Screening of Salt-tolerant, Low-Cr-accumulating Hulless Barley Varieties and Their Growth Response Characteristics

doi:10.19586/j.2095-2341.2026.0073

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (4): 926-933.DOI: 10.19586/j.2095-2341.2026.0073

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Screening of Salt-tolerant, Low-Cr-accumulating Hulless Barley Varieties and Their Growth Response Characteristics

Xinping ZHANG¹^,²(), Ma MI¹(), Chongshuang PAN¹, Lu LI¹, Qinghai LIU¹(), Cheng QIU¹, Bin WU¹^,²()

^1.Key Laboratory of Agricultural Product Quality and Safety of Xizang Autonomous Region，Institute of Quality Standards and Testing Technology，Xizang Academy of Agricultural and Animal Husbandry Sciences，Lhasa 850032，China
^2.College of Ecology and Environment，Chengdu University of Technology，Chengdu 610059，China

Received:2026-03-23 Accepted:2026-04-27 Online:2026-07-25 Published:2026-09-11
Contact: Qinghai LIU,Bin WU

Abstract

Abstract:

To address the need for safe hulless barley production and varietal selection in Xizang under the combined pressures of escalating soil salinization and chromium （Cr） environmental risk， this study evaluated eight barley varieties. Under NaCl stress conditions， germination rate， germination index， vigor index and early seedling growth parameters were measured. Based on these results， five varieties were selected for subsequent screening under Cr stress， where root and shoot biomass， Cr content， bioconcentration factor （BCF）， and translocation factor （TF） were determined. The results indicated that NaCl stress significantly inhibited barley germination and seedling growth， with the inhibitory effect intensifying as salt concentration increased. The varietal differences were most pronounced under 200 mmol·L^-1 NaCl. Comprehensive analysis identified Zangqing 148， Zangqing 320， Zangqing 2000， Shanqing 9， and Xila 19 as salt-tolerant varieties suitable for further Cr stress testing. Under Cr stress， the biomass of the five varieties generally decreased with increasing concentration. Cr was significantly enriched in the roots， with all TFs being less than 1. Among the varieties， Shanqing 9 exhibited the lowest Cr content in shoots， as well as the lowest BCF and TF， establishing it as a superior salt-tolerant and low-Cr-accumulating variety. This study provides data support for selecting barley varieties suited to environments with combined salinization and chromium contamination risks.

Key words: hulless barley, salt stress, chromium stress, salt tolerance, low accumulation

CLC Number:

Q945

Xinping ZHANG, Ma MI, Chongshuang PAN, Lu LI, Qinghai LIU, Cheng QIU, Bin WU. Screening of Salt-tolerant, Low-Cr-accumulating Hulless Barley Varieties and Their Growth Response Characteristics[J]. Current Biotechnology, 2026, 16(4): 926-933.

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Figures/Tables 6

References 21

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青稞品种	0 mmol·L^-1	50 mmol·L^-1	100 mmol·L^-1	200 mmol·L^-1
藏青16	96.67±1.15 a	76.00±5.29 b	60.67±4.16 c	22.67±6.11 d
藏青17	95.33±2.31 a	72.67±3.06 b	54.67±5.77 c	26.67±4.71 d
藏青148	97.33±3.06 a	92.00±4.00 b	79.33±6.11 c	35.33±4.16 d
藏青320	98.67±2.31 a	95.33±3.06 b	87.33±4.62 c	45.33±3.06 d
藏青2000	96.00±3.46 a	89.33±5.03 b	74.67±6.43 c	40.00±2.00 d
藏青3000	93.33±1.15 a	66.00±5.29 b	47.33±5.03 c	19.33±6.43 d
山青9号	98.00±2.00 a	84.00±5.29 b	71.33±4.16 c	44.00±2.00 d
喜拉19	97.33±1.15 a	80.00±6.00 b	70.00±4.00 c	39.33±4.16 d

青稞品种	0 mmol·L^-1	50 mmol·L^-1	100 mmol·L^-1	200 mmol·L^-1
藏青16	96.67±1.15 a	76.00±5.29 b	60.67±4.16 c	22.67±6.11 d
藏青17	95.33±2.31 a	72.67±3.06 b	54.67±5.77 c	26.67±4.71 d
藏青148	97.33±3.06 a	92.00±4.00 b	79.33±6.11 c	35.33±4.16 d
藏青320	98.67±2.31 a	95.33±3.06 b	87.33±4.62 c	45.33±3.06 d
藏青2000	96.00±3.46 a	89.33±5.03 b	74.67±6.43 c	40.00±2.00 d
藏青3000	93.33±1.15 a	66.00±5.29 b	47.33±5.03 c	19.33±6.43 d
山青9号	98.00±2.00 a	84.00±5.29 b	71.33±4.16 c	44.00±2.00 d
喜拉19	97.33±1.15 a	80.00±6.00 b	70.00±4.00 c	39.33±4.16 d

