Table of Content

• 
• Current Biotechnology
  Volume 15 Issue 3 25 May 2025

Reviews

• Research and Application Progress of Omics Technology in Meat Quality Evaluation
• Haojie LI, Xinlu LI, Kun WANG, Changrong GE
• 2025, 15(3):  365-371.  DOI: 10.19586/j.2095-2341.2024.0193
• Abstract ( ) HTML ( ) PDF (512KB)( )

As an important part of human diet， meat is becoming more and more important with the development of social economy and the improvement of living standard. At present， consumers' demand for meat products is no longer limited to basic nutrition and hygiene requirements， they pay more attention to the health， taste and flavor of products， and the omics technology provides scientific means for meat quality evaluation （meat color， pH， water holding capacity， tenderness， free amino acids， intramuscular fat， etc.）. The review introduced the concept and research strategies of omics technology， summarized the application progress of omics technology in meat quality evaluation， in order to provid reference for further improving meat quality by using omics technology.

• Research Advances on the Mechanism of Root Suckering in Poplar
• Wanwan LYU, Lin ZHENG, Hongying PANG, Hongbo GAO, Hongzhi WANG
• 2025, 15(3):  372-379.  DOI: 10.19586/j.2095-2341.2025.0001
• Abstract ( ) HTML ( ) PDF (2121KB)( )

White poplar （Populus sect. Leuce） is an important poplar species widely cultivated in China. However， most of its varieties exhibit difficulties in cutting rooting， which limits the application of superior white poplar clones in breeding and the large-scale propagation of high-quality cultivars. Root suckers， characterized by rapid growth and high survival rates， have become a key technique for clonal propagation and afforestation of elite white poplar varieties. Currently， the molecular mechanism of root sucker formation in poplars remains not to be clarified. This review systematically summarized the morphological characteristics of root sucker formation in poplars and the factors influencing this process， and explored potential molecular signaling pathways and gene regulatory networks involved in root sucker initiation. The article not only provided theoretical support for developing precision cultivation and management strategies to enhance the efficiency of clonal propagation via root suckers， but also offered novel insights into the molecular regulatory mechanisms of root sucker formation， which is of great significance for advancing forest genetic improvement and the breeding of superior tree varieties.

• Research Progress on Yeast Expression of Therapeutic Recombinant Proteins
• Zhaohui CUI, Ling GUO, Xudong SHEN, Yi LIN, Lili ZHAI
• 2025, 15(3):  380-387.  DOI: 10.19586/j.2095-2341.2025.0045
• Abstract ( ) HTML ( ) PDF (826KB)( )

Therapeutic recombinant proteins， characterized by their high specificity， safety and minimal adverse reactions， play a crucial role in the clinical treatment of various diseases. Yeast， as a single-celled eukaryotic microorganism， has become the preferred host for expressing therapeutic recombinant proteins due to its similarity in protein secretion pathways with higher eukaryotes. However， bottlenecks remain in both the quality and production efficiency of yeast-expressed therapeutic recombinant proteins. The review summarized the latest advances in synthetic biology approaches to construct yeast cell with enhanced protein folding and secretion capabilities， with a focus on advanced cellular engineering strategies， including endoplasmic reticulum （ER） and protein trafficking pathway engineering， and humanization of glycosylation modification， in order to provide reference for improving the quality and yield of therapeutic recombinant proteins.

• Research Progress on Coupled Drugs
• Mingming YANG, Hui WANG
• 2025, 15(3):  388-395.  DOI: 10.19586/j.2095-2341.2025.0008
• Abstract ( ) HTML ( ) PDF (579KB)( )

The clinical success of antibody-drug conjugates （ADCs） has driven the rapid development of novel conjugate drugs， including radionuclide drug conjugates （RDCs）， peptide drug conjugates （PDCs）， and small molecule drug conjugates （SMDCs）. These drugs offer significant advantages in cancer treatment by integrating targeted delivery with efficient payload release and are progressively expanding into disease diagnosis and therapeutic areas beyond oncology. This article systematically reviewed the current landscape of conjugate drugs， coupling technologies， recent progress， and clinical translation， while exploring future directions in expanding indications， technological advancements， and integrated theranostic applications.

