Current Biotechnology

Research Progress on Transgenic Insect Resistant Cotton
Anhong ZHANG, Juanli XIAO, Zhansheng ZHAO, Zhian WANG, Yuan LIU, Xiaoli LUO
2023, 13(5): 657-662. DOI: 10.19586/j.2095-2341.2023.0019
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Cotton is one of the important cash crop in China， and insect pests have a great impact on cotton production. Breeding insect resistant cotton by transgenic technology provides an effective method for pest control. This article reviewed the research progress of genetically modified insect resistant cotton in recent years， including transgenic cotton with Bacillus thuringiensis insecticidal crystal protein gene， RNAi transgenic insect resistant cotton， gene editing to create insect resistant cotton and research on the relationship between transgenic secondary metabolite genes and cotton insect resistance. The paper was expected to provide direction for further research on transgenic insect resistant cotton， and put forward prospects for the research of transgenic insect resistant cotton.

Impact of the Fourth Modifications of Patent Laws on Biotechnology Patent Applications and Industry Development
Kehao CAO, Junli ZHU, Huashan HE, Weizhuo XU
2023, 13(5): 663-670. DOI: 10.19586/j.2095-2341.2023.0065
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Since 2020， Chinese government amended Patent Laws， Patent Law Rules and Patent Examining Guidelines for the fourth time， in order to shift Chinese economic development pattern and enhance intellectual property protection. The impact on protected topic of biological patent application and strategy of patent application were investigated by analyzing modifications of patent legislation， thereby prognosticating the impact on biological breeding （such as gene editing） industry development， bioartificial intelligence technology industry development， and biomarker medical diagnostic industry development. The results of study were intended to provide suggestion for scientists on how to formulate reasonable patent application strategies in accordance with amended patent laws， and for innovation group on how to protect innovative outcomes of biotechnology and optimizing industrial layouts in accordance with amended patent laws.

Application of Machine Learning in Phenotypic Prediction of Gut Microbiota
Haitao CAO, Jing ZHU, Yunpeng MA, Xinghua CUI
2023, 13(5): 671-680. DOI: 10.19586/j.2095-2341.2021.0201
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With the development of second-generation DNA sequencing technology， a large amount of gut microbiota data has been accumulated. The studies showed that gut microbiota were closely related to the health status of the host. Therefore how to model and analyze the complex and high-dimensional gut microbiota data has been an important challenge faced by bioinformatics at present. The rise of artificial intelligence had made it possible to process gut microbiota data and revealed the complex relationship between gut microbiota and host phenotypes. The paper summarized the present stage of gut microbiota and phenotypic correlation study among five kinds of machine learning algorithm （commonly used linear regression， support vector machine （SVM）， K-nearest neighbor， random forests and artificial neural network）， introduced five kinds of machine learning algorithms of theory and application in the related research， and to choose what kind of machine learning algorithms to predict recommendations to host phenotype. Finally， the future development of this field was prospected to provide a reference for predicting host phenotypes using machine learning based on gut microbiota data.

Research Progress on the Immune Regulation of EBV on the Host
Xujuan ZHANG, Pengxiang ZHAO, Ziyi LIU, Zisong CAI, Mengyu LIU, Fei XIE, Xuemei MA
2023, 13(5): 681-689. DOI: 10.19586/j.2095-2341.2023.0048
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As a virus with a human infection rate of up to 90%， EBV can establish long-term infection in the human body through the immune regulation of the host. The occurrence and development of many tumors are closely related to the regulation of host immunity by EBV， but the mechanism of immune regulation by EBV has not been fully elucidated. EBV has developed a series of strategies for host immune regulation in different infection states， mainly by targeting Toll like receptors（TLRs） signaling pathways， using or limiting part of their immune effects to promote the establishment of long-term infection and evade host immune surveillance to maintain the stability of the EBV genome. This article reviewed the immunoregulatory mechanism of EBV， a double-stranded DNA herpesvirus， in order to provide basic theoretical basis and research ideas for the prevention and treatment of virus infection and virus-associated tumors.