青稞品种	0 mmol·L^-1	50 mmol·L^-1	100 mmol·L^-1	200 mmol·L^-1
藏青16	27.55±0.34 a	19.38±1.35 b	13.65±0.94 c	4.42±1.09 d
藏青17	27.17±0.48 a	18.53±0.78 b	12.30±1.30 c	5.20±0.81 d
藏青148	27.74±0.87 a	23.46±1.02 b	17.85±1.37 c	6.89±0.87 d
藏青320	28.12±0.63 a	24.31±0.72 b	19.65±1.04 c	8.84±0.60 d
藏青2000	27.36±0.91 a	22.78±1.23 b	16.80±1.45 c	7.80±0.39 d
藏青3000	26.60±0.36 a	16.83±1.35 b	10.65±1.13 c	3.77±1.25 d
山青9号	27.93±0.57 a	21.42±1.65 b	16.05±0.94 c	8.58±0.39 d
喜拉19	27.74±0.63 a	20.40±1.53 b	15.75±0.90 c	7.67±0.83 d

青稞品种	0 mmol·L^-1	50 mmol·L^-1	100 mmol·L^-1	200 mmol·L^-1
藏青16	27.55±0.34 a	19.38±1.35 b	13.65±0.94 c	4.42±1.09 d
藏青17	27.17±0.48 a	18.53±0.78 b	12.30±1.30 c	5.20±0.81 d
藏青148	27.74±0.87 a	23.46±1.02 b	17.85±1.37 c	6.89±0.87 d
藏青320	28.12±0.63 a	24.31±0.72 b	19.65±1.04 c	8.84±0.60 d
藏青2000	27.36±0.91 a	22.78±1.23 b	16.80±1.45 c	7.80±0.39 d
藏青3000	26.60±0.36 a	16.83±1.35 b	10.65±1.13 c	3.77±1.25 d
山青9号	27.93±0.57 a	21.42±1.65 b	16.05±0.94 c	8.58±0.39 d
喜拉19	27.74±0.63 a	20.40±1.53 b	15.75±0.90 c	7.67±0.83 d

青稞品种	0 mmol·L^-1	50 mmol·L^-1	100 mmol·L^-1	200 mmol·L^-1
藏青16	231.42±12.99 a	153.88±17.14 b	43.27±9.36 c	5.17±4.01 d
藏青17	223.61±24.50 a	142.13±12.46 b	36.41±9.43 c	4.26±4.11 d
藏青148	236.62±14.05 a	187.68±19.40 b	64.08±8.52 c	11.58±4.71 d
藏青320	244.36±13.79 a	200.07±14.62 b	94.52±12.45 c	32.18±5.90 d
藏青2000	228.73±14.71 a	183.83±16.16 b	75.10±11.01 c	23.63±5.36 d
藏青3000	209.61±14.64 a	119.83±14.82 b	27.69±8.31 c	5.13±2.95 d
山青9号	231.82±12.91 a	168.58±17.68 b	56.98±10.50 c	23.51±2.87 d
喜拉19	226.64±12.74 a	159.32±16.11 b	67.88±11.24 c	23.55±5.13 d

Screening of Salt-tolerant, Low-Cr-accumulating Hulless Barley Varieties and Their Growth Response Characteristics