• Progress on the Development of Vaccines Related to Chronic Respiratory Diseases
• Yanhua LIU, Rui GUO, Yanyi LI, Jinli CHEN
• 2025, 15(3):  396-403.  DOI: 10.19586/j.2095-2341.2025.0003
• Abstract ( ) HTML ( ) PDF (535KB)( )

Chronic respiratory diseases （CRD） are the leading cause of death for over 4 million people each year worldwide. Although current treatments help manage daily symptoms and improve patients' quality of life， the acute exacerbations primarily triggered by respiratory infections still need prevention. Therefore， CRD patients are the main target of vaccination against infectious diseases. In this article， we reviewed the latest technologies available for vaccines specifically indicated for CRD patients， including respiratory syncytial virus vaccines， SARS-CoV-2 vaccines for pneumococcal infections， influenza， pertussis vaccines， and more， which was expected to provide reference for the prevention strategies for chronic respiratory diseases.

• GLP-1 Receptor Agonists: Emerging Therapeutic Strategy for Parkinson‍'‍s Disease
• Yanhua LIU, Rui GUO, Yanyi LI, Jinli CHEN
• 2025, 15(3):  404-410.  DOI: 10.19586/j.2095-2341.2025.0021
• Abstract ( ) HTML ( ) PDF (484KB)( )

Parkinson's disease （PD） is one of the most common neurodegenerative diseases. The latest research data reveal that neurodegenerative diseases and type 2 diabetes （T2DM） may have similar pharmacological mechanisms. Glucagon like peptide-1 receptor （GLP-1R） agonists are widely known for their successful application in the treatment of T2DM and are currently studied as a novel PD therapeutic drug. This review summarized the possible co-pathogenic pathways between T2DM and PD， and discussed the potential mechanism of action of GLP-1R agonists as targets for PD treatment， in order to provide theoretical support for the development of more effective PD treatment plans.

• Research Progress of siRNA Gene Therapy in Tendon Injury Repair
• Bingyue QIU, Jiateng SHI, Jing JIN
• 2025, 15(3):  411-417.  DOI: 10.19586/j.2095-2341.2024.0196
• Abstract ( ) HTML ( ) PDF (502KB)( )

Tendon injury is one of the most common symptoms in trauma surgery， which is mainly treated by surgical suturing in clinic. However， surgical treatment remains complications of re-reputure and adhesion formation after operation. In recent years， multiple nucleic acid drugs based on RNA interference （RNAi） have been on the market and gene therapy has received widespread attention. It is critical to choose a kind of suitable carrier to avoid the degradation of small interfering RNA （siRNA） and escape the endonucleases. Compared to the viral vectors that are prone to mutations， nanoparticles have higher safety and modifiability. This review systematically summarized the mechanism of action of siRNA， optimization strategies for gene therapy vectors， and their application progress in tendon repair， and looks forward to the potential of nanocarriers in clinical translation.

• The Research Progress on the Relationship Between Autism and the Three-dimensional Structure of Chromosomes
• Haiming GAO, Yuanyuan ZHANG, Rong HE
• 2025, 15(3):  418-425.  DOI: 10.19586/j.2095-2341.2024.0206
• Abstract ( ) HTML ( ) PDF (749KB)( )

Autism spectrum disorder （ASD） is a complex neurological disorder with an incidence rate of 1% to 2%， characterized by significant genetic heterogeneity. Three-dimensional genomics research has revealed the core role of dynamic regulation of chromatin spatial topology in the pathological mechanism of ASD， especially the disruption of boundaries or internal interactions within topologically associated domains （TADs）， which could lead to spatiotemporal expression dysregulation of key neurodevelopmental genes through epigenetic reprogramming. However，ASD-related three-dimensional genomic variations related has not been systematically clarified and the existing clinical diagnostic system lacks the integrated application of three-dimensional genomic markers. The article systematically reviewed the molecular mechanisms of TAD-mediated chromatin compartmentalization regulation in ASD neurodevelopment and summarized the technological innovations of high-throughput chromosome conformation capture technology （Hi-C） and its derivative methods in the construction of three-dimensional epigenomic maps of ASD， in order to provide theoretical support and technical paradigms for the early molecular typing and targeted treatment of ASD.

