Abstract: In order to establish a rapid chip-based method to identify adulterated mutton, the primers for different animal-sourced components and the reagents required for amplification were freeze-dried and immobilized on blank chip reaction cells beforehand, which could be used in fluorescent quantitative PCR for rapid detection of mutton adulteration. And the performance of prepared chip was evaluated by detection test for simulated adulterated samples, which were made by adding pork, chicken, duck and rat meat into mutton. Compared with ABI7500 fluorescent qPCR, the detection method using rapid chip-based fluorescent qPCR could accurately detect five animal-sourced components, and it had high accuracy and availability. In addition, the time required for PCR amplification of this method was shorter, and it could be easily performed, meeting the requirement for quick detection of mutton adulteration. The preparation of the chip and the establishment of the rapid detection method would effectively simplify the detection process of mutton adulteration and shorten the detection time. Additionally, this method cost lower, and the instruments were easy to carry, which made field testing possible. The research result could provide a strong guarantee for meat safety supervision in China.

Key words: meat product, adulteration, identification, fluorogenic quantitative PCR (real-time PCR), chip

摘要： 为了建立一种基于芯片的快速鉴别羊肉掺假成分的方法，将不同动物源性成分的引物及反应所需试剂预先冻干固定到空白芯片反应池内，以制备羊肉掺假鉴别快速荧光定量PCR芯片。同时，通过模拟掺假样品（在羊肉中掺入猪肉、鸡肉、鸭肉、鼠肉成分）检测实验，对所得芯片的性能进行了评价。从与ABI7500荧光定量PCR结果对比可知，基于芯片的快速荧光定量PCR检测方法可以准确检测5种动物源性成分，具有较高的准确性及可用性，且其PCR扩增时间较短，操作简单，满足了羊肉掺假快速鉴别的要求。该芯片的研制及快速检测方法的建立将有效的简化羊肉制品掺假检测的步骤、缩短检测时间，且成本较低，仪器便于携带，使现场检测成为可能。研究结果为我国肉类食品安全监管提供了有力保障。

关键词: 肉制品, 掺假, 鉴别, 荧光定量PCR, 芯片