journal1 ›› 2012, Vol. 2 ›› Issue (3): 190-194.DOI: 10.3969/j.issn.2095-2341.2012.0 .

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Identification and Distribution of Endophytic Fungi in Some Wild Dendrobia Plants Grown in Dehong, Yunnan

  

  1. 1.Key Lab for Agrobiodiversity and Plant Disease management, Ministry of Education; Yunnan Agricultural University, Kunming 650201, China;
    2.Scientific and Technology Division, Yunnan Agricultural University, Kunming 650201, China;
    3.College of Agronomy and Biotechnolog, Yunnan Agricultural University, Kunming 650201, China
  • Received:2011-02-27 Online:2012-05-25 Published:2012-03-23
  • Supported by:

    国家自然科学基金项目(31160352,30660006)资助。

几种云南野生石斛内生真菌的鉴定及分布

洪群艳1§,董文汉2§,徐文婷3,杨根华1*,梁泉3,浦锦月3,秦蔚1   

  1. 1.云南农业大学农业生物多样性与病虫害控制教育部重点实验室, 昆明 650201;
    2.云南农业大学科技处, 昆明 650201;
    3.云南农业大学农学与生物技术学院, 昆明 650201
  • 通讯作者: 杨根华,教授,博士,从事植物病理和微生物资源开发利用研究。E-mail:ghyang2003@126.com
  • 作者简介:洪群艳,硕士,主要从事植物病理学方面的研究。E-mail:569969211@qq.com。董文汉,副教授,主要从事植物病理学方面的研究。E-mail:whdong2004@yahoo.com.cn

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Abstract: Forty-three strains of endophytic fungi were isolated from the roots, stems and leaves of the 8 different wild Dendrobia plants. After 5.8S rDNA sequence analysis, these strains were clarified to 13 genera including Colletotrichum、Fusarium、Gibberella、Glomerella、Hypocrea、Nectria、Phomopsis、Phoma、Trichoderma and some others. In all the fungi,Colletotrichum were isolated most frequently from stems and leaves and the isolation rate was accounted for 39.5%, while Hypocrea, Nectria and Gibberella strains were firstly isolated from wild Dendrobia plants. It was also demonstrated that the endophytic fungi highly diverse depending up Dendrobia plants. Furthermore, the distribution of endophytic fungi highly varied from roots, stems and leaves. This research has got abundant resources of endophytic fungi.

Key words: dendrobium, endophytic fungi, 5.8S rDNA sequence analysis

摘要： 采用组织分离法,从采自德宏州的8种野生石斛根、茎、叶中分离得到内生真菌43株,经分子生物学鉴定分别归属于13个属。Colletotrichum为茎和叶的优势种群,占总菌株数的39.5%。Hypocrea、Nectria、Gibberella三个属内生真菌首次从石斛植物体内分离得到。研究发现,不同石斛植物内生真菌的存在情况差别较大,具有一定的宿主差异性;根、茎、叶内生真菌分布存在差异,表明内生真菌定殖具有一定的组织差异性和组织专一性。本研究获得丰富的内生真菌资源,为石斛的人工栽培提供基础的理论依据。

关键词: 石斛, 内生真菌, 分子鉴定

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