• The Novel Role and Research Advances of the Gut in Obesity-related Hypertension
• Ling SHU, Mingwei BAO
• 2025, 15(3):  426-431.  DOI: 10.19586/j.2095-2341.2024.0166
• Abstract ( ) HTML ( ) PDF (496KB)( )

Obesity related hypertension （OH） is a subtype of hypertension primarily caused by obesity and excluding other secondary hypertension. Its blood pressure levels can be significantly reduced through weight management in OH patients. OH is complex and involves multiple system interactions， mainly including enteroendocrine dysregulation， sodium-sensing receptor dysfunction， intestinal barrier impairment，and gut microbiota dysbiosis. Recent studies have shown that the gastrointestinal tract may play a key modulator in early OH pathogenesis and may even be regarded as a potential therapeutic target. From the dual perspectives of energy metabolism regulation and blood pressure homeostasis imbalance， the article systematically reviewed the pathophysiological role of the gastrointestinal tract in OH， in order to provide reference for the treatment of hypertension.

Articles

• Integrating GWAS and Transcriptome Profiling to Identify SNP Markers Linked to High-temperature Tolerance in Esox lucius
• AYELHAN·Haysa, Fei JIAO, Hong LIU, ANASI·Hudelati, Yubang SHEN
• 2025, 15(3):  432-445.  DOI: 10.19586/j.2095-2341.2024.0184
• Abstract ( ) HTML ( ) PDF (10611KB)( )

To enhance the extreme high-temperature adaptability of Esox lucius for aquaculture expansion to southern warm regions and identify genetic loci associated with thermal tolerance， we integrated genome-wide association study （GWAS） and transcriptome analysis. Using reduced-representation genome sequencing data from heat-sensitive and heat-tolerant populations， GWAS analysis with an MLM model identified 471 SNPs significantly associated with thermal tolerance traits. Concurrently， transcriptome sequencing of juvenile brain tissues exposed to varying heat stress durations revealed differential gene expression patterns. Functional enrichment analyses demonstrated significant enrichment of differentially expressed genes （DEGs） in endoplasmic reticulum stress and immune-related pathways. GSEA analysis indicated activation of innate immune responses at 84 h of heat stress， with specific induction of NAD（+） ADP-ribosyltransferase activity-related genes. Protein-protein interaction （PPI） network analysis identified parp14， parp9， stat1， and stat3 as core regulators of innate immune responses. Notably， two GWAS-derived SNPs located in exon 3 of the parp14 gene—rsc.646T>C （missense mutation） and rsc.777G>A （synonymous mutation）—exhibited complete linkage disequilibrium and were significantly correlated with thermal tolerance traits. Sanger sequencing validation in heat-stressed and reference populations confirmed these associations. Our findings reveal candidate genes and functional SNP markers for high-temperature tolerance in E. lucius， providing insights into its genetic mechanisms and supporting marker-assisted breeding strategies.

• Studies on the Immunological Effects of Dinotefuran Exposure on Zebrafish Juveniles
• Yunshuo CHENG, Zixu LI, Guanghua MAO, Xiangyang WU
• 2025, 15(3):  446-455.  DOI: 10.19586/j.2095-2341.2025.0015
• Abstract ( ) HTML ( ) PDF (2850KB)( )

In order to investigate the immunotoxicity and mechanism of action of dinotefuran （DIN） on early developmental stages of zebrafish， zebrafish larvae were exposed to different concentrations of DIN （2， 200 and 2 000 μg?L^-1） for 5 days to investigate changes in oxidative stress， immune cells， immune-related parameters and immune-related pathways. The results demonstrated that DIN significantly reduced the number of neutrophil， macrophage and thymic T cells （P<0.01）， decreased the levels of immune factors （LYS， IgM and C3） （P<0.01）， and increased the levels of inflammatory factors （IL-1β， IL-6， and TNF-α） （P<0.01）. DIN dose-dependently increased the levels of reactive oxygen species （ROS） in juvenile fish （P<0.01） and inhibited antioxidant enzyme activities （CAT， SOD and GSH-Px） （P<0.01）. Concurrently， DIN exposure modulated the transcript levels of pivotal genes within the TLR4/NF-κB， JAK-STAT and Nrf2-Keap1 signalling pathways. The study demonstrated that DIN exposure could induce immunotoxicity in zebrafish larvae and that the TLR4/NF-κB， JAK-STAT and Nrf2-Keap1 pathways play a significant role in this process.

