Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis

doi:10.19586/j.2095-2341.2022.0207

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (2): 220-227.DOI: 10.19586/j.2095-2341.2022.0207

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Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis

Yamei ZHOU(), Jia LIU, Dan LU, Qingxin KONG()

Department of Pharmaceutical Engineering，Chongqing Chemical Industry Vocational College，Chongqing 401228，China

Received:2022-12-08 Accepted:2023-02-17 Online:2023-03-25 Published:2023-04-07
Contact: Qingxin KONG

Abstract

Abstract:

As a commonly used biocatalyst， lipase is widely used in pharmaceutical， food and biochemical applications. However， the application of lipase in enzyme catalysis industry was limited because of its poor stability of free lipase， susceptibility to the environment and poor reusability. In response to the shortcomings of free lipase in the field of catalysis， enzyme immobilization technology was developed. The immobilization of lipases has greatly improved their original catalytic activity and stability and has led to a wider range of industrial applications. The use of immobilized lipases with their own excellent properties selectively catalyses the synthesis of the desired product with mild reaction conditions， high yields and few side reactions. The research and applications of lipase immobilization and its use in pharmaceutical synthesis were reviewed and the future of immobilized lipases was given， which was expected to provide reference for the application of immobilized lipase in industry.

Key words: lipase, immobilization, catalysis, pharmaceutical synthesis

CLC Number:

Q814.2

Yamei ZHOU, Jia LIU, Dan LU, Qingxin KONG. Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis[J]. Current Biotechnology, 2023, 13(2): 220-227.

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https://swjsjz.magtechjournal.com/EN/Y2023/V13/I2/220

Figures/Tables 8

Fig. 1 Enzyme immobilization methods

Fig. 2 Graphene oxide immobilized lipase[12]

Fig. 3 Lipase entrapped in molecular cages on magnetic microsphere surface[13]

Fig. 4 Chitosan immobilized lipase[14]

Fig. 5 The enhanced MOF-immobilized lipase CAL-A with polyethylene glycol[17]

Fig. 6 One-step in situ enzyme immobilization via electropolymerization of conductive polymer[23]

Fig. 7 Microwave assisted Novozym 435 catalyzed kinetic resolution of ketorol[29]

Fig. 8 Kinetic resolution of (±) ethyl-3-hydroxy-3-phenyl propanoate by immobilized enzyme[32]

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Progress on Lipase Immobilization and its Application in Pharmaceutical Synthesis

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