Research Progress on the Protective Effects of Hydrogen Gas on Traumatic Brain Injury

doi:10.19586/j.2095-2341.2022.0202

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (2): 234-239.DOI: 10.19586/j.2095-2341.2022.0202

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Research Progress on the Protective Effects of Hydrogen Gas on Traumatic Brain Injury

Jingyi ZHANG(), Xue JIANG, Siyu MA, Zhichao FENG, Yang YI, Chen MA, Yifei SONG, Fei XIE()

Faculty of Environment and Life，Beijing University of Technology，Beijing 100124，China

Received:2022-11-27 Accepted:2023-02-07 Online:2023-03-25 Published:2023-04-07
Contact: Fei XIE

Abstract

Abstract:

Traumatic brain injury （TBI） is a very common disease in daily trauma， and there is still a lack of drugs with definite clinical interventions， so it is important to develop new treatments for TBI. Hydrogen has been proved to have antioxidant， anti-inflammatory， anti-apoptosis and other effects， and has shown a good protective effect in a variety of diseases. In the past decade， many studies have preliminarily confirmed the protective effect of hydrogen on TBI， and the mechanism underlying the effect of hydrogen has also been investigated. The review focused on the protective effect of different hydrogen intervention methods on TBI and the mechanism underlying the effect of hydrogen， and also put forward the deficiencies in the current research and the direction of future research. The paper was hoped to provide reference and help for clinical and basic researchers of hydrogen.

Key words: hydrogen gas, traumatic brain injury, anti-oxidation, anti-inflammation, anti-apoptosis

CLC Number:

R651.15

Jingyi ZHANG, Xue JIANG, Siyu MA, Zhichao FENG, Yang YI, Chen MA, Yifei SONG, Fei XIE. Research Progress on the Protective Effects of Hydrogen Gas on Traumatic Brain Injury[J]. Current Biotechnology, 2023, 13(2): 234-239.

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URL: https://swjsjz.magtechjournal.com/EN/10.19586/j.2095-2341.2022.0202

https://swjsjz.magtechjournal.com/EN/Y2023/V13/I2/234

Figures/Tables 1

References 32

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模型	干预方式	保护效应	可能的作用机理	参考文献
大鼠控制性皮层冲击损伤模型	建模后5 min到5 h吸氢（2%）	减轻血脑屏障通透性渗漏和脑水肿，缓解神经功能缺损	抗氧化	[11]
大鼠控制性皮层冲击损伤模型	建模后30 min到5 h吸氢（2%）	缓解神经功能缺损，减轻血脑屏障通透性和脑水肿	提高miR-21表达水平，抗氧化	[12]
大鼠液压冲击损伤模型	建模后连续7 d每天吸入1 h高浓度氢气（氢气42%，氧气21%，氮气37%）	缓解神经功能缺损	抑制损伤区周围半暗带凋亡反应，抗氧化	[13]
大鼠控制性皮层冲击损伤模型	建模后连续7 d每天吸氢（氢气4%或67%，氧气21%）两次，每次1 h	缓解神经功能缺损	抑制小胶质细胞活化和神经炎症反应	[14]
糖尿病大鼠液压冲击损伤模型	建模后连续2 d或7 d每天吸氢（氢气3%或42%，氧气21%）1 h	呼吸42%氢气可减轻糖尿病大鼠由TBI引起的脑水肿和血脑屏障渗漏，改善神经功能，提高生存率，而呼吸3%氢气无显著效应	抗氧化，抑制神经元凋亡	[15]
大鼠控制性皮层冲击损伤模型	建模后24 h内吸氢（氢气4%，氧气21%）两次，每次1 h	缓解神经功能缺损，改善心脏和肝脏功能	抑制TBI急性期炎症反应	[16]
大鼠液压冲击损伤模型	建模后连续3 d每天吸氢（氢气42%，氧气21%）1 h	提高生存率，减轻脑水肿和肺损伤	抑制细胞焦亡和细胞凋亡，减轻炎症反应	[17]
小鼠控制性皮层冲击损伤模型	从建模前24 h到建模后7 d自由饮用富氢水（1.6 mmol·L^-1）	减轻脑水肿	逆转损伤引起的AQP-4、HIF-1、MMPs和Cyclophilin A表达变化，阻断损伤引起的TAU蛋白磷酸化，调节神经炎症反应，促进细胞呼吸增加ATP产量	[18]
大鼠改良Feeney自由落体撞击模型	建模后连续5 d每天腹腔注射富氢水（5 mL·kg^-1）	神经功能损伤显著减轻，脑水肿、脑出血、细胞坏死明显减少	抗氧化，上调核因子E2 相关因子2（nuclear factor erythroid-2-related factor 2, Nrf2）蛋白水平	[19]
大鼠控制性皮层冲击损伤模型	建模后每天注射一次富氢水（0.63~0.82 mmol·L^-1, 6 mL·kg^-1）	显著抑制TBI引起的生存率下降、脑水肿、血脑屏障渗漏、神经功能缺损	抑制神经炎症反应	[20]
大鼠改良Feeney自由落体撞击模型	建模后5 min腹腔注射富氢水（5 mL·kg^-1）	提高生存率，缓解神经功能缺损	抗氧化，激活Nrf2信号通路	[21]
大鼠液压冲击损伤模型	建模后连续7 d每天腹腔注射富氢生理盐水（0.6 mmol·L^-1，5 ml·kg^-1）	缓解认知功能损伤	抗氧化和调节脑源性神经营养因子（brain derived neurotrophic factor，BDNF）相关的神经元突触可塑性	[22]
小鼠改良Feeney自由落体撞击模型	建模后连续3 d每天腹腔注射富氢生理盐水（>6 mmol·L^-1，5 mL·kg^-1）	缓解神经功能缺损，减轻脑水肿	抑制神经元坏死和神经炎症反应	[23]
神经元划痕损伤模型	富氢DMEM培养液（6 mmol·L^-1）培养48 h	促进神经元突起再生，抑制细胞凋亡，改善细胞活力	激活miR-21/PI3K/Akt/GSK-3β信号通路	[24]
脑微血管内皮细胞划痕损伤模型	富氢DMEM培养液（0.60±0.03 mmol·L^-1）培养	改善细胞活力，抑制细胞自噬	激活PI3K/Akt/GSK-3β 信号通路	[25]

