Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109

doi:10.19586/j.2095-2341.2026.0057

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (4): 919-925.DOI: 10.19586/j.2095-2341.2026.0057

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Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109

Hongqi ZHANG¹(), Yuehua YU²(), Zhiyong NI¹()

^1.Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms，College of Life Sciences，Xinjiang Agricultural University，Urumqi 830052，China
^2.College of Agronomy，Xinjiang Agricultural University，Urumqi 830052，China

Received:2026-03-06 Accepted:2026-04-15 Online:2026-07-25 Published:2026-09-11
Contact: Yuehua YU,Zhiyong NI

Abstract

Abstract:

To investigate the function of the GmMYB109 transcription factor in soybean， we performed bioinformatics analysis on its sequence， examined its subcellular localization using transient expression in tobacco， and determined its expression levels in different tissues and at different time points under salt stress by qRT?PCR. Bioinformatics prediction showed that GmMYB109 contains two SANT domains and is localized in the nucleus. Transient expression assays confirmed its nuclear localization in tobacco epidermal cells， indicating that GmMYB109 is a nuclear?localized transcription factor. qRT?PCR results revealed that GmMYB109 was most highly expressed in leaves， followed by stems， cotyledons and roots. Its expression gradually increased under salt stress， suggesting that GmMYB109 is induced by salt stress. These results provide theoretical basis for further exploring the biological function and regulatory mechanism of the GmMYB109 transcription factor.

Key words: soybean, MYB transcription factor, subcellular localization, salt stress

CLC Number:

Q291

Hongqi ZHANG, Yuehua YU, Zhiyong NI. Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109[J]. Current Biotechnology, 2026, 16(4): 919-925.

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引物名称	引物序列（5'→3'）	用途
GmMYB109-F	5'-ATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	基因克隆
GmMYB109-R	5'-5'-TTATTGATGCATTGTTGGAGGTGCCAT-3'	基因克隆
GmMYB109-pCAMBIA1304-F	5'-cgggggactcttgaccatggATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	亚细胞定位
GmMYB109-pCAMBIA1304-R	5'-ctcctttactagtcagatctTTGATGCATTGTTGGAGGTGCCAT-3'	亚细胞定位
GmMYB109-qPCR-F	5'-AGTTGGACATCCTGTGGCTG-3'	荧光定量
GmMYB109-qPCR-R	5'-GATGAGGATGAGCATGGGGG-3'	荧光定量
GmCYP2-F	5'-CGGGACCAGTGTGCTTCTTCA-3'	荧光定量内参引物
GmCYP2-R	5'-CCCCTCCACTACAAAGGCTCG-3'	荧光定量内参引物

引物名称	引物序列（5'→3'）	用途
GmMYB109-F	5'-ATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	基因克隆
GmMYB109-R	5'-5'-TTATTGATGCATTGTTGGAGGTGCCAT-3'	基因克隆
GmMYB109-pCAMBIA1304-F	5'-cgggggactcttgaccatggATGTCATCAAGTGGAACCATTTGGAGCTAT-3'	亚细胞定位
GmMYB109-pCAMBIA1304-R	5'-ctcctttactagtcagatctTTGATGCATTGTTGGAGGTGCCAT-3'	亚细胞定位
GmMYB109-qPCR-F	5'-AGTTGGACATCCTGTGGCTG-3'	荧光定量
GmMYB109-qPCR-R	5'-GATGAGGATGAGCATGGGGG-3'	荧光定量
GmCYP2-F	5'-CGGGACCAGTGTGCTTCTTCA-3'	荧光定量内参引物
GmCYP2-R	5'-CCCCTCCACTACAAAGGCTCG-3'	荧光定量内参引物

Analysis of Subcellular Localization and Expression Patterns of Soybean Transcription Factor GmMYB109

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