Current Biotechnology

Progress on Plant Resistance Mechanism to Waterlogging Stress
JI Yan-|lin1, LAI Hui-ling2, ZHENG Ru-|ping1, DING Hui1, SUN Wei-|hong1, ZOU Shuang-|quan1*
2016, 6(1): 1-5. DOI: 10.3969/j.issn.2095-2341.2016.01.01
Abstract ( ) PDF (1051KB)( )

In order to adapt to waterlogging stress, stress mechanism would be activated according to the characteristics while respiratory manner changed from aerobic respiration to anaerobic respiration. The paper analyzed plant resistance mechanism to waterlogging stress from the changes in plant morphology, plant physiological metabolism, plant stress response and soil microbes. The paper also put forward prospect for the research on the relationship between response mechanism of microbial ecology and waterlogging stress, which was expected to provide suggestions for further research on mechanism of plant stress.

Progress of Proliferation Methods and Pharmacological Component of Euscaphis Plants
LAI Hui-ling1, JI Yan-lin2, LOU Yang3, WANG Qiao2, LI Peng-fei2, ZOU Shuang-quan2*
2016, 6(1): 6-9. DOI: 10.3969/j.issn.2095-2341.2016.01.02
Abstract ( ) PDF (1058KB)( )

Euscaphis is rare wild plants that rewarded of dual-use for both medicine and appreciation, which can be obtained by seed propagation, tissue culture propagation and cutting propagation. Pharmacological studies on Euscaphis are quite abundant, including anti-inflammatory, inhibition of cell proliferation and control of microorganism and insects. This paper reviewed the research progress of propagation techniques and pharmacological component of Euscaphis plants. At last, the paper outlooked the emphasis and major trends of microbiological studies of Euscaphis according to population ecology, cultivation, pests control and ecological diversity.

Progress on Bacterial Cold Shock Proteins
JIN Xin, FAN Hai-li, CHEN Jian-li, HUANG Tian-pei*, GUAN Xiong
2016, 6(1): 10-13. DOI: 10.3969/j.issn.2095-2341.2016.01.03
Abstract ( ) PDF (1045KB)( )

Cold shock proteins (CSPs) is the 7 kDa small protein that bacteria responsing to low temperature. CSPs is closely related to cell cold response. CSPs may function as RNA chaperones, as they possess binding mRNA. In this way they could minimize the secondary folding of mRNA, thereby facilitating the translation process. This article reviewed the function, application and mechanism of regulation of CSPs, which was expected to provide reference for further study on the application and function of CSPs.

Progress of the Natural Evolution and Action Mechanism of Cry Toxin
ZHENG Qi-lian1,2, GUO Ya-jie2, LIN Qian-nan2, CHEN Xiao-gang3, LI Zong-di3, DAI Xiao-yi3
2016, 6(1): 14-18. DOI: 10.3969/j.issn.2095-2341.2016.01.04
Abstract ( ) PDF (1734KB)( )

Insecticidal Cry toxins produced by Bacillus thuringiensis are used worldwide in transgenic crops for efficient pest control. Among the family of Cry toxins, the three dimensional structure domain of Cry family leading to the natural evolution, and producing a large number of Cry proteins with similar structure and mode of action but different insect specificity. Also, this group is the basis of the molecular study on insect specificity. This article briefly described the Bt and its development. Furthermore, the paper expounded the natural evolution on the insect specificity and its action mechanism of Cry toxins. Finally, we proposed a general strategy for in vitro evolution of Cry toxins insecticidal activity.

Progress on Molecular Modification Methods of Insecticidal Activity of Cry Toxin
GUO Ya-jie1,2, LI Ping1, WU Zhong-ling1, ZHENG Ru-ping1, ZHANG Jun1,2, HU Xia1*
2016, 6(1): 19-24. DOI: 10.3969/j.issn.2095-2341.2016.01.05
Abstract ( ) PDF (1107KB)( )

Cry toxins produced by Bacillus thuringiensis (Bt) could provide a valuable resource for efficient pest control. But insect midgut proteases could be a limiting step of Cry toxicity in different insect species. This article analyzed several different strategies of improving toxicity against a specific target, including domain Ⅲ swapping, domain Ⅱ and domain Ⅲ mutations and other mutations in Cry toxins. Furthermore, this article also expounded the phage display to optimize toxin, which improved the insecticidal toxicity against pests that show no susceptibility to the parental Cry toxins. The paper was expected to provide reference for molecular modification and application of Cry toxin.

