Abstract: According to the genome analysis of Trichoderma lentiforme ACCC30425, the coding sequences of 15 lipase genes were annotated, and then used for gene synthesis and heterologous expression in Escherichia coli BL21. 11 lipase-producing clones were obtained, and the highest lipase activity was up to 1.5 U/mL. Results of preliminary enzymatic analysis indicated that the recombinant lipases had the characteristics of heat resistance and alkali resistance universally, showing optimal activities at 50~90℃ and pH 8.5~9.5. Among them, the optimum temperature of LipP was 90℃, which was much higher than all known counterparts, and LipQ showed optimal activity at 50℃, which was close to the intermediate temperature of detergent industry. And other lipases were also thermostable lipase, showing optimal activities at 70℃ or 80℃.

Key words: Trichoderma, lipase, prokaryotic expression, heat resistance

摘要： 基于在Trichoderma lentiforme ACCC30425全基因组序列注释的15个脂肪酶基因，进行脂肪酶基因合成与Escherichia coli BL21异源表达，获得11个产脂肪酶阳性克隆，其中酶活力最高为1.5 U/mL。经初步酶学分析，结果表明重组表达脂肪酶普遍具有耐热性、耐碱性的特征，最适温度范围为50~90℃，最适pH范围为8.5~9.5。其中，LipP最适温度为90℃，远高于目前已知脂肪酶的最适温度；LipQ最适温度为50℃，最接近洗涤工业的中温温度；其他脂肪酶最适温度为70℃或80℃，均属耐热脂肪酶。

关键词: 木霉, 脂肪酶, 原核表达, 耐热性