Application of Anti-idiotype Antibodies in Haptens Immunoassay
Kai DU, Zhuoling ZHANG, Li GAO, Haihua HE, Tinghua LI, Wei RAO
2023, 13(5): 690-697. DOI: 10.19586/j.2095-2341.2023.0066
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The detection of small molecule haptens is of great significance for food and drug testing， environmental monitoring and disease diagnosis. However， commercially available immunoassays for haptens are limited， partially due to poor performance， such as low sensitivity. In the early 20th century， anti-idiotypes （Ab2） were discovered， and Ab2 can be divided into three different isoforms： Ab2α， Ab2β and Ab2γ. In addition to be used for the treatment and prevention of disease， Ab2 has potential applications in immunoassays. This review provided an overview of the use of Ab2 in competitive， non-competitive and phage-based immunoassays， including the different detection formats and effects on the performance. Both the use of appropriate Ab2 instead of hapten derivatives in competitive assays and the use of anti-metatype antibodies against primary antibody and hapten complexes in non-competitive assays could increase detection sensitivity and improve cross-reactivity. The use of Ab2 could be a potentially important choice for the development of in vitro diagnostic assays in the future. In addition， different methods for the generation of Ab2 were summarized. The selection of natural nanobody libraries might be a more practical way to obtain Ab2 than traditional monoclonal antibody production.

Effects of Different Sources of CHO Host Cells on Antibody Expression
Hui CAO, Jing DONG, Yu JIA, Yifan JIANG
2023, 13(5): 698-703. DOI: 10.19586/j.2095-2341.2023.0064
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CHO cells comprise a variety of lineages including CHO-K1， CHO-DG44 and CHO-S， which have been widely used in the industrial production of biological drugs. All CHO cell lines share a common ancestor， however， during the process of cell passage cultivation， cell domesticated， and preservation by different laboratories or companies， substantial genetic heterogeneity among them has been produced， that showed great differences in cell growth state， antibody titer， glycosylation and other product quality attributes. This article reviewed the difference in chromosome， growing status and expression， and glycoform in different sources of CHO host cells， which was expected to be helpful in host cell selection during antibody drug research and development process.

Pro-oncogenic Function and Therapeutic Significance of SALL4
Linlin DU, Fei XIE, Xuemei MA
2023, 13(5): 704-711. DOI: 10.19586/j.2095-2341.2023.0049
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Spalt-like gene 4 （SALL4）， a new member of the SALL transcription factor family discovered in 2002， plays a crucial role in maintaining self-renewal and pluripotent embryonic stem cells （ESCs）. SALL4 is specifically expressed in embryonic stem cells and germ cells， but is down-regulated or silenced in most adult cells. However， recent studies have shown that SALL4 is re-expressed in leukemia， breast cancer， stomach cancer， ovarian cancer， liver cancer， lung cancer， melanoma and other tumors， showing its characteristics of proto-oncogene， and the expression level of SALL4 is directly correlated to tumor progression， poor prognosis and survival of patients. The re-expression of SALL4 is mediated by a variety of cytokines， and acts as a transcription factor to regulate expressions of downstream gene and signaling pathways to promote tumor occurrence， metastasis， metabolism and drug resistance. Targeted inhibition of SALL4 expression and biological function shows obvious antitumor effect.SALL4 has become a new tumor marker and potential therapeutic target due to its properties of re-expression in tumor tissues and promoting tumor occurrence and development. The article summerized the structure and function of SALL4 protein， and its molecular mechanism of activation expression. This article focused on the mechanism and diagnostic value of SALL4 molecule in the development of tumor and the significance of targeted therapy， which was expected to provide reference data for cancer therapy.

