Table of Content

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• Current Biotechnology
  Volume 11 Issue 2 25 March 2021
• International Genetically Modified Food Safety Evaluation Policy and its Enlightenment
• JIAO Yue^1*, ZHU Pengyu², LIANG Jingang¹, FU Wei^2*
• 2021, 11(2):  121-127.  DOI: 10.19586/j.2095-2341.2020.0120
• Abstract ( ) PDF (1139KB)( )
As one of the cores of modern biotechnologies, transgenic technology has played an important role and shown great potential in guaranteeing food security, protecting ecological security and expanding agricultural functions. However, it also brings potential security problems. Therefore, major countries and international organizations have formulated rules, regulations and policies related to the safety assessment of genetically modified organisms (GMOs) to strengthen the management. The GMOs safety assessment systems and assessment policies of Codex Alimentarius Commission (CAC) and Organization for Economic Co-operation and Development (OECD) were mainly introduced and analyzed, and their actual positive effects and values were summarized, and their shortcomings or deficiencies were pointed out, in order to put forward thoughts on reference suggestions about theoretical policies and legal regulations for the improvement of the safety assessment system of genetically modified food and the improvement of regulatory measures in China.
• Application Progress of Loop-mediated Isothermal Amplification Technique
• LIU Wang, JIN Jinghao, CHEN Xiaoren
• 2021, 11(2):  128-135.  DOI: 10.19586/j.2095-2341.2020.0152
• Abstract ( ) PDF (1484KB)( )
Loop-mediated isothermal amplification (LAMP) is a new nucleic acid amplification technology, which relies on an active DNA polymerase with chain replacement activity and two pairs of specially designed primers to complete the amplification reaction efficiently and quickly under isothermal conditions. Compared with traditional amplification detection methods, LAMP technology has the advantages of strong specificity, high sensitivity, simple and rapid operation, and can be widely popularized in field rapid detection and grass-roots application. At present, LAMP technology has been widely used in the detection of plant diseases, animal diseases, food safety and other fields. Based on this, the basic principle of LAMP technology and the detection method of reaction products was briefly introduced, the improvement and development of LAMP technology was emphatically expounded, its application progress in scientific research and production in recent years was summarized, and its development prospect was looked forward, in order to provide a reasonable research direction for the further development of LAMP technology.
• Characters of Light-sheet Fluorescence Microscope and its Application
• CHEN Xuanyi¹, LI Shaojun^2*, CHEN Weimin², MENG Quanke³
• 2021, 11(2):  136-147.  DOI: 10.19586/j.2095-2341.2020.0115
• Abstract ( ) PDF (1784KB)( )
Conventional fluorescence microscopes are unable to meet the demand for prolonged observation of live samples with some certain fluorescence signal molecules due to their high phototoxicity, phtodamage, etc. As a novel fluorescence microscope, light sheet fluorescence microscope(LSFM) has outstanding characteristics which are different from laser scanning confocal microscope, such as the low phototoxicity, low photobleaching, low photodamage and the high spatiotemporal resolution due to its special orthogonal optical path design and efficient signal acquisition device, thus it can continuously record cells and large-scale biological tissue samples, especially for the live samples. Based on this, the imaging principle, imaging advantages, improvement and optimization process of imaging effect of LSFM and its research achievements obtained in the field of life science were summarized, focusing on the related applications in recent three years, and some representative research contents in the fields of neurobiology, developmental biology, animal cell biology and plant science were briefly introduced. Finally, the advantages and disadvantages of LSFM were summarized, and its potential applications in optogenetics and multi-group studies were prospected, in order to provide researchers with systematic basic knowledge, latest research applications and potential applications in the future, and provide reference for researchers.
• Research Progress and Application of Liver Organoids
• XIAO Jinping, CAO Yundi, LI Cheng^*, SUN Zhijian
• 2021, 11(2):  148-154.  DOI: 10.19586/j.2095-2341.2020.0126
• Abstract ( ) PDF (1162KB)( )
The susceptibility to liver disease and the liver cell heterogeneity is highly variable, necessitating in vitro personalized hepatic model system towards disease modeling, drug discovery, and drug toxicity studies. There is much advancement in the development of 3D in vitro models in the past decade. They provide a powerful tool for modeling patient-specific disease and establishing personalized therapeutic approaches. Liver organoids represents the original patient-specifific genetic signature and mutations, and maintain liver cells functions in vitro for a long time. They have been used to mimic liver disease, test drug efficacy and toxicity，offering new perspectives in organ transplantation. This review evaluated the progress and the current application of liver organoids from stem cells or primary cells in disease modelling, drug validation and toxicity assessment, which was expected to provide new ideas for research and application of liver organoids in disease modeling, drug validation, and organ transplantation.
