Table of Content

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• Current Biotechnology
  Volume 2 Issue 5 25 September 2012
• Development Status and Genetic Breeding Research Priorities of Barley in China
• BIAN Xiu-xiu1, LI Zhi-lan2, REN Hong-yan3, WANG Dao-jie4, YANG Xin-quan3*
• 2012, 2(5):  309-314.  DOI: 10.3969/j.issn.2095-2341.2012.05.01
• Abstract ( ) PDF (1248KB)( )
Barley is one of important crops in the world,and is an important raw material in the beer and the feedstuff industry. In this paper, the market potential, growth status and breeding techniques of barley were compared with other countries, the necessity and imminency for the more advanced techniques of breeding in barley were illustrated, the important field in genetics and breeding of barley was brought out, and the project for enhancing study on genetic and breeding of barley was explored.
• Strategies Involved in the Phytic Acid Metabolism Studying in Maize
• ZHANG Shao-jun, CHEN Ru-mei
• 2012, 2(5):  315-322.  DOI: 10.3969/j.issn.2095-2341.2012.05.02
• Abstract ( ) PDF (2863KB)( )
Phytic acid is abundant in the seeds of crop especially in maize. Originally, the excellent work of the breeders foucus on the remarkebale reduction of the phytic acid and the increasing of inorganic phosphorus in seeds. While, as indeep research of phytic acid, people have known that this strange compound has irreplaceable function for both plant and animal. Phytic acid is an antinutritional factor, but it is also an important healthy factor for human and animal. For plant, the function of phytic acid is poorly known. Knowledge about its synthesis and its functions is poorly understood yet, since we know its existence early. We must have an accurate knowledge about its synthesis pathway if we want to grasp the right oreintation of the phytic acid breeding. For the past few years, a few researches focusing on the metabolism of phytic acid have been reported. Here, we review the functions of phytic acid and the progress concerning its metabolism research; describe and analyze its pathway in plant, and the four different strategies involved in the studying of phytic acid metabolism. Those strategies would promotes the progerss of the inositol phosphates research in the future.
• Applications of Barcode Microfluidic Technology in Detecting Secreted Proteins
• Qiao Dong-juan, Cai Ji-ye*
• 2012, 2(5):  323-327.  DOI: 10.3969/j.issn.2095-2341.2012.05.03
• Abstract ( ) PDF (1799KB)( )
Microfluidic chips have the features of controllable liquid flow, less sample and reagents consumption, faster analysis speed. It can be used to analyze hundreds of samples in a few minutes or even shorter time, and be sued to realize the sample online pretreatment and analyze the whole process. This paper introduced a barcode microfluidic chip, which with the peculiarity of high density of ssDNA as templates. Traditional protein microfluidic chip had the shortcoming of denaturating easily once fixed on the solid surface. Barcode microfluidic chip not only solved the requirement of stability but also met the requirement of handling large amounts of data. It can be used to detecte lots of cell secretory protein fast. Barcode microfluidic chip is becoming the most attractive analysis tools of detecting secreted proteins, because of its characteristics of simple sample preparation, low consumption and high efficiency and high flux, which has been widely used in the fields of analyzing and detecting sample and clinical research.
• Research Advances in Molecular Biology of Biosynthesis of Microcystins and Their Detection
• YUAN Zhi-yong1,2, ZHU Hong-hui1*, HUO Guang-hua2, FENG Guang-da1
• 2012, 2(5):  328-334.  DOI: 10.3969/j.issn.2095-2341.2012.05.04
• Abstract ( ) PDF (1983KB)( )
Along with the globally increasing eutrophication in waterbody, the frequency and extent of cyanobacterial blooms are overwhelmingly increasing, which has become one of the most serious environmental hazards. In particular, blooms caused by Microcystis frequently occur in some large lakes in China, imposing severe harm to ecosystem and human beings. In this study, chemical structure and damage of Microcystins are summarized; biosynthesis of microcystins is reviewed focusing on the mcy gene cluster, formation of Adda, and peptide chain cyclization; molecular biology techniques including polymerase chain reaction (PCR), real-time quantitative PCR detecting system (QPCR), gene microarray, and denaturing gradient gel electrophoresis (DGGE) are introduced. Finally, further perspective is also put forward.
• Recent Advances in Mangrove Actinomycetes
• WU Shui-long, JIANG Li-ming*
• 2012, 2(5):  335-340.  DOI: 10.3969/j.issn.2095-2341.2012.05.05
• Abstract ( ) PDF (1231KB)( )
Mangroves grown in tropical and subtropical coastal areas are unique inter-tidal zone plant communities with high moisture, high salinity, hypoxia tolerance, and high biodiversity. Mangroves are also precious resources for screening and discovering new actinomycetes species and medicinal bioactive secondary metabolites. At the aspects of species identification, taxonomy of actinomycetes is an essential method in recent mangrove actinomycetes research. This review is mainly focused on the application of taxonomy of actinomycetes, the characteristics of ecological environment and species distribution, and bioactivity of secondary metabolites in soil actinomycetes and endophytic actinomycetes in mangroves. We also prospected the future development trends on these aspects.
• Application Progress of Suppression Subtractive Hybridization in Screening Differentially Expressed Genes of the Poultry
• XU Wen, LUAN Xin-hong*, CAO Zhong-zan, LIU Mei, Gao Ming
• 2012, 2(5):  341-344.  DOI: 10.3969/j.issn.2095-2341.2012.05.06
• Abstract ( ) PDF (1143KB)( )
Suppression subtractive hybridization (SSH) is a kind of technology which is now widely used to find differences in gene expression.  It is accepted by most researchers because of its characteristics of high efficiency, stability and reliability. The advantage of SSH is that it can be used in different environment, different physiological condition of tissues or cells to research the expression of genes. In recent years, with the development of molecular biology, screening and cloning differentially expressed genes has gradually become the research focus. This review summarizes the current research about SSH using in production performance, diseaseresistant mechanism and variety differences of goose, duck and chicken, thus providing more reference for using SSH to research the life activities of the molecular mechanism.