Advances on the Role of Piezo1 in Cancer
Yuemaierabola ANWAIER, Lili SUN, Yeerkenbieke BUERLAN, Wenjia GUO
2023, 13(5): 712-717. DOI: 10.19586/j.2095-2341.2023.0016
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Piezo1 is a family member of mechanically sensitive piezoelectric ion channels that convert various mechanical stimuli into electrochemical signals. Piezo1 plays an important role in several malignant tumors as a carcinogenic medium， mediating the proliferation， migration and invasion of various cancer cells through various mechanisms. Several studies have linked the expression of Piezo1 to the clinical characteristics of aging and cancer patients， making Piezo1 promising as a new biomarker for the diagnosis and prognosis of several human cancers. This paper summarized the structure of Piezo1， dynamic characteristics of the Piezo1 channel and its function in cancer， which was expected to provide reference for Piezo1 used in diagnosis and prognosis of cancer and tumor biomarker and therapeutic target.

Cell Death in Cisplatin-induced Kidney Injury
Mingjiao ZHANG, Jiefu ZHU, Xiongfei WU
2023, 13(5): 718-724. DOI: 10.19586/j.2095-2341.2023.0036
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As a non-specific drug that interferes with cell cycle， cisplatin is widely used in clinical anti-tumor therapy. However， cisplatin can induce renal cell death and impair renal function. Regulated cell death （RCD） refers to a process of well-controlled programmed cell death with well-defined pathological mechanisms. In recent years， there have been many studies on the cell death modes related to the pathogenesis of various types of acute kidney injury（AKI）， but the role and mechanism of cell death in cisplatin-induced kidney injury remains vacant. Therefore， a comprehensive understanding of the mechanism of cell death in cisplatin-induced nephrotoxicity may provide new therapeutic targets for improving cisplatin-associated nephropathy. This paper summarized the cell death mode in cisplatin-induced kidney injury， including apoptosis， necrotic apoptosis， pyroptosis and ferroptosis， which was expected to provide a possible scheme for the use of cisplatin in tumors therapy with less renal injury.

Research Advances of POSTN in Cardiovascular Diseases
Xuesheng FANG, Mingwei BAO
2023, 13(5): 725-729. DOI: 10.19586/j.2095-2341.2023.0085
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Cardiovascular disease is the leading cause of death from chronic diseases worldwide， and disease prevention and treatment have brought a heavy burden to the country. Periostin （POSTN） is an extracellular matrix related protein that involved in various biological processes such as cell proliferation， migration， differentiation and inflammatory response. Recent studies have shown that POSTN is widely implicated in the occurrence and development of cardiovascular diseases， such as atherosclerosis， vascular calcification， cardiac remodeling after myocardial infarction， pulmonary hypertension， valvular heart disease， etc. This article reviewed the research advances of POSTN in cardiovascular diseases， and provided new ideas for early detection， early diagnosis， early treatment and prognostic evaluation of cardiovascular diseases with POSTN as the target.

Development of InDel Molecular Markers in Sorghum Using Re-sequencing Technology
Weijun PU, Binglan TAN, Danchen HE, Pan ZHANG, Yubin LI, Li ZHU
2023, 13(5): 730-741. DOI: 10.19586/j.2095-2341.2023.0106
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Sorghum （Sorghum bicolor L. Moench） is one of the most important cereal， and the complete genome of sorghum sequencing variety BTx623 has been published， which provides a research basis for the development of InDel（insertion/deletion） markers in sorghum. InDel markers， as high-throughput molecular markers， have the advantages of high genetic stability， wide distribution， strong polymorphism， and strong universality in plant genomes. At present， it has been widely used in rice， corn， cotton and other major crops. However， there are few studies in sorghum. In this study， the genomic DNA of sweet sorghum variety JIUTIAN1 was selected as the research object， re-sequencing was performed using Illumina platform， and quality control was performed on raw data of sequencing using Trimmomatic software， and low-quality data were eliminated. BWA software was used to compare the obtained high-quality clean data with the reference genome of the sequenced variety BTx623， from which a large number of InDel sites were detected. These InDel sites were used to design 205 evenly distributed markers in the whole genome， and 87 polymorphic markers were selected by PCR verification， with a polymorphism of 42.44%. These 87 InDel markers were characterized with high polymorphism， wide distribution and high stability. The sorghum InDel marker developed in this study was expected to lay a foundation for the construction of sorghum genetic linkage map， genetic diversity analysis， hybrid purity identification， location of genes related to important agronomic traits of sorghum， and marker-assisted selection breeding.