• Risk Identification and Suggestions for the Industrialization of Biological Breeding——based on Grower Field Research
• Ziya GAO, Bingxin HUANGFU, Jiake LI, Fangqian LI, Zhihao MA, Yang PANG, Jingang LIANG, Xiaoyun HE
• 2025, 15(3):  456-465.  DOI: 10.19586/j.2095-2341.2024.0203
• Abstract ( ) HTML ( ) PDF (3032KB)( )

Crop cultivation is a critical component of the food supply chain， and establishing appropriate legal oversight and risk prevention/control systems for this stage is essential to advancing the industrialization of biological breeding crops. Through field surveys of growers， logistic regression， and correlation analysis， this study identified significant correlations between growers' support for biological breeding industrialization and their basic characteristics， including geographic location， cultivated land area， educational attainment， annual household income， internet access， and attitudes toward adopting new crop varieties. However， growers demonstrated limited awareness of biological breeding industrialization and inadequate risk management capabilities. By evaluating growers' acceptance levels and the operational efficiency of biological breeding practices， this research pinpointed key risks in four areas： economic viability， field management， post-harvest storage， and public perception. Finally， risk management strategies were proposed， categorized by responsible stakeholders and prioritized for risk mitigation and prevention.

• Development of PCR Qualitative Method for Genetically Modified Herbicide-tolerant Soyabean LP012-1
• Yueqiu ZHANG, Fangqi FU, Tongtong ZHAO, Ziyan CHEN, Xinyao PEI, Fangrui GAO, Jiake LI, Hui LI, Xiaodan JIANG, Haoqian WANG, Hong CHEN
• 2025, 15(3):  466-475.  DOI: 10.19586/j.2095-2341.2024.0207
• Abstract ( ) HTML ( ) PDF (3255KB)( )

Genetically modified herbicide-tolerant soybean transformant LP012-1 was independently developed by Chinese enterprises and has broad application prospects with biological breeding industrialization. By establishing a qualitative detection method for LP012-1 transformants， strong support can be provided for label management and safety supervision. Based on this， a general PCR qualitative detection method for LP012-1 transformants was constructed from optimizing DNA extraction methods， designing transformant specific primers， optimizing primer concentration and annealing temperature， verifying method specificity， testing detection limits， and its robustness. The establishment of a highly sensitive and specific LP012-1 transformant detection method fills the gap in qualitative detection methods for this transformant， and can provide support for standardized management and promotion of application in the later stage.

• Bibliometric Analysis of Nucleotide-based Detection Methods for Genetically Modified Plants and Derived Products
• Zhi WANG, Guang HU, Wei FU, Xuanzi PENG, Yongjiang ZHANG, Junfeng ZHAI
• 2025, 15(3):  476-485.  DOI: 10.19586/j.2095-2341.2024.0160
• Abstract ( ) HTML ( ) PDF (2162KB)( )

With the expanding variety and quantity of genetically modified （GM） plants and their derived products， requirements for nucleic acid detection precision have become increasingly stringent. This study utilized the Web of Science Core Collection database， employing bibliometric methods to comprehensively analyze research trends， primary directions， global research capacity distribution， and China’s scientific standing in GM nucleic acid detection technology. The analysis results showed that as of early 2023， the total number of SCI related to nucleic acid testing technology has reached 2 550， and China ranks first in the global publication volume in this field. More than 90 countries/regions and approximately 2 400 institutions worldwide have participated in the research of GM nucleic acid testing technology. Among the top 20 institutions in terms of publication volume， 7 of them are from China. Among the 30 high-quality journal articles related to GM nucleic acid detection technology， 14 are from China， indicating that relevant research in China has shown a trend of high-quality development. At the same time， this article summarized the current status of different GM nucleic acid testing technologies from multiple dimensions such as paper publication trends， publication volume， and publishing institutions， and analyzed the differences in research trends of the above technologies， aiming to ensure the efficient utilization of scientific research resources and provide data support for regulatory agencies’ strategic decision-making and research directions.

• The Evaluation Method for Drought Resistance During the Germination Period of Upland Cotton and Screening of High-quality Germplasm Resources in the Shihezi Reclamation Area
• Jingjing YANG, Kewei NING, Mingjuan WANG, Binghui TANG, Junling CAI, Aiping LIU, Xiaodong XIE
• 2025, 15(3):  486-494.  DOI: 10.19586/j.2095-2341.2024.0188
• Abstract ( ) HTML ( ) PDF (538KB)( )

