Abstract: A strain that resist to heavy metals with high concentration，named ZM-12, was isolated by using selective culture medium containing different concentrations of heavy metals. It was identified as Enterobacter according to the BLAST comparison, the analysis in MEGA 4.0 and the phylogenetic tree. ZM-12 was Gram-negative by using the Gram stain test. In different heavy metal medium, the MIC value is different. It can be tolerant of Cu2+ 22.33 mmol/L, Ni2+ 58.69 mmol/L, Pb2+ 17.84 mmol/L and Mn2+>200mmol/L. The removal rate of heavy metals can be obtained by atomic absorption spectroscopy (AAS). The removal rate of Pb2+, Mn2+, Cu2+, Ni2+ were 97.68%, 99.93%, 41.23%, 99.46%, respectively. We can conclude that ZM-12 has high resistance to many different heavy metals. The isolation and identification of this strain can provide some basis for the pollution caused by heavy metals.

Key words: strain with heavy metals tolerance, Enterobacter, 16S rDNA, minimum inhibitory concentration (MIC), the removal rate

摘要： 利用含不同浓度的重金属选择性培养基，对采集的微生物样本进行分离，并筛选出一株超高耐受重金属菌ZM-12。对ZM-12的16S rDNA高变区序列进行BLAST比较和MEGA 4.0分析，结果通过同源进化树分析表明该菌属于肠杆菌属（Enterobacter）。对该菌进行生理生化实验、最低抑菌浓度（MIC）的测定和火焰原子吸收实验，结果表明：ZM-12属于革兰氏阴性杆菌，在不同种类的重金属培养基中，其MIC值各不相同，Cu2+、Pb2+、Mn2+、Ni2+对ZM-12的MIC值分别为22.33 mmol/L、14.48 mmol/L、>200 mmol/L、58.69 mmol/L。通过火焰原子吸收的方法，测定了ZM-12对培养基中重金属的去除率，培养一定时间后，分别测定了溶液中重金属Pb2+、Mn2+、Cu2+、Ni2+的浓度，得到其最大去除率分别达到97.68%、99.93%、41.23%、99.46%。该菌株的发现及鉴定为重金属污染环境的治理提供了参考。

关键词: 重金属耐受菌, 肠杆菌属, 16S rDNA, 最低抑菌浓度（MIC）, 去除率