Abstract: Microfluidic chips have the features of controllable liquid flow, less sample and reagents consumption, faster analysis speed. It can be used to analyze hundreds of samples in a few minutes or even shorter time, and be sued to realize the sample online pretreatment and analyze the whole process. This paper introduced a barcode microfluidic chip, which with the peculiarity of high density of ssDNA as templates. Traditional protein microfluidic chip had the shortcoming of denaturating easily once fixed on the solid surface. Barcode microfluidic chip not only solved the requirement of stability but also met the requirement of handling large amounts of data. It can be used to detecte lots of cell secretory protein fast. Barcode microfluidic chip is becoming the most attractive analysis tools of detecting secreted proteins, because of its characteristics of simple sample preparation, low consumption and high efficiency and high flux, which has been widely used in the fields of analyzing and detecting sample and clinical research.

Key words: microfluidicss, secreted proteins, PDMS

摘要： 微流控芯片具有液体流动可控、消耗试样少、分析速度快等特点，它可以在几分钟甚至更短的时间内进行上百个样品的同时分析，并且可以实现在线样品的预处理及分析全过程。一种条形码微流控芯片能够以高密度的单链DNA为模板，从而克服了传统蛋白质微流控芯片固定在固体表面容易变性的缺点，既解决了稳定性的要求，又满足芯片平行处理大量数据的要求，可以用来大量的、快速的定量检测细胞的分泌蛋白。条形码微流控芯片因其对样品要求简单、低耗高效、高通量等特点正在成为分泌蛋白检测的最具吸引力的分析工具，在样品分析与检测以及临床检测研究等领域得到了广泛的应用。

关键词: 微流控芯片, 分泌蛋白, 聚二甲基硅氧烷