Progress on Agrobacterium tumefaciens-mediated Plant Transformation

doi:10.3969/j.issn.2095-2341.2011.04.06

journal1 ›› 2011, Vol. 1 ›› Issue (4): 260-265.DOI: 10.3969/j.issn.2095-2341.2011.04.06

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Progress on Agrobacterium tumefaciens-mediated Plant Transformation

YAO Ran, SHI Mei-li, PAN Shen-yuan, SHEN Gui-fang, ZHANG Zhi-fang

1.School of Life Science, Xuzhou Normal University, Jiangsu Xuzhou 221116, China
2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2011-08-30 Online:2011-10-25 Published:2011-10-11

农杆菌介导的植物遗传转化研究进展

姚冉1,2,石美丽1,潘沈元1,沈桂芳2,张志芳2*

1.徐州师范大学生命科学学院, 江苏徐州 221116
2.中国农业科学院生物技术研究所, 北京 100081

通讯作者: 张志芳,研究员,博士,博士生导师,主要从事基因工程研究。E-mail:zhangzf@mail.caas.net.cn
作者简介:姚冉,硕士研究生,研究方向为遗传学。
基金资助:
姚冉,硕士研究生,研究方向为遗传学。

Abstract

Abstract: In current, Agrobacterium-mediated gene transferring is one of major methods used in genetic transformation of plants. This paper systematically reviewed the effect on plant transformation efficiency based on the introduction of the principle of this transformation, comparison among different kinds of Agrobacterium strains and the development to transformation vectors. In addition, the newly progresses on the Agrobacterium-mediated transformation of dicotyledonous and monocotyledons species transformation were also discussed, respectively.

Key words: Agrobacterium tumefaciens, genetic transformation, progress

摘要： 农杆菌介导的转基因方法是目前植物遗传转化的重要方法之一。本文从农杆菌转化原理、菌株比较及载体发展入手,系统讨论了植物转化受体对转化效率的影响,同时分别综述了农杆菌介导转化技术在双子叶和单子叶植物转化应用中的最新进展。

关键词: 农杆菌, 遗传转化, 进展

YAO Ran1,2, SHI Mei-li1, PAN Shen-yuan1, SHEN Gui-fang2, ZHANG Zhi-fang2*. Progress on Agrobacterium tumefaciens-mediated Plant Transformation[J]. journal1, 2011, 1(4): 260-265.

姚冉,石美丽,潘沈元,沈桂芳,张志芳. 农杆菌介导的植物遗传转化研究进展[J]. 生物技术进展, 2011, 1(4): 260-265.

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Progress on Agrobacterium tumefaciens-mediated Plant Transformation

农杆菌介导的植物遗传转化研究进展

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