Table of Content

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• Current Biotechnology
  Volume 1 Issue 4 25 October 2011
• Food Safety of Bt Cry Proteins from Biotechnology-derived Crops
• WANG Chun-ling, Bruce Hammond*
• 2011, 1(4):  235-241.  DOI: 10.3969/j.issn.2095-2341.2011.04.02
• Abstract ( ) PDF (599KB)( )
Bt microbial insecticides have been used to control larval insect pests that feed on agricultural crops, forest and drinking water for over 50 years. They are also widely used in certified organic agricultural food production in the United States, Europe and other countries. Cry proteins are most commonly introduced in registered Bt crops. Mode of action, food safety and allergenicity of Cry protein have been rigorously tested using rodent, farm animals, and human experiment and bioinformatics research tools. The insecticidal function of Cry protein only works in the alkaline environment of the insect gut where specific receptors on the membranes of midgut epithelial cells present. Other non-target organisms (humans, rhesus monkeys, mice, rats and cows etc.) lack these receptors. Regulatory agencies in US, EU and other countries have confirmed the history of safe consumption of Bt crop and Cry protein residues on crops and in drinking water. Food processing can minimize dietary intake to functionally active Cry proteins. Bt crop contributes to reduce use of insecticides and improves the safe consumption of corn grain by lowering contamination from fumonisin mycotoxins.
• The Research of Reactive Oxygen Species(ROS)in plants
• ZHANG Yi, LU Tie-gang*
• 2011, 1(4):  242-248.  DOI: 10.3969/j.issn.2095-2341.2011.04.03
• Abstract ( )
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• Research Progress of Polyploidy Breeding in Medicinal Plants
• DU Yan-wei, YAN Xiao-guang, ZHAO Jin-feng*
• 2011, 1(4):  249-253.  DOI: 10.3969/j.issn.2095-2341.2011.04.04
• Abstract ( ) PDF (507KB)( )
Polyploid has an important role in medicinal plant breeding, due to the features of the great vegetative organs, strong resistance, high content of medicinal ingredients and so on. This paper introduces the breeding techniques of multiploid, including the principle, methods and identification for artificial induction of multiploid, summarizes the advantages of multiploid of medical plants application and brought forward a few key problems which should be paid attention in polyploid breeding of medical plants and its research prospect.
• Breeding and Molecular Biology Progress of Male Sterile Pepper in China
• LIU Ling1,2, LI Xian-ri2*, WANG Guang-hua2, WANG Dian-chun2, YANG Guan-pin1
• 2011, 1(4):  254-259.  DOI: 10.3969/j.issn.2095-2341.2011.04.05
• Abstract ( ) PDF (558KB)( )
Pepper is often cross-pollinated plants, hybrid seed production cost is higher. The use of male sterility can reduce the cost of hybrid breeding. In recent years, it is becoming a research focus in breeding units and the institutes. This paper introduces the demestic scientific research units and their pepper breeding research, eleborates the progress of the pepper male sterility breeding in China, including genotypes, the CMS source and restorers, describes sterile and maintain gene markers, restoring gene markers developed in recent years and progress of gene cloning, in order to provide a reference to domestic pepper breeding researchers.
• Progress on Agrobacterium tumefaciens-mediated Plant Transformation
• YAO Ran1,2, SHI Mei-li1, PAN Shen-yuan1, SHEN Gui-fang2, ZHANG Zhi-fang2*
• 2011, 1(4):  260-265.  DOI: 10.3969/j.issn.2095-2341.2011.04.06
• Abstract ( ) PDF (610KB)( )
In current, Agrobacterium-mediated gene transferring is one of major methods used in genetic transformation of plants. This paper systematically reviewed the effect on plant transformation efficiency based on the introduction of the principle of this transformation, comparison among different kinds of Agrobacterium strains and the development to transformation vectors. In addition, the newly progresses on the Agrobacterium-mediated transformation of dicotyledonous and monocotyledons species transformation were also discussed, respectively.  
• Biochar Effects on Soil Ecosystem: A Review
• WANG Juan, YAO Huai-ying*
• 2011, 1(4):  266-275.  DOI: 10.3969/j.issn.2095-2341.2011.04.07
• Abstract ( ) PDF (992KB)( )
Biochar is produced by the pyrolysis of natural organic materials under oxygen-limited conditions. The key component element is carbon and the extent of aromatization is very high. The characteristics of biochar include highly porosity, big specific surface area, high charge density and decomposition resistant. Many studies suggest that biochar can change soil CEC and pH, improve soil fertility and health condition, increase crop yield and reduce greenhouse gas emissions. After amendment with biochar, soil microbial biomass is increased and microbial community structure is shift. Nevertheless, previous studies about biochar are still limit on apparent phenomenon. Further studies are needed to understand the inherent micro-mechanism of the biochar effects.
• Eukaryotic Secrete Fusion Expression of Rhizopus oryzae Glucoamylase and Paenibacillus sp. Xylanase
• WANG Zhi1, SONG Li-li2, GU Jin-gang3, DUN Bao-qing1*, TIAN Gu1, LU Ming1, LI Gui-ying1
• 2011, 1(4):  276-281.  DOI: 10.3969/j.issn.2095-2341.2011.04.08
• Abstract ( ) PDF (532KB)( )
The glucoamylase gene (amyA) was amplified from Rhizopus oryzae 3.866 total DNA extracts. The sequence of amyA gene was consisted of 2 049 bp, and encoded 604 amino acids. The xylanase gene (xynA) was amplified from Paenibacillus sp. H10-3 total DNA extracts. The mature peptide encoding sequence of xynA was 636 bp, and encoded 211 amino acids. The mature peptide sequence of amyA gene, sequence of linker and sequence of xynA gene were spliced by overlap extension PCR (SOE-PCR), then amyA-l-xynA was obtained. The fusion gene was then inserted into a Pichia pastoris expression vector pPIC9 to produce the recombinant expression plasmid pPIC9-amyA-l-xynA. By using electroporation, we successfully transformed the recombinant pPIC9-amyA-l-xynA into Pichia pastoris GS115, AX11 was obtained. The maximum yield of the recombinant glucoamylase and xylanase in AX11 culture medium were 5.8 U/mL and 323 U/mL, respectively.
• Growth and Eco-physiological Performances of Soybean under Drought Stress Condition
• WANG Chun-yan1, PANG Yan-mei2, LI Mao-song1*, WANG Xiu-fen1
• 2011, 1(4):  282-288.  DOI: 10.3969/j.issn.2095-2341.2011.04.09
• Abstract ( ) PDF (486KB)( )
The effect of soil water stress on the morphological and physiological characteristics of the seedling stage of the soybean had been studied. The results showed that soybean leaf photosynthesis rate, transpiration rate and stomatal conductance had a downward trend as drought stress increased. The chlorophyll content and leaf water content showed the low-high-low trend with the time course of stress. There were large differences in dry matter accumulation among treatments. At the initial stage of drought stress, the effect of stress on dry matter partitioning ratio was very little, however, as stress increased, the difference increased too. As stress became stronger and advanced, proline content increased significantly, specifically under severe water stress.