模型	干预方式	保护效应	可能的作用机理	参考文献
大鼠控制性皮层冲击损伤模型	建模后5 min到5 h吸氢（2%）	减轻血脑屏障通透性渗漏和脑水肿，缓解神经功能缺损	抗氧化	[11]
大鼠控制性皮层冲击损伤模型	建模后30 min到5 h吸氢（2%）	缓解神经功能缺损，减轻血脑屏障通透性和脑水肿	提高miR-21表达水平，抗氧化	[12]
大鼠液压冲击损伤模型	建模后连续7 d每天吸入1 h高浓度氢气（氢气42%，氧气21%，氮气37%）	缓解神经功能缺损	抑制损伤区周围半暗带凋亡反应，抗氧化	[13]
大鼠控制性皮层冲击损伤模型	建模后连续7 d每天吸氢（氢气4%或67%，氧气21%）两次，每次1 h	缓解神经功能缺损	抑制小胶质细胞活化和神经炎症反应	[14]
糖尿病大鼠液压冲击损伤模型	建模后连续2 d或7 d每天吸氢（氢气3%或42%，氧气21%）1 h	呼吸42%氢气可减轻糖尿病大鼠由TBI引起的脑水肿和血脑屏障渗漏，改善神经功能，提高生存率，而呼吸3%氢气无显著效应	抗氧化，抑制神经元凋亡	[15]
大鼠控制性皮层冲击损伤模型	建模后24 h内吸氢（氢气4%，氧气21%）两次，每次1 h	缓解神经功能缺损，改善心脏和肝脏功能	抑制TBI急性期炎症反应	[16]
大鼠液压冲击损伤模型	建模后连续3 d每天吸氢（氢气42%，氧气21%）1 h	提高生存率，减轻脑水肿和肺损伤	抑制细胞焦亡和细胞凋亡，减轻炎症反应	[17]
小鼠控制性皮层冲击损伤模型	从建模前24 h到建模后7 d自由饮用富氢水（1.6 mmol·L^-1）	减轻脑水肿	逆转损伤引起的AQP-4、HIF-1、MMPs和Cyclophilin A表达变化，阻断损伤引起的TAU蛋白磷酸化，调节神经炎症反应，促进细胞呼吸增加ATP产量	[18]
大鼠改良Feeney自由落体撞击模型	建模后连续5 d每天腹腔注射富氢水（5 mL·kg^-1）	神经功能损伤显著减轻，脑水肿、脑出血、细胞坏死明显减少	抗氧化，上调核因子E2 相关因子2（nuclear factor erythroid-2-related factor 2, Nrf2）蛋白水平	[19]
大鼠控制性皮层冲击损伤模型	建模后每天注射一次富氢水（0.63~0.82 mmol·L^-1, 6 mL·kg^-1）	显著抑制TBI引起的生存率下降、脑水肿、血脑屏障渗漏、神经功能缺损	抑制神经炎症反应	[20]
大鼠改良Feeney自由落体撞击模型	建模后5 min腹腔注射富氢水（5 mL·kg^-1）	提高生存率，缓解神经功能缺损	抗氧化，激活Nrf2信号通路	[21]
大鼠液压冲击损伤模型	建模后连续7 d每天腹腔注射富氢生理盐水（0.6 mmol·L^-1，5 ml·kg^-1）	缓解认知功能损伤	抗氧化和调节脑源性神经营养因子（brain derived neurotrophic factor，BDNF）相关的神经元突触可塑性	[22]
小鼠改良Feeney自由落体撞击模型	建模后连续3 d每天腹腔注射富氢生理盐水（>6 mmol·L^-1，5 mL·kg^-1）	缓解神经功能缺损，减轻脑水肿	抑制神经元坏死和神经炎症反应	[23]
神经元划痕损伤模型	富氢DMEM培养液（6 mmol·L^-1）培养48 h	促进神经元突起再生，抑制细胞凋亡，改善细胞活力	激活miR-21/PI3K/Akt/GSK-3β信号通路	[24]
脑微血管内皮细胞划痕损伤模型	富氢DMEM培养液（0.60±0.03 mmol·L^-1）培养	改善细胞活力，抑制细胞自噬	激活PI3K/Akt/GSK-3β 信号通路	[25]

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Research Progress on the Protective Effects of Hydrogen Gas on Traumatic Brain Injury

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