Current Biotechnology

Progress in Using Plant Bioreactor to Produce Pharamatical Protein
LIU Pei-lei1, LI Ning1, LIAN Qing1, WANG Xu-jing2, WANG Zhi-xing2*
2013, 3(5): 309-316. DOI: 10.3969/j.issn.2095-2341.2013.05.01
Abstract ( ) PDF (1241KB)( )

Plant bioreactor was much concerned because of the characteristics of low cost, safety, the same immunogenicity and biological activity of the expressed products compare with animal bioreactor, etc. It is a new trend in plant genetic engineering by using plant bioreactor to produce pharmaceutical proteins. A variety of products are studies on clinical stage. In this paper, we reviewed the recent studies on pharmaceutical protein producing by plant bioreactor, analyzed the current problems existing in plant bioreactor, and prospected the application of this technology in future.

Progress in Rabies Virus Glycoprotein and Neutralizing Antibody
CHANG Liang, LIU Xiao-zhi, GAO Jian
2013, 3(5): 317-323. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (1565KB)( )

Rabies is a fatal zoonosis caused by rabies virus (RV). Rabies virus glycoprotein (RVG), the unique viral protein exposed on the surface of viral envelope, can induce the production of rabies neutralizing antibodies. This review focuses on recent advances in rabies virus, the structure and function of glycoprotein, progress of neutralizing antibodies and their action mechanism, which can provide theoretical foundations and development strategies for further study of neutralizing antibodies.

Advance and Application of FOX Gene Hunting System in Plant
LIU Ya-yun, WU Jin-xia, LU Tie-gang*
2013, 3(5): 324-327. DOI: 10.3969/j.issn.2095-2341.2013.05.03
Abstract ( ) PDF (1598KB)( )

Large numbers of mutant plant populations have been generated by ‘gain-of-function’ techniques. Great progress have been made in Arabidopsis and rice through the use of these tools. Using a single or a limited number of full length cDNAs, FOX system makes gene ectopic expression in plant, accessing functional mutants, especially the gain-of-function mutant. The FOX gene hunting system have been maturely used not only in model plant species,such as Arabidopsis and rice, but also in other plant species.

Advanced Study on the Male Sterility of Cotton
SHI Ya-li, ZHANG Rui, MENG Zhi-gang, ZHOU Tao, SUN Guo-qing, MENG Zhao-hong, GUO San-dui*
2013, 3(5): 328-335. DOI: 10.3969/j.issn.2095-2341.2013.05.04
Abstract ( ) PDF (1209KB)( )

There is fully apparent heterosis in cotton, showing on the production, quality and physiological characteristics of cotton. The male sterility on cotton has played an important role in heterosis usage in cotton. Utilization of the male sterile system have made a breakthrough in conquering breeding bottleneck by artificial emasculation and unfolded a bright outlook for promoting commercial production of hybrid seed. This paper reviewed the major advances of biological research on the male sterility in cotton in the past decade; focused on the types, cytological features, mechanisms and breeding method of male sterile cotton; analyzed the existed problems; and maked prospects of male sterility applications.

Progress on Correlation Between Starch Synthase and Starch Synthesis in the Grain
WANG Zi-bu, HUANG Yan-fen, WU Kun, REN Cui-juan, JIANG Jin-zhong*
2013, 3(5): 336-341. DOI: 10.3969/j.issn.2095-2341.2013.05.05
Abstract ( ) PDF (1203KB)( )

Starch biosynthesis is a complex biochemical process that involves in a series of enzymes reaction. This paper reviews the function of starch synthase (SSS), starch branching enzymes (SBE), starch debranching enzymes (DBE) and their isoenzymes in the process of starch synthesis, and discusses the relationship between starch synthase and starch synthesis pathway in the grain, then prospects the research focus on the studies of complex biochemical reactions in starch synthesis pathway.

Prospect of Flowers Soilless Culture in China
LIU Guo-rong
2013, 3(5): 342-345. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (1134KB)( )

Flowers soilless culture technology, which is a relatively new technology, has entered the practical application in production. This paper analyzed the advantages and disadvantages of flowers soilless cultivation, reviwed on soilless cultivation types and supporting technologies, introduced the applications in brief, then pointed out the existed problems, and prospected the future development of flowers soilless culture in China. Finally, some reasonalble suggestions were proposed to contribute to the development of modern flower industry.

Research Progress on Physiological Functions of Anthocyanins and their Application Prospects
ZHONG Lan-lan, TU Di, YANG YA, LIU Jin-hui*
2013, 3(5): 346-352. DOI: 10.3969/j.issn.2095-2341.2013.05.07
Abstract ( ) PDF (1241KB)( )

Anthocyanins are a kind of watersoluble plant pigments with some special unique physiological functions, so as to have big attentions. This paper reviews the biosynthetic pathway of anthocyanin and related enzymes, focuses on the physiological functions and mechanisms of anthocyanins, such as antioxidant, liver function protection, cardiovascular disease prevention, anticancer, antiinflammatory, antiinfection and eye protection, and so on. Finally, it makes an prospects on the application of anthocyanins in medicine, food and cosmetics industries in the future.

