Table of Content

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• Current Biotechnology
  Volume 2 Issue 6 25 November 2012
• The Progress on Microspore Culture and Genetic Transformation of Triticeae Crops
• LIU Da-pu, YANG Feng-ping, ZHANG Xiao-dong
• 2012, 2(6):  385-390.  DOI: 10.3969/j.issn.2095-2341.2012.06.01
• Abstract ( ) PDF (1208KB)( )
Microspore, a single haploid cell, can produce a lot of double haploid plants by in vitro culture. The plants will not only provide stable materials for molecular biology, genetics, and morphological genology and so on, but also the novel direction and thought to transgenic research. The basic steps and key factors on microspore culture of Triticeae crops, and the methods of microspore genetic transformation in wheat were introduced in this paper.
• The Biological Characteristics of Sunflower Broomrape and Research Progress on Antibroomrape Molecular Breeding
• PANG Jun-feng, MA De-ning, WANG De-shou, LI Lian-she, WU Yan-min
• 2012, 2(6):  391-396.  DOI: 10.3969/j.issn.2095-2341.2012.06.02
• Abstract ( ) PDF (1123KB)( )
Sunflower is one of the important oil crops in the world, and also a significant economic crops for foods. Sunflower broomrape is a kind of weed seriously harmful to the growth of sunflower, and it happened all over the world. Broomrape seed has quite strong vigor and it is difficult to eradicate completely. The research of sunflower broomrape and anti-broomrape sunflower breeding become important for agricultural scientific researcher. This paper summarized the biological characteristics of sunflower broomrape, feature of parasitic, propagation and harm symptoms, the management measures of prevention and control, analyzed and prospected the research progress of antisunflower broomrape molecular breeding.
• The Advance of Vitamin E Genetic Engineering in Plants
• ZHANG Liang, ZHANG Lan, WANG Lei
• 2012, 2(6):  397-403.  DOI: 10.3969/j.issn.2095-2341.2012.06.03
• Abstract ( ) PDF (1821KB)( )
Vitamin E, a lipid-soluble vitamin, is one of the most important antioxidants in organisms. In this review, we summarize the latest research progress of vitamin E biosynthesis pathway and related genes function, and propose three ways to improve the vitamin E content in the plants by genetic engineering, e.g. to improve the level of metabolic substrates, to increase total tocopherol contents and to raised the α-tocopherol phenol proportion. Furthermore, we analyze the factors to be considered in the process of vitamin E genetic engineering. Finally, the future research directions of vitamin E quality improvement in crops are prospected.
• Mechanism of the Pectinases Used for Bast Fibre Bio-degumming
• ZHENG Ke, LIU Zheng-chu, DUAN Sheng-wen, CHENG Li-feng, ZHENG Xia, FENG Xiang-yuan
• 2012, 2(6):  404-410.  DOI: 10.3969/j.issn.2095-2341.2012.06.044
• Abstract ( ) PDF (1135KB)( )
Degumming is a key problem in bast fibre industry while biological method is the development trend of fibre extraction, and pectinases application in biological degumming is the hotsopt of the research. In this paper, the mechanism of the pectinases used for bast fibre degumming has been expounded according to many studies on the pectinases and the pectinase-producing strains. Pectinase is considered as an essential enzyme for bast fibre bio-degumming, but it can not complete degumming independently. The research in the future will focus on constructing efficient strains, which secrete the whole indispensable enzymes by genetic manipulation methods.
• Advances in Research of Microbial Fermentation Bed in Pig Farming
• LAN Jiang-lin, LIU Bo, SONG Ze-qiong, SHI Huai, HUANG Su-fang
• 2012, 2(6):  411-416.  DOI: 10.3969/j.issn.2095-2341.2012.06.05
• Abstract ( ) PDF (1132KB)( )
Raising pigs on biology bed was a new and environment friendly technology that based on natural agriculture and microbial fermentation. This paper reviewed on micriobial fermentation bed research in China from aspects of technical principles, composition and managing technology, microbial community structure, inhibition on pathogenic microorganisms, volatile components of fermentation bed and utilization of waste litter, analyzed the existing problems in the application of the technology, and finally proposed building the microbial fermentation bed resource utilization system as the future focus of research and development direction.
