Curr. Biotech. ›› 2013, Vol. 3 ›› Issue (4): 281-287.DOI: 10.3969/j.issn.2095-2341.2013.04.10

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Isolation of Bacteria in the Saline Alkaline Soils of Akesu in Xinjiang

LI Liang-qiu, ZHEN He-yun, CAI Zhuo-ping, YAO Qing, ZHU Hong-hui   

  1. 1.Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology, South China (The MinistryProvince Jointly Development), Guangzhou 510070, China;
    2.College of Horticulture, South China Agricultural University, Guangzhou 510642, China
  • Received:2013-05-02 Online:2013-07-25 Published:2013-07-03

新疆阿克苏盐碱地土壤细菌的分离研究

李良秋1,郑贺云1,蔡卓平1,姚青2,朱红惠1*   

  1. 1.广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 省部共建华南应用微生物国家重点实验室, 广州 510070;
    2.华南农业大学园艺学院, 广州 510642
  • 通讯作者: 朱红惠,研究员,主要从事微生物资源环境方向研究。E-mail:zhuhonghui66@yahoo.com.cn
  • 作者简介:李良秋,高级工程师,研究方向为微生物资源与发酵工程。
  • 基金资助:

    国家自然科学基金项目（31170009）；广东省自然科学基金项目（10151007002000008）；新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放基金课题重点项目（BRZD1004）资助。

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Abstract: In order to understand the diversity of microbial resources in culture media from the saline soil of Tarim Basin in Xinjiang, we isolated and cultured 103 strains of the bacteria from the saline alkaline soils of Akesu in Xinjiang. 16S rDNA sequence phylogenetic analysis showed that these strains belong to the genus Bacillus and other 31 genera. The dominant strains were Bacillus, Streptomyces, Micrococcus, Kocuria and Paracoccus，which occupied 36.27%, 10.8%, 6.9%, 6.9% and 3.9% of the total strains. 16S rDNA of five strains had less than 97% sequence similarity to those published strains, suggesting that they might be new bacterial taxa. The results showed that there were abundant micriobial resources in the saline alkaline soils of Aksu in Xinjiang, providing an important base for the development of microbial resources in saline alkaline soils.  

Key words: Akesu, saline alkaline soils, bacteria, diversity analysis, 16S rDNA

摘要： 为从可培养层面了解新疆塔里木盆地盐碱地土壤中的细菌资源多样性，本研究共分离和培养盐碱地土壤中的细菌103株。16S rDNA基因序列系统发育分析表明，这些株菌分别属于芽胞杆菌属（Bacillus）等31个属。盐碱地土壤环境中的优势菌群是Bacillus, Streptomyces, Micrococcus, Kocuria和Paracoccus,分别占总菌株数的36.27%、10.8%、6.9%、6.9%和3.9%。其中5株菌的16S rDNA基因序列与已有效发表菌株的序列相似性小于97%,表明它们可能是新的细菌分类单元。研究表明新疆阿克苏地区盐碱地土壤中具有丰富的微生物资源,为盐碱地微生物资源的开发提供重要的理论依据。

关键词: 阿克苏, 盐碱地, 细菌, 多样性分析, 16S rDNA基因

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