Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens

doi:10.19586/j.2095-2341.2024.0122

Current Biotechnology ›› 2024, Vol. 14 ›› Issue (6): 902-910.DOI: 10.19586/j.2095-2341.2024.0122

• Special Forum on Edible and Medicinal Fungi Biotechnology • Previous Articles Next Articles

Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens

Guirong TANG(), Jianyu LIU, Hailong YU, Chunyan SONG, Qi TAN, Xiaodong SHANG()

Key Laboratory of Edible Fungal Resources and Utilization （South），Ministry of Agriculture and Rural Affairs，National Engineering Research Center of Edible Fungi，Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403，China

Received:2024-07-02 Accepted:2024-08-14 Online:2024-11-25 Published:2024-12-27
Contact: Xiaodong SHANG

根癌农杆菌介导的金针菇遗传转化研究进展

唐桂容(), 刘建雨, 于海龙, 宋春艳, 谭琦, 尚晓冬()

上海市农业科学院食用菌研究所，国家食用菌工程技术研究中心，农业农村部应用真菌资源与利用重点实验室，上海 201403

通讯作者: 尚晓冬
作者简介:唐桂容E-mail: grtang@saas.sh.cn；
基金资助:
国家自然科学基金项目(32202572)

Abstract

Abstract:

Flammulina filiformis， a famous medicinal and edible mushroom fungus in china， has a good prospect for development and application. An efficient and stable genetic transformation system is a key technology for studying its gene function. In currently， Agrobacterium-mediated gene transferring is one of the major methods in genetic transformation of F. filiformis. In this paper， key factors involved in Agrobacterium-mediated genetic transformation of F. filiformis incluing recipient tissue， Agrobacterium strains， binary vectors， acestosyringone， and co-culture time were systematically reviewed. The progress of Agrobacteria-mediated transformation technology in the study of gene function of F. filiformis was summarized， and the existing issues and application prospects of this technology were briefly discussed. The aim of this study is to provide a reference for the establishment of an efficient Agrobacterium-mediated genetic transformation system of F. filiformis for the future.

Key words: Flammulina filiformis, Agrobacterium tumefaciens, genetic transformation

摘要：

金针菇（Flammulina filiformis）是一种著名的药食两用真菌，具有良好的开发应用前景。高效稳定的遗传转化体系是其进行基因功能研究的关键技术。农杆菌介导的转基因方法是目前金针菇遗传转化的重要方法之一。系统综述了转化受体、农杆菌菌株、双元载体、乙酰丁香酮和共培养时间等因素对金针菇遗传转化效率的影响，总结了农杆菌介导的转化技术在金针菇基因功能研究中的应用进展，并就该技术存在的问题和应用前景作简单的探讨，以期为今后建立高效的农杆菌介导的金针菇遗传转化系统提供参考。

关键词: 金针菇, 农杆菌, 遗传转化

CLC Number:

Guirong TANG, Jianyu LIU, Hailong YU, Chunyan SONG, Qi TAN, Xiaodong SHANG. Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens[J]. Current Biotechnology, 2024, 14(6): 902-910.

唐桂容, 刘建雨, 于海龙, 宋春艳, 谭琦, 尚晓冬. 根癌农杆菌介导的金针菇遗传转化研究进展[J]. 生物技术进展, 2024, 14(6): 902-910.

Figures/Tables 2

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基因名	功能	受体材料	根癌农杆菌	质粒	启动子	选择标记	参考文献
FIP-fve	调控菌丝生长	菌丝体	LBA4404	pCAMBIA1300	F. velutipes gpd	Hyg	[25]
Fv-ada	调控菌丝生长	菌块	LBA4404	pFungiway	F. velutipes gpd	Hyg	[26]
FfCEL6B	调控菌丝生长	菌块	GV3101	pCAMBIA1300	GpdII	Hyg	[27]
FfGa1	调控菌丝生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[28]
FfGS6	调控菌丝对外界胁迫与子实体生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[29]
Pdd1	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[30]
FFJMHY	调控菌丝、菌柄生长	菌块	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[31]
Fvclp1	调节菌丝生长和子实体发育	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[32]
FvHd1	调控锁状联合形成	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[33]
FvCPC2	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[34]
Fvhom1	调控菌丝生长	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[35]
Hmg1	调节子实体生长速度	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[36]
LFC1	调节子实体生长	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[37]
FFMnSOD1	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FFMnSOD2	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FfNoxA	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]

基因名	功能	受体材料	根癌农杆菌	质粒	启动子	选择标记	参考文献
FIP-fve	调控菌丝生长	菌丝体	LBA4404	pCAMBIA1300	F. velutipes gpd	Hyg	[25]
Fv-ada	调控菌丝生长	菌块	LBA4404	pFungiway	F. velutipes gpd	Hyg	[26]
FfCEL6B	调控菌丝生长	菌块	GV3101	pCAMBIA1300	GpdII	Hyg	[27]
FfGa1	调控菌丝生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[28]
FfGS6	调控菌丝对外界胁迫与子实体生长	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[29]
Pdd1	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[30]
FFJMHY	调控菌丝、菌柄生长	菌块	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[31]
Fvclp1	调节菌丝生长和子实体发育	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[32]
FvHd1	调控锁状联合形成	菌块	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[33]
FvCPC2	调节菌丝生长和子实体发育	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[34]
Fvhom1	调控菌丝生长	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[35]
Hmg1	调节子实体生长速度	菌丝球	GV3101	pBHg-BCA1	A. bisporus gpd	Hyg	[36]
LFC1	调节子实体生长	菌丝球	AGL-1	pBHg-BCA1	A. bisporus gpd	Hyg	[37]
FFMnSOD1	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FFMnSOD2	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]
FfNoxA	调控菌柄长度	菌丝球	GV3101	pBHg-eGFP	A. bisporus gpd	Hyg	[38]

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Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens

根癌农杆菌介导的金针菇遗传转化研究进展

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