• Electrolytic Stimulating Technique and its Application to Biochemical Engineering
• SONG Bo
• 2012, 2(5):  345-348.  DOI: 10.3969/j.issn.2095-2341.2012.05.07
• Abstract ( ) PDF (1144KB)( )
Electrolytic stimulation technology is to use the electrolysis method that needed to insert the electrode into electrolyte, forming a kind of electrolysis pool system. The electrolyte may make different effects on cell culture, and result in biological processes change. Electrolytic stimulation has two aspects of roles on microbes: promotion and exterminate action, so it has been applied in microbial engineering, such as promoting microbial growth and metabolism, strengthening the wastewater treatment, bioremediation and sterilization, etc. Research shows that the electrolytic stimulation has broad application prospect in microbial engineering and environmental biological engineering fields.
• Identification and Phylogenetic Analysis of a Streptomycete Strain 18-A-5 with Blue Diffusible Pigment
• LI Liang-qiu, YANG Song-zhen, ZHU Hong-hui*
• 2012, 2(5):  349-353.  DOI: 10.3969/j.issn.2095-2341.2012.05.08
• Abstract ( ) PDF (1259KB)( )
A strain 18-A-5 produced blue diffusible pigment was isolated from a tropical rainforest soil sample. Morphological observation showed that on Gause's synthetic agar medium the strain produced large quantities of blue diffusible pigment, blue substrate mycelium, grey-white aerial mycelium, grey spore, long spore chains straight or flexuous, spores were cylindrical and smooth. The genomic DNA G+C content of the strain was 62.4%. Analysis of the 16S rDNA gene sequence(GenBank accession no. EU054353) of strain 18-A-5 revealed that the highest similarity (100%) was to Streptomyces indigoferus ATCC23924^T and Streptomyces herbaricolor ATCC23924^T. Phylogenetic tree of Streptomyces 16S rDNA gene sequences showed that 18-A-5 strain was clustered with Streptomyces indigoferus and Streptomyces herbaricolor, the confidence branch was 74%.On the basis of its physiological and molecular properties, it is evident that the strain 18-A-5 belongs to Streptomyces herbaricolor. The physicochemical characterization of pigment was studied.
• Isolation, Identification and Bioactivity Screening of Marine Actinobacteria
• XIN Yan-juan, AI Jiang-ning, LIU Ya-nan, WU Pei-chun, CAO Xu-peng, XUE Song*
• 2012, 2(5):  354-358.  DOI: 10.3969/j.issn.2095-2341.2012.05.09
• Abstract ( ) PDF (1186KB)( )
An actinobacteria D164 was isolated from marine sponge, which has strong bioactivity. According the 16S rRNA genes sequences analysis, physiological and biochemical characterizations, the stain D164 was identified as Streptomyces rochei. The strain D164 fermentation products not only have high resistance to agricultural pathogenic bacteria, but also have high pesticide and herbicide activity, in which the active compounds deserve further study.
• The Research of Glass Powder Absorbing Nucleic Acid and its Application of Nucleic Acid Extraction from Plant Tissue
• He Hai-wang1,2, Xu Chun-yan1, Wen Li1, He Long-fei1*, Li Chuang-zhen1, Wei Shan-qing1
• 2012, 2(5):  359-365.  DOI: 10.3969/j.issn.2095-2341.2012.05.10
• Abstract ( ) PDF (1461KB)( )
In order to study the application of nucleic acid extraction from plant tissue, the optimum condition of glass powder binding to nucleic acid was gained by comparing the effects of diameter of glass powder, chaotropic salt kinds and concentrations, pH on nucleic acid binding to glass powder. The results showed that glass powder had higher ability to absorb nucleic acid than silica and diatomite, and glass powder with 83 μm diameter was better. The glass powder has the strongest absorption ability under pH 4.0. The NaCl concentration should be higher then 3 mol/L in order to gain the highest yield of DNA, and glass powder can better absorb RNA with more than 2 mol/L guanidine thiocyanate. Besides, it was more effective by adding more than 50% anhydrous ethanol before glass powder absorb RNA. Nucleic acid extracted from plant tissue through the simple purification column, that was made by glass powder, can be applied for restriction digestion, hybridization and PCR. Compared with traditional methods, using glass powder column to entract nucleic acid from plant tissue is effective, envionmentally friendly, fast and economic.
• Philippines Agricultural Biotechnology Annual 2011
• Perfecto G. Corpuz, William Verzami*
• 2012, 2(5):  366-376.  DOI: 10.3969/j.issn.2095-2341.2012.05.11
• Abstract ( ) PDF (989KB)( )
The Philippine biotechnology regulatory system remains sciencebased and the country is increasingly being looked up to for guidance on biotechnology policy and regulations by other developing countries. Commercialization of the first locally developed genetically enhanced crop (Bt eggplant) will likely take place by early 2013, and Golden Rice is likely to follow shortly there after. There have been no reported biotechnology related trade disruptions, and U.S. exports of GE products continued to grow in 2010. Philippine agricultural policy remains food security oriented and policy developments have been positive. However, in the recently approved Philippine Development Plan, there are provisions that support the labeling of foods derived from GE products, as well as a precautionary approach to environmental risk assessments.