To investigate the relationship between morphological indicators during the germination stage and drought resistance in upland cotton （Gossypium hirsutum L.）， and to identify elite germplasm resources with superior drought tolerance， this study applied polyethylene glycol-6000 （PEG-6000） to simulate drought stress through hypertonic solution， with distilled water as the control. A systematic drought resistance evaluation was performed on 34 cotton varieties （lines） from the Shihezi Reclamation Area. The results indicated that three evaluation methods （the Petri dish filter paper germination method for drought resistance assessment， the germination box sand culture method for drought resistance assessment， and the beaker dual-concentration filter paper method for drought resistance assessment） could effectively identify the drought resistance of cotton during the germination stage. Through comprehensive assessment using membership function analysis， the relative germination vigor， relative germination rate， and relative germination index emerged as key indicators for rapid evaluation， with 44.1% （15 varieties） exhibiting strong drought tolerance. According to the industry standard for germination-stage drought resistance， 32.4% （11 varieties） showed extreme drought tolerance. Using the dual-concentration PEG-6000 method， 11.8% （4 varieties） achieved Level 1 drought resistance classification. All three methods consistently identified four varieties—Xinluzao 79， Xinluzao 84， H16， and Xinzaomian 107—as having exceptional drought resistance. Considering methodological universality and operational simplicity， the dual-concentration PEG-6000 evaluation method is recommended as the preferred approach for drought resistance assessment in upland cotton.

• Optimization of Agrobacterium-mediated Transformation in Cotton Embryonic Tips Through Auxiliary Method
• Xuan DING, Zuying ZHANG, Xiujuan SU, Wenran HU
• 2025, 15(3):  495-501.  DOI: 10.19586/j.2095-2341.2024.0201
• Abstract ( ) PDF (2150KB)( )

The genetic transformation of cotton is constrained by multiple limiting factors， including genotype restrictions and protracted transformation cycles， resulting in low transformation efficiency. In this study， embryonic tips of upland cotton TM-1 were selected as recipient material for Agrobacterium-mediated transformation using a GhTCP4 gene editing vector. We systematically investigated the effects of different concentrations of exogenous hormones 6-benzylaminopwrine （6-BA） and naphthalene acetic acid （NAA） in plant regeneration media， ultimately determining that hormone-free medium yielded optimal recovery. Furthermore， three auxiliary treatments， ultrasonication， vacuum infiltration， and their combination， were evaluated for their impacts on transformation efficiency. The results demonstrated that 15 minute ultrasonic treatment at 40 kHz frequency with 300 W rated power achieved the highest transformation efficiency of 26.86%. This study provided methodological insights for establishing an efficient cotton embryonic tip transformation system and offered a foundation for future functional gene research and genetic transformation applications in cotton.

• Analysis and Composition Characteristics of Essential Oils of Different Peppermint Varieties
• Lin LU, Hua CAO, Jun YANG, Guolin SHEN, Han LI
• 2025, 15(3):  502-509.  DOI: 10.19586/j.2095-2341.2024.0140
• Abstract ( ) HTML ( ) PDF (1323KB)( )

To investigate the essential oil content and compositional characteristics of different peppermint varieties， 25 collected peppermint varieties resources were analyzed. The experiment utilized steam distillation to extraet peppermint essenitial oil， and analyzed components using gas chromatography-mass spectrometry （GC-MS）. The study compared the content and group characteristics of bioactive chemical constituents across these varieties. Results revealed that the oil content of 24 peppermint varieties ranged from 0.03% to 1.52%， with Gold Mint showing the highest content （1.52%）. A total of 53 volatile components were identified in 22 essential oils， with alcohols and terpenes universally present. Menthol was the predominant alcohol， reaching 76.45% in Gold Mint， while esters such as linalyl acetate （48.66% in Japanese Mint） were secondary major components. Terpene compounds， including β?-caryophyllene， germacrene D， limonene， and 1，8-cineole， were detected in 21 varieties. Variations in essential oil composition were observed among peppermint resources， yet these differences showed no correlation with genetic relatedness. Gold Mint emerged as the variety with both high oil yield and superior quality. This study clarifies the biological traits and essential oil profiles of peppermint， providing a scientific foundation for its utilization in essential oil applications.

