Sreening of Crude Oil Degrading Bacteria and its Degradation Capability

doi:10.3969/j.issn.2095-2341.2014.01.09

Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (1): 44-48.DOI: 10.3969/j.issn.2095-2341.2014.01.09

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Sreening of Crude Oil Degrading Bacteria and its Degradation Capability

WU Pei-chun, WANG Hai-tao, LIU Ya-nan, XIN Yan-juan, CAO Xu-peng, XUE Song

Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning Dalian 116023, China

Received:2013-10-18 Online:2014-01-25 Published:2013-11-26

海绵中石油降解微生物的分离筛选及降解特性研究

吴佩春1,王海涛1,刘亚男1,信艳娟1,曹旭鹏1,薛松1*

中国科学院大连化学物理研究所，海洋生物产品工程组，辽宁大连 116023

通讯作者: 薛松，研究员，主要从事生物活性物质结构与功能研究。Tel：0411-84379069；E-mail：xuesong@dicp.ac.cn
作者简介:吴佩春，实验师，研究方向为海洋微生物。E-mail: peichunwu@dicp.ac.cn
基金资助:
国家自然科学基金项目（31100092）；受格平绿色助学行动辽宁环境科研‘123工程’（CEPF2010-123-2-20）资助。

Abstract

Abstract: A strain of crude oil degrading bacteria OA58 was isolated from a crude oil contaminated marine sponge from Dalian Bay, China. According to the 16S rRNA genes sequences analysis, physiological and biochemical characterizations, the strain OA58 was identified as Streptomyces exfoliatus. When the initial concentration of crude oil was 1 g/L, the strain OA58 could consume 83% of the crude oil in 14 days, indicating a good potential of degradation capability of crude oil.

Key words: crude oil, marine sponge, identification, biodegradation

摘要： 从大连湾原油污染海域生长的海绵中分离筛选到一株原油降解菌OA58，根据其生长形态、培养特征、16S rRNA序列相似性比对分析和生化指标检测，初步鉴定为脱叶链霉菌（Streptomyces exfoliatus）。同时，考察了该链霉菌对原油的降解效果，OA58能以原油为唯一碳源生长，在人工海水培养基中，14 d内对原油（初始浓度为1 g/L）的平均降解率为83%，是一株具有开发潜力的原油降解放线菌。

关键词: 原油, 海绵, 鉴定, 生物降解

WU Pei-chun1, WANG Hai-tao1, LIU Ya-nan1, XIN Yan-juan1, CAO Xu-peng1, XUE Song1*. Sreening of Crude Oil Degrading Bacteria and its Degradation Capability[J]. Curr. Biotech., 2014, 4(1): 44-48.

吴佩春,王海涛,刘亚男,信艳娟,曹旭鹏,薛松. 海绵中石油降解微生物的分离筛选及降解特性研究[J]. 生物技术进展, 2014, 4(1): 44-48.

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Sreening of Crude Oil Degrading Bacteria and its Degradation Capability

海绵中石油降解微生物的分离筛选及降解特性研究

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