Table of Content

• 
• Current Biotechnology
  Volume 5 Issue 5 25 September 2015
• Progress of Aseptic Purification Technique of Cyanobacteria
• LI Tian-li, ZHENG Ling-ling, ZHANG Qi, SONG Li-rong*
• 2015, 5(5):  329-334.  DOI: 10.3969/j.issn.2095-2341.2015.05.01
• Abstract ( ) PDF (1199KB)( )
With the vigorous development of the microalgae industry, people are paying much more attention to cyanobacteria according to its important economic value, as its polysaccharide and protein are applied in many fields. In order to excavate the characteristics and function of cyanobacteria more in\|depth and clearly, it is necessary to obtain axenic cultures of cyanobacteria, which is also regarded as the foundation for the further research on physiological, biochemical, genetic and taxonomic characteristics of cyanobacteria. This paper mainly reviewed the chosen of appropriate aseptic purification methods based on their structures and physiological characteristics. Furthermore, the methods based on the differences between cyanobacteria and contaminated bacteria were introduced. The paper was expected to provide useful information for the works on purification of cyanobacteria.
• The Extraction and Application Status of Proanthocyanidins from Grape Seed
• LI Dou, WANG Shan-shan*
• 2015, 5(5):  335-339.  DOI: 10.3969/j.issn.2095-2341.2015.05.02
• Abstract ( ) PDF (1154KB)( )
Currently, proanthocyanidins  is internationally recognized as the most effective natural antioxidants which can scavenge free radicals in the body. Proanthocyanidins is widely distributed in natural plants. This paper described the function of proanthocyanidins in the grape waste, and analyzed its application development status. Combined with common extraction method, and the progress of proanthocyanidins at home and abroad, the process parameters of extracting the anthocyanin from grape seed was optimized, and the best extraction method of proanthocyanidins from grape seeds was get. The paper was expected to provide scientific basis for industrialized production and comprehensive utilization of proanthocyanidins from grape seed, so as to the  proanthocyanidins has a wider application.
• Comprehensive Research and Utilization of Functional Components in Quinoa
• DING Yun-shuang1, ZENG Ya-wen2*, MIN Kang3, YI Bin3
• 2015, 5(5):  340-346.  DOI: 10.3969/j.issn.2095-2341.2015.05.03
• Abstract ( ) PDF (1234KB)( )
Quinoa (Chenopodium quinoa Willd) is an annual herb of Chenopodium genus in the Chenopodiaceae familia, which attracts the attention of the researchers for its unique nutritional value and potential health functions. This article summarized the research progress for growth, nutritive value, functional components, gene analysis and physiological function of quinoa. According to the progress of comprehensive research and utilization of functional components in Quinoa at home and abroad, we put forward to countermeasures of germplasm innovation, health food, pharmaceutical research, new functional food development and industrialization for quinoa, which had important reference value for quinoa industry, health food, pharmaceutical development and other fields.
• Progress on Function Reseach of IL-6 in Metabolic Diseases and Cancer
• ZHANG Shuang1,2, ZHENG Dong2, MA Yue-hui1*
• 2015, 5(5):  347-350.  DOI: 10.3969/j.issn.2095-2341.2015.05.04
• Abstract ( ) PDF (1148KB)( )
Interleukin IL-6 is widely exist in inflammatory, and it is frequently viewed as a proinflammatory cytokine. IL-6 has parallel functions with tumor necrosis factor (TNF) and IL-1β in the context of inflammation. IL-6 can stimulate activation of B cell proliferation and secrete antibody, can stimulate T cell proliferation and CTL activation, can stimulate synthesis acute phase proteins of the liver cells and participate in inflammation, can promote the blood cells development. However, accumulating evidence points to a broader role of IL-6 in a variety of (patho)physiological conditions, including functions related to the resolution of inflammation. This paper reviewed recent findings on the complex biological functions governed by IL-6 signaling, focused on its role in inflammation-associated cancer and metabolic disorders such as obesity, liver metabolism, skeletal muscle metabolism and exercise. Finally, the paper outlined important areas of inquiry towards understanding this pleiotropic cytokine, which was expected to provide reference for IL-6 application in disease treatment.