• Antioxidant Activity and Composition Changes of Lycium chinense Miller Leaf Black Tea Fermented by Aspergillus cristatum
• Ju CHEN, Chunjie DING, Yinling SUN, Han ZHAO, Hongyu ZHENG, Xin GAO, Shuhan JIN, Kun DONG
• 2025, 15(3):  510-517.  DOI: 10.19586/j.2095-2341.2025.0005
• Abstract ( ) HTML ( ) PDF (1326KB)( )

To investigate the changes of active components in Lycium chinense Miller leaf drink fermented by Aspergillus cristatum and its effect on antioxidant activity in vitro， L. chinense Miller leaves（GY） from three distinct production regions in Zhangzhou， Fujian （FZ）， Qingyuan， Guangdong （GQ）， and Nantong， Jiangsu （JN） were harvested. According to the black tea process，L. chinense Miller leaves were withered， rolled and naturally fermented to make L. chinense Miller leaf black tea（GC）. The black tea was fermented by A. cristatum for 2 days to obtain Fermented L. chinense Miller leaf black tea （GCF）. Compared with GY， GCF could improve the taste of L. chinense Miller leaves and remove the grassy flavor； the color of tea soup deepened from orange yellow to orange red； there was no significant change in the content of polyphenols and carotenoids. The content of flavonoids decreased significantly， and the content of flavonoids in JNGCF decreased by 43.44%. Compared with each group of GY， the contents of protein， total polysaccharides and total free amino acids in FZGCF， GQGCF and JNGCF were significantly increased. The protein increased by 50.70%， 49.07% and 55.34%， respectively； the total polysaccharide increased by 98.93%， 113.15% and 100.42%； the total free amino acids increased by 75.90%， 82.38% and 68.84%. In the in vitro antioxidant activity test， the DPPH free radical scavenging rate of FZGCF was significantly reduced， while there was no significant difference between GQGCF， JNGCF and their respective GY groups， and the reducing power of FZGCF， GQGCF and JNGCF was significantly reduced. The results showed that GCF groups could improve the taste， increase the redness of tea soup， preserve the original antioxidant active substances polyphenols and carotenoids to a large extent， and increase the nutritional substances protein， polysaccharides， and amino acids. The above results provide a theoretical basis for the development of functional products of L. chinense Miller leaf， filling the gap in L. chinense Miller leaf black tea products and reducing the resource waste rate of L. chinense Miller leaf.

• Optimization of Fermentation Culture Conditions for Cyclosporine A Using Response Surface Method
• Xiaojing GAO, Zhulan ZHANG, Xianju LIN, Guanrong QIU, Yaoming WEN, Jianhui FAN, Hongxiang HUANG
• 2025, 15(3):  518-525.  DOI: 10.19586/j.2095-2341.2025.0006
• Abstract ( ) HTML ( ) PDF (2034KB)( )

Cyclosporin A （CsA） is a secondary metabolite with multiple biological activities such as immunosuppression， antibacterial， and anti-inflammatory effects， which holds significant application value in clinical treatment and the biomedical field. To further increase the fermentation content of CsA， based on single-factor experiments， the optimal fermentation conditions were obtained through Plackett-Burman design， central composite design （CCD） and response surface analysis （RSM）. The results indicated that the contents of corn steep powder， ammonium sulfate， glucose， and the fermentation time had a significant impact on the biosynthesis of CsA. Eventually， the suitable medium composition was determined as follows： 8.0% maltodextrin， 6.88% corn steep powder， 0.15% yeast extract powder， 0.18% ammonium sulfate， 0.61% glucose， and 0.1% PEG200. The optimized fermentation parameters were a cultivation temperature of 24 ℃ and a fermentation time of 10.8 days. The fermentation yield of CsA reached 11.76 mg·mL^-1， which was increased by 47.95% compared with the initial conditions， after the optimization of the fermentation process. The research results provide important theoretical basis and technical parameters for the process scale-up of this strain.

• The Otx2 Gene Regulates the Effect of H3K27me3 on the Wnt Pathway
• Yuanzhi ZHU, Pei PEI, Fan LIU, Xuejia HE, Yi WANG, Shan WANG
• 2025, 15(3):  526-534.  DOI: 10.19586/j.2095-2341.2024.0178
• Abstract ( ) PDF (1995KB)( )

Both Otx2 and the Wnt pathway are involved in nervous system development， and changes in H3K27me3 enrichment status are known to influence this process. This study investigated whether Otx2 regulates the Wnt pathway by modulating histone H3K27me3 modification. A stable C17.2 mouse neural stem cell line with Otx2 knockout was generated using CRISPR/Cas9. ChIP-Seq analysis identified DNA fragments bound by H3K27me3， and qPCR was used to assess Wnt-related gene expression changes after Otx2 knockout. GO and KEGG enrichment analyses revealed that differential genes associated with ChIP-Seq peaks between Otx2-knockout and control cells were predominantly enriched in pathways linked to nervous system development and function. Further analysis showed H3K27me3 enrichment at loci of Wnt5a， Wnt2， and Wnt16 genes in the Wnt pathway. Notably， Wnt5a， Wnt2， and Wnt5b expression levels were significantly reduced in Otx2-knockout cells compared to controls. These findings suggested that Otx2 regulates the Wnt pathway by altering H3K27me3 enrichment sites， providing mechanistic insights into neurological developmental abnormalities.