Effects of Exogenous Phytohormones on Heat Tolerance of Epipremnum aureum Under Hydroponic Culture
Zhonghui CHEN, Jinghong LI
2023, 13(5): 742-747. DOI: 10.19586/j.2095-2341.2023.0083
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In the summer， high temperature stress susceptible to Epipremnum aureum， causing stunted plant growth. The effects of different concentrations of abscisic acid， paclobutrazol and uniconazole on hydroponic Epipremnum aureum heat resistance were studied in this research. The results showed that， under high temperature stress at 35 ℃， exogenous plant hormones could improve the heat resistance of Epipremnum aureum to a certain extent， and the treatment of 2.5 mg·L^-1 abscisic acid and 2.0 mg·L^-1 uniconazole had the best effects. The results indicated that in high temperature areas or seasons， a certain concentration of plant hormones can be used to improve the heat resistance of Epipremnum aureum and ensure their long-lasting ornamental value. This study provided theoretical and practical guidance on how to improve the stress resistance of hydroponic Epipremnum aureum.

Adaptive Evolution Analysis of Mitochondrial Genomes in Anseriform Birds with Various Feeding Habits
Weiping ZHANG, Bingying QIU, Dongsheng ZHANG
2023, 13(5): 748-754. DOI: 10.19586/j.2095-2341.2023.0054
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Previous studies showed that， animals' feeding habits are related to their energy metabolism and adaptation. Energy metabolism takes place in mitochondria， and 13 subunits of the oxidative phosphorylation complexes were encoded by mitochondrial genome. According to feeding habits， birds of Anteriformes could be divided into three groups， including carnivorous， herbivorous and omnivorous. To study the evolutionary effects of feeding habit on mitogenomes， we selected 20 anteriformes species and divided them into three groups. We downloaded their mitogenomes， and did adaptive analysis， relax analysis， polymorphism analysis and 3D structure prediction. Our results showed that， the carnivorous group had higher evolutionary rates than the other two groups， and only the carnivorous group had multiple genes under relaxed selection. Meanwhile， the carnivorous group had more polymorphism sites and deterious sites among their proteins sequences than the other two groups. The genes from the omnivorous group showed decreased evolutionary rates， and most of the genes were under intensification selection， furthermore， less polymorphic sites were detected in this group. The results indicated that the proteins encoded by mitochondrial genomes of anteriformes birds with different feeding habits are subject to different selective pressures. This study provides a molecular basis for the influence of dietary differences on the adaptive evolution of avian mitochondrial genomes.