• High Temperature Stress Effect on Main Quality Characters of Rice in Heading and Flowering Period
• MA Ting-chen, XIA Jia-fa, WANG Yuan-lei, ZHOU Kun-neng, LI Ze-fu*
• 2015, 5(5):  351-358.  DOI: 10.3969/j.issn.2095-2341.2015.05.05
• Abstract ( ) PDF (4205KB)( )
Great losses to the rice production are caused by high temperature stress. Using the heat resistant materials screened by International Rice Research Institute, we studied the high temperature stress effect on grain quality of rice in heading and flowering period, the results were as follows: There were big differences of heat tolerance among the heat resistant materials screened by International Rice Research Institute, and there were some materials relatively sensitive to high temperature; high temperature stress greatly affected quality evaluation indexes of rice in heading and flowering period; the changes of rice transparency, length-width ratio, chalky rate, gelatinization temperature, protein content were not related to heat resisting property of rice varieties; under high temperature stress, there was a significant negative correlation between heat resistant coefficient of rice varieties and the change of gel consistency, the correlation coefficient was -0.516 70, and there was a significant positive correlation between the change of amylase content  and heat resistant coefficient of rice varieties, the correlation coefficient was 0.659 78.
• The Promoter Cloning and Activity Analysis of Maize bHLH Transcription Factor ABP7  
• WANG Yin-xiao, ZHAO Jun*
• 2015, 5(5):  359-365.  DOI: 10.3969/j.issn.2095-2341.2015.05.06
• Abstract ( ) PDF (2007KB)( )
Basichelix-loop-helix (bHLH) transcription factors are widely present in plants, which play important roles in flower morphogenesis, light signal transduction and hormonal signal transduction. However, the molecular mechanisms regulating their expression remain largely unknown. In this paper, we previously cloned a bHLH transcription factor gene from maize, designated ABP7, and preliminary data suggestted that it might be involved in multiple signal transduction pathways such as kernel development and hormone response. For further elucidation of the upstream pathways regulating ABP7 expression, we cloned the 5′ flanking sequence of ABP7, verified its promoter activity by transient expression in maize protoplasts, and predicted its cis-acting elements. The results showed that the cloned 2 266 bp 5 ′ flanking region is a functional promoter which is capable to drive the expression of downstream reporter, and contains many potential cis-acting elements involved in the signaling of hormones such as GA、 IAA and ABA, and responses to adverse environments. These results set a base for future studies in identification of upstream regulatory networks that control ABP7 expression, and in unraveling the specific roles of ABP7 in plant growth, development and stress response.
• Cloning and Sequence Analysis of GhRACK1 Gene Dominant Expressed in Fiber from Gossypium hirsutum
• PANG Wei-min, JIN Xi, WANG Xu-jing, YANG Jiang-tao, LV Shao-pu, TANG Qiao-ling, WANG Zhi-xing*
• 2015, 5(5):  366-370.  DOI: 10.3969/j.issn.2095-2341.2015.05.07
• Abstract ( ) PDF (1944KB)( )
Fiber quality improvement is one of the main targets in cotton breeding. The discovery of specific or dominant expressed gene  in fiber is the key factor that improved fiber quality by using genetic engineering strategy. In this study, we used G. hirsutum var. Sumian12 as a starting material to clone the full-length cDNA of GhRACK1 gene by RACE techniques according known EST sequence. The deduced amino acid sequence of GhRACK1 was highly homologous to the other known RACK1 proteins and contained 4 cascade WD motifs, which belonged to WD40 family. GhRACK1 protein and known RACE1 had similar three-dimensional structure by PDB simulation. qPCR results indicated that the expression level of GhRACK1 in fiber was more than 20-fold compared with leaf. This study provided new gene resource for cotton fiber improvement.
• Effects of Photosynthetic Bacteria Inoculants on Soil Microorganisms
• YANG Pan-pan, ZHANG Hai-chun, LIU Li-hui, PU Qiang, PENG Gui-xiang*
• 2015, 5(5):  371-376.  DOI: 10.3969/j.issn.2095-2341.2015.05.08
• Abstract ( ) PDF (2439KB)( )
In order to select a suitable photosynthetic bacteria inoculants concentration to improve the tomato growth with pot experiment, we studied the effects of three concentrations of high efficient photosynthetic bacteria inoculants on soil microbial properties. The experimental results showed that, significant increases was observed in the population of soil bacteria and soil Actinomycetes with photosynthetic bacteria inoculants, and prominent decrease in that of soil fungi. Soil urease, acid phosphatase and catalase activities were all improved by the application of photosynthetic bacterial inoculants. The photosynthetic bacterial inoculants diluted 100 times plus chemical treatment was the most remarkable in the experiment, which was more conducive to the soil microbes and environment.  