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品种	盐浓度/（mmol·L^-1）	根长/cm	芽长/cm	鲜重/mg	干重/mg	含水量/%
藏青16	0	8.40±0.46	8.77±1.06	213.00±11.09	23.90±1.44	88.80±0.79
	50	7.94±0.69	7.50±0.90	178.60±10.82	20.30±2.01	88.61±0.89
	100	3.17±0.65	2.63±0.45	53.60±1.47	6.90±0.26	87.16±1.11
	200	1.17±0.86	0.93±0.07	24.20±1.06	3.40±0.28	85.79±0.68
藏青17	0	8.23±0.89	7.81±1.18	209.50±10.13	23.70±1.56	88.66±0.99
	50	7.67±0.59	6.87±0.28	161.40±10.14	20.80±1.52	87.10±0.72
	100	2.96±0.70	2.49±0.27	51.60±3.18	6.70±0.41	87.05±0.49
	200	0.82±0.78	0.67±0.06	21.50±1.09	2.80±0.23	86.76±1.27
藏青148	0	8.53±0.43	8.32±0.57	207.00±7.32	22.50±0.97	89.12±1.22
	50	8.00±0.75	7.33±0.76	182.80±15.53	21.80±1.63	88.09±1.03
	100	3.59±0.39	2.91±0.23	63.70±3.05	8.30±0.55	86.97±0.72
	200	1.68±0.65	1.21±0.15	29.40±1.63	4.10±0.42	85.95±1.06
藏青320	0	8.69±0.45	8.62±0.84	214.40±11.61	25.50±2.16	88.10±0.76
	50	8.23±0.55	7.61±0.53	183.80±11.53	21.00±1.67	88.58±0.71
	100	4.81±0.58	4.01±0.42	82.40±3.82	10.60±0.80	87.12±1.11
	200	3.64±0.62	2.92±0.17	43.80±2.46	6.10±0.33	86.17±1.29
藏青2000	0	8.36±0.46	8.26±0.65	210.50±10.84	23.00±1.34	89.07±0.98
	50	8.07±0.56	7.37±0.69	184.80±12.93	21.10±1.43	88.58±0.92
	100	4.47±0.53	3.58±0.34	76.30±4.52	10.00±0.77	86.89±0.92
	200	3.03±0.67	2.42±0.20	39.50±2.34	5.60±0.42	85.75±1.09
藏青3000	0	7.88±0.54	7.57±0.84	192.6±9.67	22.30±1.61	88.44±1.15
	50	7.12±0.67	6.16±0.54	150.00±9.63	18.20±1.32	87.88±0.75
	100	2.60±0.73	2.18±0.31	46.60±3.07	6.10±0.43	86.84±0.91
	200	1.36±0.64	0.90±0.09	25.00±1.55	3.60±0.34	85.59±0.92
山青9号	0	8.30±0.43	8.24±0.73	212.30±9.07	23.50±1.46	88.93±0.94
	50	7.87±0.56	7.18±0.53	179.10±11.33	21.20±1.64	88.18±0.88
	100	3.55±0.62	2.90±0.37	62.50±3.16	8.20±0.61	86.87±0.73
	200	2.74±0.31	2.06±0.15	35.50±2.03	5.10±0.47	85.67±0.84
喜拉19	0	8.17±0.42	8.12±0.63	207.70±10.48	22.50±1.29	89.19±0.91
	50	7.81±0.53	7.14±0.52	176.70±11.59	20.90±1.40	88.19±0.87
	100	4.31±0.67	3.44±0.46	72.80±4.22	9.70±0.83	86.66±0.99
	200	3.07±0.58	2.39±0.19	39.80±2.15	5.70±0.43	85.64±0.90

青稞品种	铬浓度/(mg·L^-1)	鲜重/g		干重/g		含水量/%
青稞品种	铬浓度/(mg·L^-1)	根	地上部	根	地上部	根	地上部
藏青148	0	0.214±0.04	0.404±0.08	0.023±0.004	0.047±0.009	89.15±2.74	88.34±3.20
	10	0.060±0.02	0.090±0.04	0.007±0.002	0.010±0.005	88.16±5.12	88.69±7.43
	50	0.062±0.02	0.027±0.01	0.009±0.003	0.008±0.003	86.28±6.73	72.14±5.62
藏青320	0	0.289±0.08	0.332±0.05	0.031±0.009	0.042±0.006	89.31±4.30	87.26±2.63
	10	0.064±0.01	0.062±0.02	0.012±0.002	0.015±0.005	80.71±4.28	76.24±11.22
	50	0.033±0.01	0.024±0.01	0.006±0.002	0.007±0.003	80.50±8.19	70.75±7.43
藏青2000	0	0.082±0.03	0.147±0.08	0.009±0.003	0.019±0.010	89.31±5.43	86.97±9.79
	10	0.075±0.01	0.073±0.01	0.008±0.001	0.012±0.002	88.98±1.95	84.25±3.55
	50	0.042±0.01	0.032±0.01	0.006±0.001	0.012±0.004	86.59±4.15	63.73±7.13
山青9号	0	0.125±0.07	0.217±0.08	0.013±0.007	0.031±0.012	89.63±8.08	85.58±7.64
	10	0.085±0.03	0.059±0.07	0.010±0.003	0.013±0.006	88.59±5.45	78.72±8.06
	50	0.069±0.02	0.038±0.05	0.008±0.002	0.010±0.004	88.72±4.44	73.11±7.89
喜拉19	0	0.133±0.04	0.214±0.06	0.017±0.005	0.029±0.008	87.32±5.38	86.49±5.33
	10	0.043±0.01	0.038±0.01	0.008±0.002	0.008±0.002	82.02±6.35	77.68±7.64
	50	0.045±0.01	0.034±0.01	0.009±0.002	0.012±0.004	80.05±6.29	64.57±5.69

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