Table of Content

• 
• Current Biotechnology
  Volume 1 Issue 1 25 July 2011
• Application of Molecular Marker Technology in Seed Purity Identification of Maize
• LUO Li-ming, LIU Li, YU Li-juan, FAN Xing-ming*
• 2011, 1(1):  7-13.  DOI: 10.3969/j.issn.2095-2341.2011.01.01
• Abstract ( ) PDF (643KB)( )
The paper pointes out the importance of the purity identification of hybrid maize seed based on reviewing the conventional, biochemical and molecular marker identification technologies. Comparing the advantages and disadvantages among the four molecular marker technologies RAPD, RFLP, AFLP and SSR in the identification of purity and validity for hybrid maize seed, SSR is considered as the best choice for hybrid maize seed identification.
• Progress on Expression Vector for Plant Genetic Transformation
• LIU Ya, LI Jing-na, REN Wen, LI Xiang
• 2011, 1(1):  14-20.  DOI: 10.3969/j.issn.2095-2341.2011.01.02
• Abstract ( ) PDF (702KB)( )
The plant expression vector construction for genetic transformation is a very important step in the plant transgenic research, the high efficient expression of exogenous gene in the transgenic plants will be regarded as a very key factor. This paper reviewed research progress on the genetic transformation of plant expression vector in recent years, highlighted in transgenic plants highly expressed exogenous genes to achieve many kinds of ways and the strategy, aimed at enhancing the exogenous gene the expression level and the biological security in the transgenic plants. This paper finally prospects the further plant transgenic research and the commercialization development.
• The Application of Linker Peptide in Multiple Genes Transformation
• SUN He, LANG Zhi-hong*, ZHU Li, HUANG Da-fang
• 2011, 1(1):  21-25.  DOI: 10.3969/j.issn.2095-2341.2011.01.03
• Abstract ( ) PDF (464KB)( )
Multiple genes transformation is one of the hot spots in modern genetic transformation research. Using 2A from foot and mouth virus and LP4 from Raphanus sativus seeds as linkers for multiple genes transformation is a new strategy which can be substitute traditional methods in plant genetic engineering. The linker peptide can be used for connecting multiple genes and genes are expressed coordinately. The hybrid peptide LP4/2A has the both advantages of LP4 and 2A which is a more effective peptide for multiple genes transformation.
• Ku Gene in Microorganisms
• ZHANG Xiao-juan, WEN Yin*
• 2011, 1(1):  26-31.  DOI: 10.3969/j.issn.2095-2341.2011.01.04
• Abstract ( ) PDF (619KB)( )
Non-homologous end joining (NHEJ) mediated by ku genes is a repair type of DNA double-strand breaks (DSBs). NHEJ pathway is independent of homologous recombination (HR), and decreases HR with competitive relationship. ku genes are highly conservative in the biological evolution process, and ku mutants have been phenotypically characterized in many microorganisms, especially in fungi. Gene targeting frequency is increased by deletion of ku genes, when HR plays a key role on the defects of NHEJ pathway, leading to facilitate analysis of gene function.
• Research Progress in Competitive Exclusion Products
• YIN Ye-shi1, ZHANG Yun-cai2, WANG Xin1*
• 2011, 1(1):  32-37.  DOI: 10.3969/j.issn.2095-2341.2011.01.05
• Abstract ( ) PDF (588KB)( )
The concept of competitive exclusion (CE) was first proposed by Nurmi and Rantala in 1973. They described that introduction of gut contents originating from that adult birds can protect young birds against Salmonella infection. Then CE products were introduced in preventing and controling other pathogenic bacteria in gut. CE products have other functions, such as promoting growth, improving feed conversion ratio and decreasing mortality rates. Now, more than 20 kinds of CE products were developed and used in livestock and poultry raise in Europe and America. Although, the mechanism of this product is not clear now, some hypothesis have been proposed, such as competition of the attach site or nutrition, stimulation of the immunity response and generation of  kinds of anti-bacteria products. Because the popular of antibiotic resistance is becoming more serious, CE products will get a very wide application foreground.
• Advance in Methods of Small Molecular Drug Target Discovery
• ZHOU Ting-ting, ZENG Hong-mei*, QIU De-wen
• 2011, 1(1):  38-44.  DOI: 10.3969/j.issn.2095-2341.2011.01.06
• Abstract ( ) PDF (640KB)( )
Many secondary metabolites produced by microbes are small molecular active compounds playing important roles in the medical and agricultural fields. A number of new research methods for small molecular drug targets have come into being driven by genomics, proteomics and bioinformatics, and these methods are based on drug-induced changes in gene or protein expression and various properties of proteins, such as compatibility and stability etc. The new methods promote the discovery of the mechanism of small molecular drugs and exploring new drug targets as resources to further screening for drugs.
• Current Situation of Prevalence, Diagonosis and Prevention of Rabies in China
• JIANG Hui1, CONG Xian-li2
• 2011, 1(1):  45-49.  DOI: 10.3969/j.issn.2095-2341.2011.01.07
• Abstract ( ) PDF (453KB)( )
Of a serious zoonosis, rabies can cause human infection with mortality of 100% almost, and there is no effective method for case treatment so far. This paper introduces the biology character of rabies, elaborates current prevalence state in China, newly developed diagnosis techniques and prevention measures. It would be helpful to control and eliminate rabies in China.