• Progress in Anticancer Effect of Disulfiram
• LIU-CHENG Linzi1, WU Yaoqin1, HUANG Enze1, XU Ruifeng1, ZHENG Peng1,2*
• 2021, 11(2):  155-162.  DOI: 10.19586/j.2095-2341.2020.0142
• Abstract ( ) PDF (1184KB)( )
Disulfiram is widely used in clinic as a drug for the treatment of chronic alcoholism. In recent decades, studies have found that it has great potential in the treatment of cancer in addition to its abstinence effect, and research conclusions on its in vitro and in vivo models have been partially confirmed in clinical treatment. Disulfiram inhibits the activity of acetaldehyde dehydrogenase through its metabolites, which leads to the accumulation of acetaldehyde content in vivo, increases cytotoxicity and thus inhibits the proliferation and differentiation of tumor stem cells; increases the concentration of intracellular reactive oxygen species to induce apoptosis; inhibits proteasome activity, accumulates a large number of waste proteins to induce apoptosis; inhibits the down-regulation of NF-kappa B to inhibit epithelial. In addition, the combination of disulfiram and anticancer drugs can improve the efficacy of anticancer drugs. Due to its low toxicity, low cost and tendency to tumor tissues, the reapplication of disulfiram in clinic as an anticancer drug has broad prospects. This article briefly reviewed the molecular mechanism of the anticancer effect of disulfiram as elucidated in the latest studies of disulfiram, and looked forward to the future prospects of disulfiram as a new clinical anticancer drug. The paper was expected to provide reference for the application research of disulfiram in anticancer drug.
• Progress on the Application of Platelet-rich Plasma Injection in Knee Osteoarthritis
• XING Hengte, QIU Bo^*
• 2021, 11(2):  163-169.  DOI: 10.19586/j.2095-2341.2020.0153
• Abstract ( ) PDF (1153KB)( )
Platelet-rich plasma (PRP) has a lot of active growth factors, which can stimulate the proliferation of chondrocytes, promote the proliferation and migration of cartilage precursor cells, induce cartilage precursor cells differentiate into chondrocytes, promote collagen synthesis as well as inhibit the inflammatory response and degeneration of cartilage, provide an internal environment conductive to cartilage repair and delay disease development. In recent years, PRP injection has become a new choice for the treatment of osteoarthritis (OA), and has significant therapeutic effect. To further increase its effectiveness, PRP injection therapy has been performed not only in the articular cavity but also under the subchondral bone. Subchondral bone lesions will accelerate cartilage loss, so it is necessary to consider subchondral bone as one of the key points of the pathogenesis and pathological process of OA. In this paper, the bioeffects of PRP and the application of PRP injection in the treatment of knee osteoarthritis (KOA) were reviewed. Meanwhile, this paper prospected the research of PRP treatment which was injected under the subchondral bone for KOA, to seek more effective methods for the treatments of KOA.
• Progress of Abnormal Glutamate-glutamine Cycle in Autism Spectrum Disorders
• YUAN Qifeng, YAO Baozhen^*
• 2021, 11(2):  170-175.  DOI: 10.19586/j.2095-2341.2020.0110
• Abstract ( ) PDF (1785KB)( )
Autism spectrum disorder (ASD) is a neurodevelopmental disease with multiple causes, which has a high prevalence rate and complicated causes, including inflammation, autoimmunity, genetic abnormality, etc., but the specific pathogenesis is still unclear. Glutamate is the most important excitatory neurotransmitter and a potential neurotoxin in the central nervous system (CNS) of mammals, and its excitotoxicity may lead to the death of nerve cells. However, the metabolic coupling of glutamate and glutamine between astrocytes and neurons prevents excessive glutamate from spreading to peripheral neurons, thus avoiding excessive excitation of neurons and protecting neurons. Studies have shown that abnormal glutamate-glutamine cycle may be the core mechanism of ASD. Therefore, the relationship between the classical causes of autism spectrum disorder, such as inflammation, autoimmunity and genetic abnormality, and the glutamate-glutamine cycle was reviewed, in order to provide a new idea for the classification and treatment of autism spectrum disorder.