Safety Evaluation of Clostridium butyricum Based on Whole Genome Sequencing
Yingrui GAO, Fuzhong KANG, Tiejian MENG, Kefei LIU, Tiaotiao WANG, Jinyan CHEN, Tong SUN
2023, 13(5): 755-759. DOI: 10.19586/j.2095-2341.2023.0075
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Clostridium butyricum is a specialized anaerobic bacterium with Gram positive. Its main metabolites include not only butyric acid， but also organic acids， such as lactic acid and acetic acid. As a probiotic， clostridium butyricum is widely distributed in the intesines of animals and humans， and has shown good effects in disease prevention and treatment of enteritis and other diseases. Conducting whole genome sequencing of Clostridium butyricum to analyze its resistance， virulence genes and pathogenicity， which comprehensively evaluate its safety performance. The results showed that the total length of the genomic DNA of Clostridium butyricum was 4 709 567 bp， including a circular chromosomal DNA， a circular plasmid and two linear plasmid DNA. The size of circular chromosome DNA is 3 873 131 bp， the molar percentage of G+C is 28.86%， the size of circular plasmid DNA is 769 297 bp， the G+C content was 28.33%， the size of linear plasmid 1 DNA was 49 922 bp， the G+C content was 28.26%， the size of linear plasmid 2 DNA was 17 217 bp， and the G+C content was 27.89%. A total of 4 285 genes， 87 tRNA genes， 44 rRNA genes， and 3 sRNA genes were detected in the genome. Compared with CARD and ARDB databases， no drug resistance genes and pathogenicity related genes included in the database were detected in the whole genome of Clostridium butyricum， which laid the foundation for its future safety evaluation tests.

Construction and Characterization of Plant-based Egg Liquid System
Lanyi ZHI, Zhe LIU, Qiang WANG, Aimin SHI
2023, 13(5): 760-770. DOI: 10.19586/j.2095-2341.2023.0060
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Using mung bean protein （MBP）， curdlan and soybean oil as raw materials， plant-based egg liquid was constructed， and the effects of transglutaminase （TGase）， CaCl₂ and soybean lecithin （SL） on the plant-based egg liquid and its emulsion gels were systematically explored. The properties of the emulsions were studied by particle size， confocal laser scanning microscope （CLSM） and apparent viscosity， and the properties of the emulsion gels were characterized systematically by texture， water holding capacity （WHC） and scanning electron microscope （SEM）. The results showed that when the amount of TGase added was 0.7%， the emulsion particle size was the smallest at 34.67±1.85 μm， while the addition of 0.2% CaCl₂ resulted in the largest particle size at 46.63±0.47 μm. The good emulsifying property of SL caused the droplets to continuously decrease after adding it to the system. The apparent viscosity of the emulsion decreased with the increase of TGase and CaCl₂， and increased with the increase of SL. When 0.3% TG enzyme was added， the emulsion gel had the highest hardness and WHC （231.58 ±10.94 g， 43.39%±0.90%）， while the emulsion gel had the highest hardness （125.37±8.03 g） and the lowest WHC （34.26%±0.46%） when 0.15% CaCl₂ was added. The addition of SL weakened the texture of the emulsion gel. The results of this study demonstrated that the characteristics of plant-based egg liquid and its emulsion gel can be adjusted by TGase， CaCl₂ and SL， which has important guiding significance for improving the quality of plant-based egg liquid and achieving industrialization.

Preparation Technology and Characterization of Lipase Immobilized with Acetylated Arachin Nanoparticles
Wei LI, Aimin SHI, Bo JIAO, Qiang WANG
2023, 13(5): 771-778. DOI: 10.19586/j.2095-2341.2023.0051
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Lipases are currently commonly used in the food industry for the modification of oils and fats， such as esterification and transesterification. In order to improve the chemical stability， thermal stability， and reusability of lipases， glutaraldehyde （GDA） was used as a crosslinking agent to crosslink lipase with acetylated arachin particles （AAPs）. And the immobilization efficiency and specific activity of protein carrier enzyme particles （lipase-AAPs） were optimized. The results showed that when the addition amount of succinic anhydride was 30% of the dry base mass of protein （the degree of acetylation was 41.71%±0.01%）， the GDA concentration was 1.0%， the crosslinking time was 1 h， the lipase concentration was 10%， and the lipase to particle ratio was 1∶4， the optimal immobilization efficiency and specific activity of lipase-AAPs were 12.95%±0.03% and 1.74±0.07 U·mg^-1， respectively. Micromorphological observation of lipase before and after crosslinking was performed using scanning electron microscopy （SEM）. Characterization of interactions between protein and lipases was measured by fourier transform infrared spectroscopy （FTIR）. In this paper， a method for the preparation of protein-loaded enzyme particles was provided， which was expected to provide theoretical and technical support for natural organic macromolecules as lipase carriers and avoid the safety problems caused by inorganic and organic synthetic particles.