Progress on Prevention and Control Methods for Empoasca vitis
LIN Gui-fang, ZHANG Ling-ling*
2016, 6(1): 25-29. DOI: 10.3969/j.issn.2095-2341.2016.01.06
Abstract ( ) PDF (1071KB)( )

Empoasca vitis is one of the most destructive insects in Chinese tea plants. With a growing demand of safe and quality tea, effective approach to prevent the insect has become more important. The prevention and control methods for this insect were discussed in this paper, including chemical control, biological control, physical control and agricultural control. The paper was expected to provide a theoretical basis for comprehensive management of E. vitis.

History of Cellulose and Overview of Nanocrystalline Cellulose
MOU Ya-ni1, ZHANG Jun2, XIONG Yue-ting3, LI Zong-di3, LOU Yang3, LI Ping2, ZHANG Ling-ling1,3*
2016, 6(1): 30-34. DOI: 10.3969/j.issn.2095-2341.2016.01.07
Abstract ( ) PDF (1066KB)( )

With the growing demand of resources such as oil which was scarce and non-renewable, industrial applications using renewable materials has become an inevitable trend. Based on the status, one of the most abundant polymers——nanocrystalline cellulose (NCC) that from natural cellulose has become one of the most promising materials. The article reviewed the progress of the emerging nanomaterial, focused on the history of cellulose and the structure and morphology of nanocrystalline cellulose. The article also introduced the challenges in the production of nanocrystalline cellulose, which was expected to provide reference for research, development and application of NCC.

Extraction and Application of Nanocrystalline Cellulose
WANG Ya-fang1, XIONG Yue-ting2, ZHANG Jun3, LIN Qian-nan3, CHEN Xiao-gang2, XU Gui-xia2, HUANG Bi-wang1*
2016, 6(1): 35-40. DOI: 10.3969/j.issn.2095-2341.2016.01.08
Abstract ( ) PDF (1109KB)( )

The nanocrystalline cellulose which was extracted from cellulose is the most abundant biopolymer and the most promising material in the world. This article reviewed this new nanomaterial, focusing on extraction procedures of nanocomposites derived from woody biomass. The procedures included pretreatment and hydrolysis of lignocellulosic biomass. Besides, the article also discussed the production challenges and the application areas of NCC, which was expected to provide reference for related research.

Characterization and Identification of Pseudomonas aeruginosa Strain BRC-CXG5 and its Toxicity to Monochamus alternatus Hope Larva
MOU Ya-ni1, XU Zhuo-yin2, WENG Xiao-qian2, HUANG Zhi-cheng2, REN Wen-lin2, WU Song-qing3*
2016, 6(1): 41-46. DOI: 10.3969/j.issn.2095-2341.2016.01.09
Abstract ( ) PDF (2263KB)( )

Pseudomonas aeruginosa strain BRC-CXG5, with cytotoxic activity to Monochamus alternatus Hope larva, was isolated from dead larva. The strain was identified by morphological observation, biochemical and physiological characterization and 16S rDNA sequence analysis, results indicated that BRC-CXG5 was affiliated to Pseudomonas sp.. The strain BRC-CXG5 was identified as Pseudomonas aeruginosa, combined with the 16S rDNA sequence analysis. BRC-CXG5 showed high cytotoxic activity to Monochamus alternatus Hope larva based on bioassay data. The results provided a material and theoretical base for the effective development of biological pest control agents.

Isolation and Identification of Bacillus sp. Strain BRC-XYT1 and its Toxicity to Monochamus alternatus Hope
XIONG Yue-ting1, XIA Feng2, HUANG Zhi-cheng2, LIU Li-qin2, WENG Xiao-qian2, WU Song-qing3*
2016, 6(1): 47-51. DOI: 10.3969/j.issn.2095-2341.2016.01.10
Abstract ( ) PDF (1900KB)( )

In this study, strain BRC-XYT1 with cytotoxic activity to Monochamus alternatus Hope larva, was isolated from withered woods. Based on the morphological observation, biochemical and physiological characterization and phylogenetic analysis of its 16S rDNA gene sequence, BRC-XYT1 was identied as one of the strains of Bacillus sp.. When tested with drug sensitive test, strain BRC-XYT1 possessed different levels of sensitivity to a number of common medicine. Besides, strain BRC-XYT1 showed signicant insecticidal activity to Monochamus alternatus Hope larva based on the bioassay data. This study reserved new resource for controlling pine wilt disease and it also provided a theoretical base for the effective development of novel biological pest control agents.