• Prokaryotic Expression of Trunked CD163 Receptor of PRRSV
• LI Hong, Yi Jian-zhong
• 2012, 2(6):  417-422.  DOI: 10.3969/j.issn.2095-2341.2012.06.06
• Abstract ( ) PDF (1752KB)( )
In order to prokaryotically express the CD163 protein, one receptor of porcine reproductive and respiratory syndrome virus (PRRSV), we predicted the structure of CD163 using ScanProsite software, and fragmented it. CD163 fragments from primary alveolar macrophages (PAM) were amplified by RT-PCR using specific primers based on the known sequence from pig PRRSV(GenBank accession no. EU016226). Three fragments of CD163 were amplified and cloned into the prokaryotic expression vector pET-28a (+), the recombination proteins of CD163 fragments were expressed in E. coli cells. Western blot test verified that the expressed recombinant proteins had good antigen activities.This work provided the experimental basis for further study of the CD163 receptors role in the process of viral infection, and the interaction with other receptors.
• A Primary Study on the Enzymatic Properties of a Keratin-degrading Bacterium
• LIU Xiao-di, GU Zhen-hong, ZHU Hong-hui, YAO Qing,
• 2012, 2(6):  423-427.  DOI: 10.3969/j.issn.2095-2341.2012.06.07
• Abstract ( ) PDF (1298KB)( )
A feather keratindegrading strain (Sporosarcina GIMN1.015) was isolated from soil mixed with feather by enrichment culture. The influences of temperature, initial pH, supplementary carbon sources and feather content on the proteolytic activity were investigated with natural feather as the substrate. The results showed that the proteolytic activity in the feather medium was the highest at 30℃ with initial pH 11.0. Compared with the medium with feathers only, addition of glucose increased proteolytic activity. With the substrate content of 1.5%, the proteolytic activity was highest. The results laid the foundation for further application of feather as resource using keratindegrading microorganisms.
• Simulation of Synthetic Natural Gas (SNG) Production from Biomass Gasification
• FENG Fei, SONG Guo-hui, SHEN Lai-hong, XIAO Jun
• 2012, 2(6):  428-435.  DOI: 10.3969/j.issn.2095-2341.2012.06.08
• Abstract ( ) PDF (2184KB)( )
The technology of synthetic natural gas (SNG) production from biomass gasification was described. With Aspen Plus software, the simulating model of the SNG production via interconnected fluidized beds was established and the process was simulated. The effects of the operating conditions of the gasifier, like the gasification temperature and the steam to biomass ratio (S/B), on the methane yield and carbon conversion of the whole system were studied. The simulating results showed that higher gasification temperature and S/B favored gasification process, which resulted in a higher content of biosyngas and higher ratio of H2 to CO. To achieve higher methane yield and carbon conversion, the suitable gasification temperature is 700～750℃, and S/B is about 0.4.
• The Study of a Detecting Technology for Listeria monocytogenes based on F₀F₁-ATPase Molecular Motor Biosensor
• ZHANG Jie1, ZHANG Xin1, FAN Ai-hong2, ZHANG Hui-yuan1, WANG Qi1, GU De-zhou1, WANG Pei-rong3, HAN Jing1, LU Lin1, CHEN Guang-quan1, YUE Jia-chang3
• 2012, 2(6):  436-440.  DOI: 10.3969/j.issn.2095-2341.2012.06.09
• Abstract ( ) PDF (2016KB)( )
A rapid detecting technology was constructed by using chromatophore on the F₀F₁-ATPase molecular motor biosensor for Listeria monocytogenes in the food. Specific prfA probe were connected with F₀F₁-ATPase’s ε subunit by using avidin-biotin system, the test samples and negative sample combined with biosensors, respectively, to compare their ATP synthesis by measuring the amount of H⁺, Listeria monocytogenes  DNA in the samples could be tested. The results showed that optimum conditions for detection was using chro prfA concentration of  0.052 mg/mL and Listeria monocytogenes DNA concentration of  40 ng/mL. By contrasting to conventional detection methods and PCR detection method to test the actual samples, this method has good conformity.
• Japan Agricultural Biotechnology Annual 2011
• Jeffrey Nawn, Suguru Sato*
• 2012, 2(6):  441-455.  DOI: 10.3969/j.issn.2095-2341.2012.06.10
• Abstract ( ) PDF (1032KB)( )
This report provides the latest status of Japanese consumption, regulation, public perception, research and production of biotech crops.