Curr. Biotech. ›› 2014, Vol. 4 ›› Issue (1): 30-35.DOI: 10.3969/j.issn.2095-2341.2014.01.06

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Effects of Nitric Oxide Treatment on Polygalacturonase Activity and Expression of MaPGs Genes in Harvested Banana Fruit During Storage

WU Bin, GUO Qin, WANG Gang-xia, CHEN Wei-xin, LI Xue-ping   

  1. 1.Guangdong Province Key Laboratory for Postharvest Technology of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, China;
    2.Institute of Agro\|products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
    3.Preservation and Logistics Division, Institute of Agro\|products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2013-10-14 Online:2014-01-25 Published:2013-11-08

Abstract: In order to explore the molecular regulation mechanism of polygalacturonase (PG) during banana softening, fruit harvested at the mature green stage were fumigated with 40 μL/L of NO for 3 h, and then stored at 20℃ with 85% relative humility. Ethylene production, firmness, PG activity and MaPGs genes were measured during banana ripening. The results showed that NO treatment resulted in significantly lower rates of ethylene production than those in the control fruit. The NO treated fruit underwent a lesser loss of firmness during storage. The activity of PG and expression of MaPG2、MaPG3 and MaPG4 genes were effectively reduced in NO treated fruit, indicating that the softening of banana fruit was delayed by NO treatment.

Key words: nitric oxide, banana, softening, polygalacturonase, gene