• Mapping QTLs Related to Tolerance to Cadmium in Rice During Germination by Backcross Recombinant Inbred Lines
• HUANG Shiying1, TAN Jingai1, WANG Peng1, JIANG Ningfei2, HE Haohua1,2, BIAN Jianmin1,2*
• 2021, 11(2):  176-181.  DOI: 10.19586/j.2095-2341.2020.0135
• Abstract ( ) PDF (2144KB)( )
Rice is one of the most important food crops in the world. At present, there is a problem of heavy metal pollution in agricultural land, but the tolerance of rice to heavy metals such as cadmium (Cd) is low, which affects the yield and quality of rice. Mapping quantitative trait loci (QTLs) related to Cd tolerance in rice is of great significance for guiding the breeding of Cd tolerance in rice. To explore Cd stress related genes, 124 backcross recombinant inbred ines (BILs) derived from Japonica rice 02428 and Indica rice Changhui 891 were used as materials to analyze the root length and bud length of rice at germination stage, and to locate QTLs related to Cd stress at germination period. The results showed that the root length and bud length of 02428 and Changhui 891 were significantly inhibited under Cd stress (P<0.01), and the inhibition of Cd on root length was stronger than that of bud length. Five QTLs related to Cd stress were detected by QTL analysis: qCdBL3, qCdRL7, qCdBL8.1, qCdBL8.2 and qCdBL9, which were located on chromosome 3, 7, 8, 8 and 9 of rice, respectively, with a contribution rate of 6.45%~19.46%. Among them, qCdBL3, qCdBL8.1, qCdBL8.2 and qCdBL9 were related to bud length, and qCdRL7 was related to root length. At the same time, two  QTLs affecting root length and bud length were detected under control conditions (aqueous solution): qCKBL8 and qCKRL4, which were located on chromosome 8 and 4, respectively, with the contribution rates of 10.53% and 1089%. Comparison showed that QTLs controlling root length or bud length of rice in germination stage were different between control and Cd treatment, which indicated that the genetic mechanism controlling root length and bud length of rice under Cd stress might be different from that under non-Cd stress. The results provided a reference for cloning Cd-tolerant genes and breeding new Cd-tolerant rice varieties.
• Identification and Characterization of an Glycotransferase Family Gene SMALL AND EMERALD1 in Medicago truncatula
• SUN Zhikang, WANG Na, MENG Yingying, LIN Hao, NIU Lifang^*
• 2021, 11(2):  182-189.  DOI: 10.19586/j.2095-2341.2021.0006
• Abstract ( ) PDF (7784KB)( )
Glycosyltransferases play an important role in the flavonoid metabolism, which is important for plant development and plant-environment interplay. In order to study the biological function of glycosyltransferase in Medicago truncatula, a series of small and emerald1 (se1) mutants with short plant and dark green leaves by directional screening Tnt1 retrotransposon-tagged lines of M. truncatula was isolated and characterized. The SE1 gene was cloned by PCR-based genotyping of flanking sequence tags (FST) in segregating populations. SE1 encoded a glycosyltransferase that had 52.8% homology with AtUGT84A1, which regulated flavonoid biosynthesis in Arabidopsis. Flavonoid analysis showed that the content of total flavonoids was significantly reduced in the se1 mutants. qRT-PCR analysis revealed that the expression of CHS, F3H and F3’H, key genes of flavonoids synthesis pathway, were down-regulated in the se1 mutants. Subcellular localization showed that SE1 might fulfill its functions in both cytoplasm and nucleus. These data together demonstrated that the glycoltransferase gene SE1 might be involved in the regulation of flavonoid metabolism in M. truncatula, thus influencing M. truncatula growth and development. In addition, the study also revealed that the SE1 gene could negatively regulate M. truncatula chlorophyll biosynthesis.
