DOI: 10.3969/j.issn.2095-2341.2014.01.02.
• Abstract ( ) PDF (1257KB)( )
The polymer plastic products, phthalic acid esters (PAEs) and adipates acid esters (AEs), have been increasingly used in household and industrial plastic additives. The presence of PAEs and AEs can be detected in the global ecological environment, including air, soil, water, food and even organisms. This paper summarizes and compares the pretreatment and detection methods of PAEs and AEs in recent years, and reviews the safety evaluation of PAEs and AEs in the organism.
• Development of Biological Technology on Fruit and Vegetables Preservation
• 2014, 4(1):  12-16.  DOI: 10.3969/j.issn.2095-2341.2014.01.03.
• Abstract ( ) PDF (1266KB)( )
With great concern on food security, fresh\|keeping technology developed rapidly, and it became a research trend to seek safe and efficient biopreservation technologies. This paper introduced the principle of biological preservation; reviewed the application of biological technology on fruites and vegetables preservation, including the microognisms and their metabolites，the natural extract and genetic engineering technology; then discussed the deficiencies and development directions of biological preservation technology.
• The Application of Proteases on the Processing of Aquatic Products
• YU Li-na, SHAO Xing-feng*
• 2014, 4(1):  17-21.  DOI: 10.3969/j.issn.2095-2341.2014.01.04.
• Abstract ( ) PDF (1245KB)( )
Low-value aquatic products and by-product of fish can not be fully utilized by traditional processing technology, resulting in a waste of resources. As a safe, efficient and speedy processing technology， proteases digestion technology is widely used to improve product efficiency and product quality on the processing of aquatic products. This paper reviews the application status of proteases on the processing of low-value aquatic products and by-product of fish, especially in hydrolyzing fish protein, extracting biologically active substances and preparing aquatic flavouring. It also proposed some precautions on the processing of protease digestion and predicted the development of proteases aim to as a reference for aquatic products processing.
• Bioinformatics Analysis of PRMT Protein Family in Maize
• QIAN Ye-xiong， XU Shi-jie， ZHANG Ya-nan， ZHANG Meng-fei
• 2014, 4(1):  22-29.  DOI: 10.3969/j.issn.2095-2341.2014.01.05.
• Abstract ( ) PDF (5111KB)( )
Protein arginine methyltransferases, PRMTs, are one of important epigenetic regulating factors，which are involved in modifying histone arginine methylation, changing chromatin structure and regulating gene expression in eukaryotic genome. In this study, a complete set of 8 PRMT proteins were identified in maize genome. By contrasting with the PRMT protein sequences in Arabidopsis and rice, all 8 ZmPRMT proteins were categorized into three classes based on phylogeny in maize. By using bioinformatics methods and softwares, physical and chemical of amino acid sequence of all maize PRMT proteins were analyzed, and their signal peptide, transmembrane domain, hydrophobic and hydrophilic structure, protein secondary and tertiary structure were also determined in this study. These results revealed a comprehensive overview of the maize PRMT protein family and provided a great significance on subsequent functional research of PRMT proteins in maize.
• Effects of Nitric Oxide Treatment on Polygalacturonase Activity and Expression of MaPGs Genes in Harvested Banana Fruit During Storage
• WU Bin1,2, GUO Qin3, WANG Gang-xia2, CHEN Wei-xin1, LI Xue-ping1*
• 2014, 4(1):  30-35.  DOI: 10.3969/j.issn.2095-2341.2014.01.06
• Abstract ( ) PDF (1453KB)( )
In order to explore the molecular regulation mechanism of polygalacturonase (PG) during banana softening, fruit harvested at the mature green stage were fumigated with 40 μL/L of NO for 3 h, and then stored at 20℃ with 85% relative humility. Ethylene production, firmness, PG activity and MaPGs genes were measured during banana ripening. The results showed that NO treatment resulted in significantly lower rates of ethylene production than those in the control fruit. The NO treated fruit underwent a lesser loss of firmness during storage. The activity of PG and expression of MaPG2、MaPG3 and MaPG4 genes were effectively reduced in NO treated fruit, indicating that the softening of banana fruit was delayed by NO treatment.
• Effects of Multi\|function Streptomyces Biological Organic Fertilizer on Growth of Old Pear Tree and Culturable Rhizosphere Bacteria and Endophytic Bacteria of Root
• HU Dong, QU Ya-li, ZHANG Cui-mian, JIA Nan, LI Hong-tao, WANG Zhan-wu*
• 2014, 4(1):  36-39.  DOI: 10.3969/j.issn.2095-2341.2014.01.07
• Abstract ( ) PDF (1229KB)( )
Over the past three consective years from 2010 to 2012, we have been taking application test which used the multi\|function Streptomyces TOR3209 as the primary biological organic fertilizer on pear trees for more than 25 years. The results showed that TOR3209 could promote the growth of old pear trees, which was reflected in the significant increase of regeneration roots and capillary roots; leaves increased in thickness and leaf biomass, the color turned from yellow to green, and the content of chlorophyll increased by 14.6%; the quality of fruits improved largely, that the content of soluble solids increased by 6.3%, and the firmness of fruits increased by 10.8%; the diversity of culturable bacteria in roots and rhizosphere soil also increased significantly. From above, we can drew the conclusion that multi\|functional biological organic fertilizers contributed to repairing and improving the growth of old pear trees through rhizosphere ecosystem, it had an expansive application prospect in the field of orchard improvement.