• Cloning of PPDK Gene from Grain Amaranth and its Expression Pattern Analysis
• WANG Miao, WANG Xu-jing, Tang Qiao-ling, WANG Zhi-xing*
• 2011, 1(1):  50-55.  DOI: 10.3969/j.issn.2095-2341.2011.01.08
• Abstract ( ) PDF (721KB)( )
In order to find new gene resources for increasing plant photosynthetic efficiency, pyruvate orthophosphate dikinase gene (PPDK) is cloned from Amaranthus hypochondriacus L. by homology-based cloning and RACE technique. The full length of the cloned gene is 3 224 bp, including 71 bp 5′UTR, 2 868 bp ORF and 285 bp 3′UTR. It potentially encodes a protein of 956 amino acids and its molecular weight is about 106 kDa. Sequence analysis indicated that the deduced protein contained the functional domain of known PPDK gene. The expression pattern analysis of the cloned gene showed that the gene was expressed predominantly in leaf. Based on the results above, it was identified as PPDK gene of Amaranthus hypochondriacus L. and named AhPPDK.
• Prokaryotic Expression, Purification and Crystallization of HIV-1 CRF07 B/C Subtype gp41 Helix-bundle
• WANG Jian-xin1, WANG Min1, ZHANG Tong-cun1, LIU Xin-qi2*
• 2011, 1(1):  56-60.  DOI: 10.3969/j.issn.2095-2341.2011.01.09
• Abstract ( ) PDF (525KB)( )
HIV-1 transmembrane protein gp41, which plays an important role in the fusion of viral and cellular membranes, is the ideal target for intervention of HIV-1 infection. In order to obtain gp41 crystal structure from prevalent strain in China, gp41 helix-bundle region was cloned into pET30-his vector by overlap PCR, enzyme digestion and ligation using CRF07 B/C gp160 gene as template. The plasmid was transferred into E.coli Rosetta(DE3), and expression, purification and crystal screening of gp41 were conducted. Crystals of gp41 helix-bundle were obtained and its structure was solved by molecular replacement method. The overall structure was similar to B subtype, but the unique characteristics in local conformation would facilitate the vaccine design and drug development against CRF07 B/C prevalent in China.
• Cloning, Expression and Characterization of an Alkaline Xylanase from Alkali-tolerant Fungus Pseudallescheria sp. JSM-2
• WANG Kun, LUO Hui-ying, SHI Peng-jun, WANG Ya-ru, YANG Pei-long, YAO Bin*
• 2011, 1(1):  61-67.  DOI: 10.3969/j.issn.2095-2341.2011.01.10
• Abstract ( ) PDF (616KB)( )
An alkali-tolerant fungal strain, Pseudallescheria sp. JSM-2, was isolated from the wastewater of a paper mill. By using degenerate PCR and TAIL-PCR techniques, a full-length xylanase gene, xyl11-1, was cloned from the genomic DNA of strain JSM-2. The complete genomic and chromosomal DNA sequences of xyl11-1 consist of 797 bp and 678 bp, respectively. Deduced XYL11-1 contains a putative 18-residue signal peptide at N-terminus and a C-terminal 207-residue polypeptide. Recombinant XYL11-1 produced in Pichia pastoris GS115 was purified and characterized. The optimal pH of XYL11-1 was 6.5, and the enzyme exhibited more than 50% of the maximal activity at pH 4.5~9.0. XYL11-1 was stable at pH 4.5~12.0. The optimal temperature was found to be 50 ℃. XYL11-1 had a specific activity of 2 618 U/mg towards oat spelt xylan, and was strongly resistant to various neutral and alkaline proteases. In addition to hydrolysis of different xylans, XYL11-1 had broad substrate specificity, including various xylans, cellulose and glucan. Moreover, fermentation of XYL11-1 was easily handled. These superior properties of XYL11-1 make it advantageous for applying in the animal and fish feed and kraft pulp industries.
• Anther Culture of Chinese Cabbage Resistant to Clubroot
• ZHANG Qing-xia, SI Chao-guang, WANG Yuan, ZHANG Shu-xia, LV Xiang-hua, YANG Xiao-yun*
• 2011, 1(1):  68-71.  DOI: 10.3969/j.issn.2095-2341.2011.01.11
• Abstract ( ) PDF (494KB)( )
Chinese cabbage is one of the important vegetables in northern China. Clubroot disease seriously influenced the production of Chinese cabbage in the world. The use of anther culture can greatly accelerate breeding process of Chinese cabbage  resistant to clubroot. Thirty three Chinese cabbage varieties (lines) resistant to clubroot were used to anther culture in this study. The results indicated that the embryo induction rate of varieties were 72.7%, and the highest rate was 1.86 embryos / bud for Oriental Crown×C11 lines. The medium plus 0.4 g/L glutamine was best for induction of most genotypes. The results also showed that donor plants growing in the greenhouse were easier to induce embryoids. Green cotyledon-type embryos were transferred to solid medium with B5+0.2 mg/L BA+0.1 mg/L NAA+0.1% activated carbon to induce adventitious buds.