• Effects of Temperature and Salinity on Growth and Nutrient Absorption of Codium fragile Hariot
• WEI Liyuan^1,2, ZHENG Yi^1*
• 2021, 11(2):  190-195.  DOI: 10.19586/j.2095-2341.2020.0133
• Abstract ( ) PDF (1775KB)( )
With the rapid development of aquaculture, the problem of marine eutrophication is increasing. Macroalgae can effectively control and repair the eutrophic sea area by utilizing its super-absorption ability of nitrogen and phosphorus nutrients. In order to analyze the growth conditions of Codium fragile Hariot in mariculture areas, which has high economic value, taken from the raft in Luoyuan Bay, Lianjiang County, Fujian Province, the effects of temperature (18, 25 and 32 ℃) and salinity (10, 20 and 30) on the growth and nutrient absorption of Codium fragile Hariot were studied under laboratory conditions. The results showed that the growth of Codium fragile Hariot was significantly affected by temperature (P<0.01), salinity and their interaction (P<0.05). Under the conditions of 25 ℃ and salinity 20, the specific growth rate (SGR) of Codium fragile Hariot was the highest, reaching 89.45%. Under the conditions of 32 ℃ and salinity 30, the absorption of nutrients by Codium fragile Hariot was the best, especially for NH+4-N. The research results provided some references for the selection of bioremediation population and the artificial culture of Codium fragile Hariot.
• Diversity Analysis of Sulfamethoxazole Degradation Community and Isolation and Characterization of Degrading Microorganism
• ZHAO Dongxue1§, LIU Lu2§, MU Yingchun2, HAN Gang2, ZHANG Hongyu2, FANG Hongbo3, RUAN Zhiyong4*, SONG Jinlong2*
• 2021, 11(2):  196-203.  DOI: 10.19586/j.2095-2341.2020.0155
• Abstract ( ) PDF (6532KB)( )
In order to solve the pollution caused by sulfamethoxazole in aquatic water, the antibacterial agent sulfamethoxazole was used as the sole carbon source, and the microflora to degrade sulfamethoxazole was obtained by four continuous subculture enrichment. 16S V3-V4 high-throughput sequencing was used to analyze the community composition and the abundance changes in each generation of bacteria, and the strain information related to sulfamethoxazole degradation was speculated. The strain LLE3 to degrade sulfamethoxazole was obtained by further isolation and purification. LLE3 was preliminarily identified as Sphingobacterium by 16S rDNA and physiological and biochemical identification. The degradation conditions of the bacteria were optimized. Under the optimal degradation conditions (30 ℃, pH 7, inoculation concentration of 5×107 CFU·mL-1), the bacteria can degrade 50 mg·L-1 sulfamethoxazole with an efficiency of 93.7% within 7 days. The metabolite degraded by strain LLE3 was identified as N-\[4-(1,2-oxazol-3-yl sulfamoyl) phenyl\] acetamide by high performance liquid chromatography and mass spectrometry. These results provide excellent microbial resources for the remediation of water and soil environment polluted by sulfamethoxazole.
• Study on the Immunotoxicity of Short-term Exposure of Decabromodiphenyl Ether (BDE-209) in Adult Mice
• LIAO Taotao1, TANG Junjie1, SHI Xiaoxiang1, ZHANG Weijie2, MAO Guanghua1*, WU Xiangyang1*
• 2021, 11(2):  204-213.  DOI: 10.19586/j.2095-2341.2020.0140
• Abstract ( ) PDF (3990KB)( )
To investigate the immunotoxicity of short-term exposure to decabromodiphenyl ether (BDE-209) and its self-recovery in adult mice, a model of short-term exposure of BDE-209 in female Balb/c mice was established. Organ index, immunoglobulin, lymphocyte proliferation, lipid peroxidation, immune cytokine and genes were investigated on 1 d and 21 d after the end of administration, respectively. And integrated evaluation was conducted by integrated biomarker responses combined with principal component analysis and factorial analysis. The results showed that BDE-209 could reduce the index of immune organs, accompanied by oxidative damage. BDE-209 could stimulate the secretion of IgG and inhibit the proliferation of spleen lymphocytes. BDE-209 could change the secretion of cytokines and gene expression related to Th1 and Th2 cells, causing Th1/Th2 imbalance. The comprehensive analysis showed that the immunotoxicity of BDE-209 was reduced 21 days after the end of exposure, and there was an interaction between the exposure dose of BDE-209 and whether the recovery period was passed. The results showed that short term exposure to BDE-209 has immunotoxicity in adult mice, but this effect could be reduced to some extent by self-recovery.