Study on Superovulation of Different Varieties of Goats
GAO Wen-chao1, WANG Xue-zhu2, ZHANG Sheng-gang2, QU Lei3, GAO Zhi-min1, LEI An-min1*, YIN Hai-ke2, WANG Zhong-lin2, REN Bao-hua2
2013, 3(5): 353-356. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (1142KB)( )

To compare the Boer goat and Shaanbei white cashmere goat superovulation effect in the commercial application, 29 Boer goats and 8 Shaanbei white cashmere goats were superovultated with different FSH made in different countries. Superovulatory treatment was performed using Ca-FSH in Boer goats and Shaanbei white cashmere goats. The total number of recovered embryos per does was 12.80±7.98 for Boer goats, which was higher than that of Shaanbei white cashmere goats (9.43±7.16), but without significant difference (P>0.05). The results also showed that the estrous rate of the treated donor after operated were badly influenced by the collection method from oviduct. So the collection method of the uterine horn ought to be preferentially used in goat.

Identification of Powdery Mildew Resistant Genes based on SCAR Markers in Common Bean (Phaseolus vulgaris L.)
WU Xing-bo1,2, HAO Jun-jie1*, ZHANG Xiao-yan1, WAN Shu-wei1, LI Hong-wei1, SHAO Yang1, SUN Ji-lu1
2013, 3(5): 357-362. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (1453KB)( )

Eight SCAR primer combinations（SAU5, SS18, SF6Em3, SF12R9, SF13R10, SF18R7, SF18R15 and SMe1Em5）of common bean powdery mildew resistant genes were used to check the genome DNA of 78 common bean accessions. The SCAR primer pairs of SF12R9, SF13R10, SF18R7, SF18R15 and SMe1Em5 did not amplified target bands at all; SAU5 marker appeared in 52 accessions, SF6Em3 marker in 66 accessions, and SS18 marker in 76 accessions. 2~3 SCAR markers appeared in 76 accessions respectively. The types of powdery mildew resistant genes in each of the 78 accessions have been identified, and the accessions with pyramiding powdery mildew resistance genes were selected.

Genetic Environment of bla_KPC-2 in the Intestinal Colonized Raoultella planticolas
GE Chao-rong
2013, 3(5): 363-367. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (2626KB)( )

The increasing emergence and spread of Klebsiella pneumoniae carbapenemases (KPCs) leaves fewer available therapeutic options due to their broad-spectrum hydrolytic activity. Two KPC-2-producing Raoultella planticola isolates were first identified and analyzed using PCR, Southern hybridization and sequencing in China. Two isolates had an identical antimicrobial susceptibility profile, bla_KPC-2 gene as well as its flanking sequences. They were resistant to 16 antimicrobial agents while sensitive to polymyxin B, colistin and minocycline. The results demonstrated that the bla_KPC-2 gene was located on a 7 498 bp plasmid segment that encoded five open reading frames with the order of Tn3-transposase, Tn3-resolvase, ISKpn8, bla_KPC-2 gene and ISKpn6-like.

Effects of Temperature on Growth and Physiological Biochemical Compositions of Cylindrotheca fusiformis
CHEN Shu-xiu
2013, 3(5): 368-370. DOI: 10.3969/j.issn.2095-2341.2013.05.11
Abstract ( ) PDF (1146KB)( )

Effects of temperature on growth and physiological biochemical compositions of cylindrotheca fusiformis were studied. The results indicated that the optimal growth temperature range for cylindrotheca fusiformis was 15~30℃. The optimal growth temperature under the experimental conditions was 30℃.The contents of the chlorophyll and the extracellular polysaccharide gained maximum level at 25℃, but the intracellular protein and the intracellular polysaccharide gained maximum level at 30℃.

Optimization of Pentostatin Fermentation Conditions
ZOU Xin
2013, 3(5): 371-376. DOI: 10.3969/j.issn.2095-2341.2013.0 .
Abstract ( ) PDF (1222KB)( )

Pentostatin is an anticancer drug, mainly produced by microbial fermentation. In this study, fermentation ventilation, temperature, inoculum, seed age, and other parameters of the fermentation cycle were discussed, then a orthogonal experiment was done to determine the optimum fermentation parameters: loading volume of 60 mL, seed age of 42 h, 5% inoculum, 28℃ for 168 h fermentation, pentostatin fermentation titer reached 183 μg/mL, which was increased by 20% compared to the control.

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