Optimization of Medium for Co-producing Poly-γ-glutamic Acid and 2,3-butanediol by Bacillus licheniformis WX-02
ZHAN Yang-yang1, GAO Li1,2, XU Di-hong3, WEI Xue-tuan4, CHEN Shou-wen1,2*
2016, 6(1): 52-58. DOI: 10.3969/j.issn.2095-2341.2016.01.11
Abstract ( ) PDF (1449KB)( )

Poly-gamma-glutamic acid (γ-PGA) and 2,3-butanediol (2,3-BD) are two important chemicals that can be used in energy, pharmaceuticals and agriculture. The Bacillus licheniformis WX-02 is capable of producing γ-PGA and synthesise 2,3-BD, simultaneously. In this study, the medium for co-production of these two chemicals by B. licheniformis WX-02 was optimized. The pilot scale fermentation was performed in a 50L fermenter. The batch fermentation results showed that the production of γ-PGA and 2,3-BD were achieved as 42.5 g/L and 76.13 g/L, respectively in the optimal medium, which was 26.5% and 188%, respectively increased compared with that before the optimization. The production level for each compound obtained in the co-production system was comparable to that of single compound production system. Collectively, the results laid the foundation for the industrialization of co-production of γ-PGA and 2,3-BD.

Nitrite Dynamics Analysis During Gansu Traditional Jiangshui Fermentation Processing
JIA Ya-li, YUN Jian-min*, AI Dui-yuan, ZHANG Wen-wei, ZHAO Feng-qin, ZHAO Xiao-rui, LI Hong-zhen
2016, 6(1): 59-66. DOI: 10.3969/j.issn.2095-2341.2016.01.12
Abstract ( ) PDF (1525KB)( )

Based on the Gansu Jiangshui traditional fermentation processing, the dynamic changes of physical and chemical indexes were analyzed, including nitrite, nitrate, nitratase, pH, reducing sugar, total acid and the flora of lactic acid bacteria. Meanwhile the degradation effect of lactic acid bacteria on nitrite was explored. The results showed that the nitrite content in the Jiangshui product was 2.76mg/kg after 72h fermentation, which was lower than the requirement in the National Standard (GB2714-2003); the change rule of reducing sugar, pH, total acid and the total number of lactic acid bacteria were illustrated; The results proved that the lactic acid bacteria could reduce the nitrite content in the processing of Jiangshui fermentation.

Isolation and Molecular Identification of Pathogenic Bacteria of Sweet Potato Root Rot Disease
LI Ling-yan1, XIAO Hai-jun1, WANG Wei-qing1, PAN Yan1, KONG Qing-shun2
2016, 6(1): 65-70. DOI: 10.3969/j.issn.2095-2341.2016.01.13
Abstract ( ) PDF (2144KB)( )

The sweet potato root rot disease is a destructive fungus disease in Daxing District of Beijing, in order to determine the dominant fungus species of the disease, we carried out research and fast testing. PCR amplification of ITS and bioinformation analysis were applied in this research. The results were as follows: 2 fungi strains were isolated, molecular identification results showed that both strains were Fusarium oxysporum. This study provided the basis for the rapid identification of sweet potato root rot pathogen, which was expected to provide reference for the prevention of sweet potato root rot.

Study on Control Efficiency of Electrolyzed Functional Water Against Hot Pepper Powdery Mildew
GU Pei-yun1, LU Run-gang1, WANG Bu-yun2, SUN Hai2, YANG Jin-li1, JIAO Xue-xia1, LI Yun-long2*
2016, 6(1): 71-74. DOI: 10.3969/j.issn.2095-2341.2016.01.14
Abstract ( ) PDF (1039KB)( )

In order to explore the control effect of electrolyzed functional water against hot pepper powdery mildew, we sprayed different concentration of electrolyzed functional water in seedling recovering period and preliminary stage of powdery mildew on the hot pepper, and used Bacillus subtilis as control pesticide. The results showed that it could control the disease with better effect when 50 mg/L was used once a week in seedling recovering stage and 80 mg/L was sprayed once a week and lasted for three weeks in the early stage of powdery mildew. When 80 mg/L electrolyzed functional water was only used in the early stage of disease without any treatment in the seedling recovering period, it had certain control efficiency on the disease.

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