Abstract: There is an obstacle to doing scientific research on nematophagous fungus Drechmeria coniospora, owing to the shortest growth cycle of the microbe in conventional medium (including PDA, SDAY and CMA) is 13 days at least.  In this study, we utilized LK-100 medium to culture D. coniospora at room temperature, and observed its growth status under microscope. The results showed that growth cycle of D. coniospora on LK-100 medium was only 8 days. Furthermore, the infection efficiency of conidia, obtained from LK-100 medium, to natural host Caenorhabditis elegans was higher than that obtained from conventional fungi media. LK-100 medium, a kind of effective medium, will contribute to the further study of D. coniospora.

摘要： 作为一种内寄生食线虫真菌，圆锥掘氏梅里霉（Drechmeria coniospora）在常规真菌培养基（如PDA、SDAY和CMA）中生长周期较长（最短为13 d），不利于对其进行科学研究。应用LK-100固体培养基常温培养D. coniospora，通过显微镜观察其生长状态，确定生长周期。研究表明，D. coniospora在LK-100培养基中的生长周期仅8 d，远短于在常规培养基上的生长周期。此外，与常规培养基相比，该培养基获得的D. coniospora分生孢子具有更强的侵染宿主秀丽隐杆线虫（Caenorhabditis elegans）的能力。LK-100培养基为进行D. coniospora相关研究提供了快速、高效的培养方法。

关键词: 圆锥掘氏梅里霉, LK培养基, 生长周期, 侵染能力