• Screening of Actinomycin D  High-producing Strain by Streptomycin Resistant Mutant Selection Coupled with Microwave Mutagenesis
• REN Lin-ying, ZHANG Zhu-lan*, WANG De-sen, YANG Huang-jian, TANG Wen-li, SUN Fei
• 2015, 5(5):  377-381.  DOI: 10.3969/j.issn.2095-2341.2015.05.09
• Abstract ( ) PDF (2406KB)( )
Microwave mutagenesis and streptomycin resistance screening was applied to screen  actinomycin D high-producting strain. After testing the resistance of Streptomyce rubiginosohelvolus FIM-N31  to streptomycin, the lethal concentration of streptomycin was determined. A lot of streptomycin-resistant mutants were screened after the spores regenerated on the lethal streptomycin concentration (50 μg/mL) media when they were treated with microwave irradiation. It was eventually found that the high-yield strain Str186 from these mutants which was stably inheritable, and the productivity was beyond eight times higher than the original strains in the rotation-flask experiments.
• Study on an Efficient Medium to Culture the Nematophagous Fungus Drechmeria coniospora
• GUO Qian-nan, ZHOU Zheng-fu, XU Yu-quan, ZHANG Wei*
• 2015, 5(5):  382-386.  DOI: 10.3969/j.issn.2095-2341.2015.05.10
• Abstract ( ) PDF (2081KB)( )
There is an obstacle to doing scientific research on nematophagous fungus Drechmeria coniospora, owing to the shortest growth cycle of the microbe in conventional medium (including PDA, SDAY and CMA) is 13 days at least.  In this study, we utilized LK-100 medium to culture D. coniospora at room temperature, and observed its growth status under microscope. The results showed that growth cycle of D. coniospora on LK-100 medium was only 8 days. Furthermore, the infection efficiency of conidia, obtained from LK-100 medium, to natural host Caenorhabditis elegans was higher than that obtained from conventional fungi media. LK-100 medium, a kind of effective medium, will contribute to the further study of D. coniospora.
• Isolation and Identification of a Bacterium Tolerant to High Concentration Heavy Metals
• ZENG Qi-yu, LIANG Li-ni, YANG Yong-feng, ZHU Mei-juan, SU Ping*
• 2015, 5(5):  387-391.  DOI: 10.3969/j.issn.2095-2341.2015.05.11
• Abstract ( ) PDF (2411KB)( )
A strain that resist to heavy metals with high concentration，named ZM-12, was isolated by using selective culture medium containing different concentrations of heavy metals. It was identified as Enterobacter according to the BLAST comparison, the analysis in MEGA 4.0 and the phylogenetic tree. ZM-12 was Gram-negative by using the Gram stain test. In different heavy metal medium, the MIC value is different. It can be tolerant of Cu2+ 22.33 mmol/L, Ni2+ 58.69 mmol/L, Pb2+ 17.84 mmol/L and Mn2+>200mmol/L. The removal rate of heavy metals can be obtained by atomic absorption spectroscopy (AAS). The removal rate of Pb2+, Mn2+, Cu2+, Ni2+ were 97.68%, 99.93%, 41.23%, 99.46%, respectively. We can conclude that ZM-12 has high resistance to many different heavy metals. The isolation and identification of this strain can provide some basis for the pollution caused by heavy metals.
• Progress on Application of Yeast Two-hybrid Technique
• WANG Ting, GE Huai-Na, GUO Hong*
• 2015, 5(5):  392-396.  DOI: 10.3969/j.issn.2095-2341.2015.05.12
• Abstract ( ) PDF (1165KB)( )
Yeast two-hybrid system is one of the most powerful and widely used method to detect protein-protein interaction in living cells. Yeast two-hybrid technique is cost-effective, easy to operate, can reach the whole-genome level, and identify the interaction between different varieties. Compared with the traditional detected method, it has obvious advantages and has been applied in more and more fields. In this paper, the yeast two-hybrid technology principle and application were reviewed, including the applications on discovering new protein and proteins new function, setting up the protein linkage map, studing of human genome DNA library, screening target for drug, and so on. The paper was expected to provide reference for the application of yeast two-hybrid system.