• Optimization of Hydrolysis Conditions of N-glycan from Monoclonal Antibodies by PNGase F
• ZHANG Bohui, JIA Daihui, CHENG Qian, XU Junyan^*, SHAO Zhe, HUANG Yingfeng
• 2021, 11(2):  214-222.  DOI: 10.19586/j.2095-2341.2021.0005
• Abstract ( ) PDF (9089KB)( )
In order to  develop and optimize a method of hydrolyzing N-glycan from monoclonal antibodies by using PNGase F, a series of experimental conditions, including buffer pH, enzyme type, instruments, degree of enzymatic hydrolysis, denaturing buffer solution and enzyme dosage were optimized by using several monoclonal antibodies. Results showed that, buffer exchanging into 1×PBS could avoid the conversion of some mAbs from G0F to G0F-GN at low pH and improve the peak shape. Rapid PNGase F and denature buffer could effectively improve the efficiency of enzymatic hydrolysis. Combination of UPLC and chromatographic column with 1.7 μm particle size could improve the resolution. Incomplete enzymatic hydrolysis could affect the results of N-glycan content. Results indicated that the optimized hydrolyzing conditions of PNGase F can quickly and effectively hydrolyze the N-glycan from the monoclonal antibody, which can provide an effective method for controlling N-glycan content in cell line screening and quality control of monoclonal antibody drugs.
• #br# Analysis of Genetic Polymorphisms at 38 Y-STR Loci in Tongchuan Han Population
• JIN Tao1, TIAN Yang1, QUAN Yanzhao1, LI Mingliang1, YAN Anxin2, LI Luyao3*
• 2021, 11(2):  223-230.  DOI: 10.19586/j.2095-2341.2020.0164
• Abstract ( ) PDF (8072KB)( )
Y chromosome short tandem repeats （Y-STRs） can serve as a promising and useful supporting tool in forensic research, especially in the analysis of male components of mixed stains and pedigree examination. With the development of Y-STR commercial kits and the establishment of databases, numerous studies on Y-STR polymorphism in different regions and populations is increasing. However, there are few reports on the Y-STR related study of Tongchuan Han population. Based on this, the genetic polymorphisms of 38 Y-STR loci in 669 unrelated healthy male individuals in Tongchuan Han population in Shaanxi province was investigated by  YfilerTM Platinum Multiplex Amplification Kit, and the genetic relationship between Tongchuan Han nationality and other populations was explored. A total of 657 unique haplotypes were observed in Tongchuan Han population. Haploid diversity and discrimination power were 0.999 937 345 and 0.982 1, respectively. 428 alleles were detected in 38 Y-STR loci, with the gene diversities ranging from 0.108 9 (DYS645) to 0.969 9 (DYS385). Genetic distance, multi-dimensional scaling (MDS) and phylogenetic tree were consistent that the population relationship between Tongchuan Han population and other Han groups from different geographical regions was close. In conclusion, the forensic genetic parameters obtained based on 38 Y-STR loci had a high genetic polymorphism in Tongchuan Han population, which had potential application value in inferring male suspects in forensic crime scene in Tongchuan.
• Strategy for the Soluble Expression of the Astragalus membranaceus Pathogenesis-related Protein (AmPR-10) with Different Vectors of E. coli
• WANG Jue, HU Lili, LI Guilan, WU Na, ZHANG Yumin^*
• 2021, 11(2):  231-237.  DOI: 10.19586/j.2095-2341.2020.0158
• Abstract ( ) PDF (6273KB)( )
Astraglus membranaceus pathogenesis-related protein-10 (AmPR-10) has nuclease activity, which is of great significance to the growth and development of Astragalus membranaceus and its disease resistance mechanism. However, the traditional method of extracting protein from natural Astragalus membranaceus has higher cost and less protein yield. For the first time, Escherichia coli expression system was used to express AmPR-10, and three kinds of recombinants were constructed: ① pET28a-AmPR-10 constructed by pET28a; ② pET30a-AmPR-10 constructed by pET30a; ③ pET30a-skp-AmPR-10 constructed with the E. coli chaperone skp by pET30a. According to SDS-PAGE analysis, the soluble expression of target protein was pET30a-skp-AmPR-10 > pET30a-AmPR-10 > pET28a-AmPR-10. According to the simulation analysis of protein tertiary structure, the tag S-tag on the vector pET-30a could improve the soluble expression of the target protein by influencing the local α helix conformation of AmPR-10, and the molecular chaperone skp could further improve the soluble expression of the target protein by increasing the overall α helix ratio of the fusion protein. The study solved the problems of complex operation and small amount of protein extraction from natural Astragalus membranaceus. At the same time, the target protein was detected to be with nuclease activity, which provide a basis for the study of related activities of exogenous AmPR-10.