Quantitative Determination of Fucoidan by Improved 1,9-dimethyl-methylene Blue Method
Weiping ZHAO, Yihui QI, Jingjing LI, Shuang SONG, Rui ZHAI, Qianqian DU
2023, 13(5): 779-784. DOI: 10.19586/j.2095-2341.2023.0046
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As a kind of sulfated polysaccharide mainly extracted from sea cucumbers and brown algae， etc.， fucoidan has anticoagulant， antitumor， antithrombotic and other biological activities， and has broad application prospects in the pharmaceutical and food industries. Currently， 1，9-dimethylmethylene blue （DMMB） colorimetry is one of the common methods for quantitative detection of fucoidan， but there are some problems such as poor sensitivity， cumbersome operation and long time consumption. This study optimized the concentration， pH， and reaction time of the DMMB method， and significantly improved the linear range， precision， recovery rate and limit of quantification of the improved detection method. The optimal reaction conditions for the improved DMMB method to detect fucoidan were a DMMB solution concentration of 42.8 mg·mL^-1, pH 3.3, a dark reaction, and detection between 10~15 minutes. The methodological investigation results showed that the improved DMMB colorimetric method raised the sensitivity of the quantitative detection method for fucoidan， expanded the detection concentration range to 0.012 5~0.300 0 mg·mL^-1， and provided a fast and effective means for the quantitative analysis of fucoidan， which was conducive to the development and utilization of fucoidan.

Transfection and Inhibition of Tumor Cells by Survivin ASO Mediated by Graphene-shelled Ferro-nitride Magnetic Beads
Xiaoxu ZHANG, Xiangqian XIAO, Yiqun PAN, Yexiang GU, Li DONG, Haoran DANG, Xi KANG, Minglian WANG
2023, 13(5): 785-797. DOI: 10.19586/j.2095-2341.2023.0058
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Based on the biocompatibility and excel affinity for single-strand nucleic acids of graphene， we investigated the transfection ability of graphene-shelled ferro-nitride magnetic beads （GFeNMB） carrying the antisense oligonucleotides （ASO） to target Survivin， and the inhibitory effect on tumor cells. Firstly， the ASO sequences were designed based on the secondary structure of Survivin mRNA predicted by RNA Draw software， and FAM-labeled and unlabeled ASOs were synthesized. The affinity of GFeNMB for single-strand nucleic acids was detected based on fluorescence with FAM-ASO. Based on the adsorbability of graphene to single-stranded nucleic acid and the quenchability of fluorophore， the fluorescence intensity detection method was used to detect the adsorption capacity of GFeNMB to ASO， and GFeNMB and GFeNMB-ASO were characterized with transmission electron microscope and laser granulometer. Survivin ASO was magnetically transfected into human non-small cell lung cancer cell A549 with a magnetic pole. The changes of Survivin expression were tested by Western blot， apoptosis by reactive oxygen species （ROS） kit and flow cytometry， cell proliferation and migration ability by CCK-8 assay and scratch assay. The results confirmed the excel affinity of GFeNMB for ASO， and the maximum adsorption amount （0.44 μg·mg^-1） was reached after 20 min of mixing GFeNMB with ASO. FAM-ASO was magnetically transfected into cells， which was confirmed by intracellular fluorescence focused on the nucleus observed under confocal microscope. Moreover， GFeNMB mediated transfection into nucleus was significantly superior to the liposome transfection. The down-regulated level of Survivin protein expression after magnetic loading ASO was better than those of the untreated and liposome transfected groups， followed by an increase in the proportion of apoptotic cells， an increase of intracellular ROS level， and inhibition of cell proliferation and migration. The results suggested that GFeNMB could transfect Survivin ASO into tumor cells， thus inhibit tumor cell proliferation and induce apoptosis. The down-regulation of gene expression after GFeNMB-ASO magnetofection of cells also indicated that GFeNMB may be a useful transfection medium for single-strand oligonucleotides.

