Current Status and Hotspot Analysis on Tobacco Biotechnology Research Based on Bibliometrics

doi:10.19586/j.2095-2341.2026.0013

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (4): 941-951.DOI: 10.19586/j.2095-2341.2026.0013

• Articles • Previous Articles Next Articles

Current Status and Hotspot Analysis on Tobacco Biotechnology Research Based on Bibliometrics

Pan WEI¹(), Peijian CAO¹^,², