Screening and Functional Enrichment Analysis of Key Hub Genes in Lynch Syndrome-associated Colorectal Cancer Based on Bioinformatics Analysis

doi:10.19586/j.2095-2341.2022.0122

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (2): 291-297.DOI: 10.19586/j.2095-2341.2022.0122

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Screening and Functional Enrichment Analysis of Key Hub Genes in Lynch Syndrome-associated Colorectal Cancer Based on Bioinformatics Analysis

Xingshu ZHU¹(), Junjie HUANG¹, Caiming WENG², Wangwu LIU², Yongyuan CHEN², Huiyan CHEN², Hu ZHAO²^,³, Yu WANG²^,³, Chengzhi LIN²^,³()

^1.College of Integrated Medicine，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China
^2.Fuzhou General Clinical Medical College of Fujian Medical University （900TH Hospital），Fuzhou 350025，China
^3.900TH Hospital of Joint Logistics Support Force，Fuzhou 350025，China

Received:2022-07-01 Accepted:2022-08-18 Online:2023-03-25 Published:2023-04-07
Contact: Chengzhi LIN

Abstract

Abstract:

To screen the differentially expressed genes （DEGs） in Lynch syndrome related colorectal cancer （CRC） by various bioinformatic analysis. The dataset GSE178516 was downloaded from gene expression omnibus （GEO）. DEGs were screened by employing the GEO2R tool， and then intersected with the genes related to CRC and Lynch syndrome from the MalaCards to obtain the common differential genes. Then the functional enrichment analysis and protein-protein interaction （PPI） network was constructed by using String and Cytoscape software. Finally， expressions of the hub genes were verified by easyGEO database. A total of 261 differentially expressed genes were identified， consisting of 117 up-regulated genes and 114 down-regulated genes. After intersection with CRC and Lynch syndrome related genes， a total of 8 genes （TGFBR2， PTEN， POLD1， EXO1， EPCAM， CDKN2B， CCND1， BRCA1） were obtained. GO and KEGG enrichment analysis showed that their functions were mainly enriched in DNA metabolic process regulation， intrinsic apoptosis signaling pathway， mismatch repair， etc. Through PPI analysis， 892 proteins interacting with 8 common DEGs were obtained， including 44 nodes and 630 node interaction relationships. Finally， all 8 genes were statistically differentially expressed in patients with CRC associated with Lynch syndrome. The key Hub genes identified in this study would help us understand the molecular mechanisms behind the occurrence and development of Lynch syndrome combined with CRC， and ultimately provide new candidate targets for the diagnosis and treatment of Lynch syndrome progression to CRC.

Key words: Lynch syndrome, colorectal cancer, differentially expressed gene, Hub gene, bioinformatics

CLC Number:

Q811.4

Xingshu ZHU, Junjie HUANG, Caiming WENG, Wangwu LIU, Yongyuan CHEN, Huiyan CHEN, Hu ZHAO, Yu WANG, Chengzhi LIN. Screening and Functional Enrichment Analysis of Key Hub Genes in Lynch Syndrome-associated Colorectal Cancer Based on Bioinformatics Analysis[J]. Current Biotechnology, 2023, 13(2): 291-297.

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Figures/Tables 4

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Screening and Functional Enrichment Analysis of Key Hub Genes in Lynch Syndrome-associated Colorectal Cancer Based on Bioinformatics Analysis

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