Abstract: A pancreatic lipase inhibitor screening model was constructed, which combined with determination of inhibitory activities of amylase, protease and lipase. Three strains FIM-1013, FIL-19 and FIMe-3 were obtained through target screening of microbial broths from soil organisms by this model. The lipase inhibitory activites of FIM-1013,FIL-19 and FIMe-3 were 61%,53% and 42%, the amylase inhibitory activites were 0,7% and 3%, and the inhibitory protease activity were 6%,4% and 0. The results indicated that the three strains could produce very potent selective inhibitor of pancreatic lipase which deserved further research.

Key words: pancrelipase, inhibitor, screening model, protease, amylase

摘要： 本研究采用胰脂肪酶抑制剂筛选模型结合淀粉酶、蛋白酶抑制活性的测定，对来源于土壤的微生物进行高通量筛选，得到三株胰脂肪酶抑制剂产生菌FIM\|1013、FIL\|19和FIMe\|3，其发酵产物对胰脂肪酶活性的抑制率分别为61%、53%和42%，对淀粉酶活性的抑制率分别为0、7%和3%，对蛋白酶活性的抑制率分别为6%、4%和0。结果显示，筛选所得的三个菌株均能产生具有选择性的胰脂肪酶抑制剂，值得进一步的研究。

关键词: 胰脂肪酶, 抑制剂, 筛选模型, 蛋白酶, 淀粉酶