Research on Feature Selection of Gut Microbiota and Disease Prediction Model Based on Weighted Average
Haitao CAO, Jing ZHU, Haibo ZENG, Yanchen LIU
2023, 13(5): 798-806. DOI: 10.19586/j.2095-2341.2023.0063
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The utilization of metagenomic analysis to investigate human diseases and predict health conditions is a current focal point of research. Through the application of bioinformatics tools such as KneadData and MetaPhlAn2， the raw metagenomic data undergoes quality control and host contamination removal were carried out to obtain the pure sequences. Subsequently， dimensionality reduction methods and a random forest model were employed to identify microbial taxa that were highly correlated with disease occurrence， serving as replacements for the original data features in the disease prediction model. A fusion disease prediction model was constructed by integrating multilayer perceptron （MLP）， support vector machine （SVM）， and extreme gradient boosting （XGBoost） as sub-models. Following feature selection and cross-validation on datasets pertaining to liver cirrhosis， type 2 diabetes， and obesity， the obtained AUC values were 0.928 6， 0.652 1， and 0.574 7， respectively. The area under the ROC curve demonstrated that the model augmented with the selected microbial taxa， which could efficiently and accurately screen and diagnose diseases， effectively distinguishing between healthy individuals and patients. This work provided valuable insights for the establishment of a novel non-invasive and quantifiable auxiliary diagnostic method.

Research on the Mechanism of Jinyinhua Oral Liquid in the Prevention and Treatment of COVID-19 Based on Network Pharmacology and Molecular Docking
Changhu KE, Yaqing WU, Xueru DING, Huimin HUANG, Hui YAN
2023, 13(5): 807-817. DOI: 10.19586/j.2095-2341.2023.0052
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To predict the mechanism of Jinyinhua oral liquid in the prevention and treatment of corona virus disease 2019 （COVID-19） based on network pharmacology and molecular docking. The chemical ingredients of drugs were collected by traditional Chinese medicine systems pharmacology database and analysis platform （TCMSP）， while Swiss Target Prediction were used to predict the corresponding targets of ingredients. The targets of COVID-19 were retrieved in GeneCards， CTD， TTD and DrugBank database. Venny 2.1.0 online software was used to obtain the common targets of drugs-disease， and then the “drug-compound-target-disease” network diagram was constructed by using the software Cytoscape 3.8.2. The String database was used to draw the protein-protein interaetion network， and the perform gene ontology （GO） function and the target kyoto encyclopedia of genes and genomes （KEGG） pathway enrichment analysis were carried out through David database， and the AutoDock platform was applied in verifying the molecular docking of components and targets. The results showed that the 16 active components of Jinyinhua oral liquid could regulate 88 targets and 108 pathways to treat COVID-19. 5-Hydroxy-7-methoxy-2-（3，4，5-trimethoxyphenyl）chromone， chryseriol， luteolin， mandenol， kaempferol， quercetin and other active compounds， which could mediate HIF-1， endocrine resistanc， PI3K-Akt， VEGF， relaxin， ErbB， hepatitis C， hepatitis B， Rap1 and other signaling pathways through AKT1， SRC， EGFR， HIF1A， PPARG， MMP9， ESR1， MAPK1， PTGS2， MMP2 and other key target proteins. The result of molecular docking showed that the targets and the components had a certain degree of binding. Jinyinhua oral liquid can participate in the prevention and treatment of COVID-19 through multiple components， targets and pathways， which provided a theoretical basis for the clinical application of Jinyinhua oral liquid and the mechanism of its prevention and treatment of COVID-19.

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