• The Role of SIRT1 Deacetylation Modification in Regulating HMGB1-mediated Pyroptosis in Chronic Sinusitis with Nasal Polyps
• Yu DING, Bo ZHAO, Jin ZHANG, Xudong GAO
• 2025, 15(3):  535-543.  DOI: 10.19586/j.2095-2341.2025.0002
• Abstract ( ) HTML ( ) PDF (4948KB)( )

To investigate the role and mechanism of sirtuin 1 （SIRT1）??-mediated deacetylation of high mobility group box 1 （HMGB1） in chronic rhinosinusitis with nasal polyps （CRSwNP）. CRSwNP cellular model was established using lipopolysaccharide （LPS）?-induced human primary nasal epithelial cells （HNEpC）. The effects of LPS on cell viability， expression of CRSwNP-related proteins （NLRP3， Caspase-1， TSLP）， SIRT1 expression， acetylation level and translocation of HMGB1， mRNA levels of inflammatory cytokines， and pyroptosis were examined. Furthermore， SIRT1 overexpression model was constructed or cells were treated with exogenous lactate to investigate the roles of SIRT1 and lactate in LPS-mediated pyroptosis. Compared with the control group， 30 μg·mL^-1 LPS had no effect on the viability of HNEpC cells but significantly increased the protein expression of NLRP3， Caspase-1， and TSLP， as well as the mRNA levels of inflammatory cytokines. Simultaneously， LPS significantly downregulated SIRT1 expression， increased HMGB1 acetylation and translocation， induced pyroptosis， and lactate production. Compared with the LPS-treated group， the SIRT1 overexpression group exhibited decreased HMGB1 acetylation and translocation， reduced release of inflammatory cytokines， inhibited pyroptosis， and significantly decreased lactate content in the supernatant. Exogenous lactate significantly downregulated SIRT1 protein expression， promoted HMGB1 acetylation and translocation， and enhanced the release of inflammatory cytokines and lactate. In summary， SIRT1 attenuates LPS-induced acetylation and translocation of HMGB1 in HNEpC， thereby ameliorating pyroptosis and reducing cellular inflammation.

• Genetic Polymorphism Analysis on Short Tandem Repeats of Y Chromosome in Han Population of Hohhot
• Luyao LI, Ning LYU, Yongjie ZHAI, Run ZHANG, Anxin YAN, Fuquan JIA
• 2025, 15(3):  544-554.  DOI: 10.19586/j.2095-2341.2024.0195
• Abstract ( ) HTML ( ) PDF (839KB)( )

To evaluate the genetic polymorphism of 38 Y-chromosomal short tandem repeat （Y-STR） loci in the Han population of Hohhot， Inner Mongolia Autonomous Region， and to explore their forensic application value and genetic relationships with other domestic populations， a multiplex amplification system comprising 38 Y-chromosomal genetic markers was used to amplify samples from 272 randomly selected unrelated Han male individuals in Hohhot City. Allele frequencies and forensic genetic parameters for the 38 Y-STR loci were calculated. Based on haplotype data from domestic populations included in the Y-chromosomal short tandem repeat haplotype reference database （YHRD）， pairwise genetic distances （Rst） between populations were computed， followed by multidimensional scaling （MDS） analysis. A total of 333 alleles were detected across the 38 Y-STR loci， with gene diversity （GD） values ranging from 0.043 4 to 0.967 5. In total， 269 haplotypes were observed， with three haplotypes occurring twice. Haplotype diversity （HD） and discrimination power （DC） were 0.999 9 and 0.989 0， respectively. Population comparison results revealed that the Hohhot Han population exhibited smaller genetic distances （indicating closer genetic relationships） with groups of the same ethnicity or geographically proximate populations， such as the Shaanxi Han population， and conversely， larger genetic distances （indicating more distant relationships） with other groups. The selected multiplex detection system containing 38 Y-STR loci demonstrated high genetic polymorphism， making it suitable for forensic analysis and population genetic studies of the Han population in Hohhot.