• The Regulation Effect of RTN4 on Colon Cancer Cell Proliferation
• YANG Mengtian¹, YUAN Jumao^2*
• 2021, 11(2):  238-243.  DOI: 10.19586/j.2095-2341.2020.0097
• Abstract ( ) PDF (4877KB)( )
According to the 2018 Global Cancer Statistics Survey, colon cancer account for 12.1% of new cancer cases. Therefore, it is urgent to find new genes related to the occurrence of colon cancer and new therapeutic targets. By analyzing the database, we found that the expression levels of RTN4 was significantly correlated with the survival rate of colon cancer patients. In this study, the interfering plasmid of RTN4 gene was constructed, and lentivirus was used as the vector to transfect colon cancer HCT116 cells to construct colon cancer cell lines with RTN4 knock-down. The results showed that RTN4 knock-down significantly promoted the proliferation of colon cancer cell HCT116. Western blot was used to observe the expression of autophagy pathway related proteins p62 and LC3 in HCT116 cells after RTN4 knock-down. It was found that the LC3 transformation amount (LC3-II/LC3-I) was increased and the p62 protein was decreased in the RTN4 knockdown group compared with the control group. In this study, the potential anticancer effects of RTN4 was analyzed. Knockdown of RTN4 gene significantly enhanced the proliferation of colon cancer cells, promoted the proliferation of colon cancer cells, and induced autophagy, which may be related to the activation of LC3/p62 autophagy pathway.
• In vitro Study of the Inhibition Growth of Cervical Cancer Cells by Triptolide-loading TPGS-b-(PCL-ran-PGA) Nanoparticles
• ZHENG Yi1, ZHENG Jingwei2, ZHOU Yuling2, LIANG Xin1, BAO Junli1, YANG Juhong1, ZENG Fanqi1*
• 2021, 11(2):  244-251.  DOI: 10.19586/j.2095-2341.2020.0121
• Abstract ( ) PDF (6458KB)( )
Triptolide(TPL), as a main compound isolated from traditional Chinese medicine Tripterygium wilfordii, has a broad range of bioactivities including anti inflammation and anticancer activity. However, its clinical application of TPL is restricted by its adverse effects. To explore the characteristics of TPGS-b-(PCL-ran-PGA)/TPL nanoparticles（NPs） and functions as anti-cervical cancer cells in vitro, TPGS-b-(PCL-ran-PGA)/TPL NPs were prepared by using emulsion/solvent evaporation method and optimizing the ratio of TPL and TPGS-b-(PCL-ran-PGA), and their size distribution, morphology, zeta potential, drug encapsulation efficiency, drug-release profile, were characterized. The 50% inhibitory concentration(IC50) and inhibition effect of both free TPL and TPGS-b-(PCL-ran-PGA)/TPL NPs on cervical cancer cells were measured by MTS method and colony formation assay, respectively. By using flow cytometer, HeLa cell apoptosis induced by TPGS-b-(PCL-ran-PGA)/TPL NPs was detected. Results showed that, TPGS-b-(PCL-ran-PGA)/TPL NPs were prepared at the 50∶1 ratio of TPGS-b-(PCL-ran-PGA)and TPL. The size of TPGS-b-(PCL-ran-PGA)/TPL NPs was (95.3±5.2) nm with a mean zeta potential (-12.2±0.9) mV.  A bi-phase drug-release profile from these NPs was observed at pH 7.4 in drug release studies. TPGS-b-(PCL-ran-PGA)/TPL NPs（IC50 2.8,1.8, 0.9 μg·L-1 at 24, 48 and 72 h, respectively）were proved more potent against HeLa cells than free TPL (IC50  232,12.5 and 6.8 at 24, 48 and 72 h, respectively). TPGS-b-(PCL-ran-PGA)/TPL NPs could significantly inhibit tumor cell colony formation and induce apoptosis compared with free TPL. The results indicated that, TPGS-b-(PCL-ran-PGA)/TPL NPs could inhibit the cancer cell growth in vitro, since in combination of TPL and TPGS leads to apoptosis. It suggested that TPGS-b-(PCL-ran-PGA)/TPLNPs can serve as candidate agents against cancers including cervical cancer.