• Screening of Pancrelipase Inhibitor\|producing Strains
• SUN Fei, ZHENG Rong, YANG Huang-jian, ZHANG Zhu-lan*
• 2014, 4(1):  40-43.  DOI: 10.3969/j.issn.2095-2341.2014.01.08
• Abstract ( ) PDF (2062KB)( )
A pancreatic lipase inhibitor screening model was constructed, which combined with determination of inhibitory activities of amylase, protease and lipase. Three strains FIM-1013, FIL-19 and FIMe-3 were obtained through target screening of microbial broths from soil organisms by this model. The lipase inhibitory activites of FIM-1013,FIL-19 and FIMe-3 were 61%,53% and 42%, the amylase inhibitory activites were 0,7% and 3%, and the inhibitory protease activity were 6%,4% and 0. The results indicated that the three strains could produce very potent selective inhibitor of pancreatic lipase which deserved further research.
• Sreening of Crude Oil Degrading Bacteria and its Degradation Capability
• WU Pei-chun1, WANG Hai-tao1, LIU Ya-nan1, XIN Yan-juan1, CAO Xu-peng1, XUE Song1*
• 2014, 4(1):  44-48.  DOI: 10.3969/j.issn.2095-2341.2014.01.09
• Abstract ( ) PDF (2270KB)( )
A strain of crude oil degrading bacteria OA58 was isolated from a crude oil contaminated marine sponge from Dalian Bay, China. According to the 16S rRNA genes sequences analysis, physiological and biochemical characterizations, the strain OA58 was identified as Streptomyces exfoliatus. When the initial concentration of crude oil was 1 g/L, the strain OA58 could consume 83% of the crude oil in 14 days, indicating a good potential of degradation capability of crude oil.
• Temperature and Humidity Control System of Digital Treatment Earthworm Bioreactor based on SVM and MPC
• CAI Yong-ming, GU Ling-yan
• 2014, 4(1):  49-53.  DOI: 10.3969/j.issn.2095-2341.2014.01.10
• Abstract ( ) PDF (1345KB)( )
In order to auto control temperature and humidity of the earthworm biological treatment of municipal sludge treatment reactor. By using of SVM (support vector machine) and MPC (model predictive control) technology, an automatic control model was constructed. The temperature and relative humidity of the earthworm biological reactor could be controlled accurately according to the set point. The method can be used to control the temperature and relative humidity of the reactor.
• GC-MS Analysis of Biomass Tar Distillate Composition under Different Temperature
• YANG Yu-qiong, LU Shi-yuan
• 2014, 4(1):  54-58.  DOI: 10.3969/j.issn.2095-2341.2013.0 .
• Abstract ( ) PDF (1290KB)( )
The production of biomass tar caused clogging, pollution and corrosion in the gas pipeline and gas cooker, which restricted the sustainable development of biomass energy industry. The composition of biomass tar distillate produced during biomass gasification process in straw gasifier designed by Agricultural Machinery Institute was analyzed by GC/MS method for four temerature ranges of 110~160℃, 160~160℃, 200~210℃ and 210~220℃. The results showed that there were 16 kinds of qualitative compounds, which were methylbenzene and 14 kinds of phenolic derivatives, and one kind of fatty acid ester. This study provided reference for tar processing method.
• Determination of Phosphatidylcholine in Zophobsmorio L. by Liquid Chromatography
• FAN Zhi-ying, SHI Dong-dong*, CHANG Bi-ying, ZHANG Ping
• 2014, 4(1):  59-62.  DOI: 10.3969/j.issn.2095-2341.2014.01.12
• Abstract ( ) PDF (1356KB)( )
A liquid chromatographic method for determination of phosphatidylcholine in Zophobsmorio L. has been developed. The samples were analyzed by liquid chromatography. Quantitative method was external standard method. The linearity was checked by standard solutions at concentration range from 250～2 000 μg/mL, the correlation coefficient was 0.999 6. The recovery values ranged from 97% to 109% with relative standard deviations(RSDs) no larger than 2.4%. For different growth stage of Zophobsmorio L., the results of the content of phosphatidylcholine in vivo showed that the content of phosphatidylcholine was in line with the binomial curve along with the change of the weight, the correlation coefficient R2 was 0.865 (P<0.01).
• Evaluation of Application Time for Pythium oligandrum Against Cucumber Downy Mildew in Greenhouse
• SUN Hai, WANG Xiao-qing, LI Yun-long, HU Bin, ZHENG Jian-qiu
• 2014, 4(1):  63-65.  DOI: 10.3969/j.issn.2095-2341.2014.01.13
• Abstract ( ) PDF (1213KB)( )
Pythium oligandrum is a newly biopesticide which shows great effects against many kinds of plant diseases. In greenhouse experiment, we found that Pythium oligandrum had different suppressing effects on cucumber downy mildew following different application time. The result showed that the control effect of Pythium oligandrum on cucumber downy mildew reached 68.9% when applicating the biopesticide Pythium oligandrum before cucumber downy mildew occurred. In comparison, the control effect only reached 23.5%, and the cucumber downy mildew had occurred seriously. It is better to apply Pythium oligandrum before or in the early stage of downy mildew pathogenesis occurring for better disease control.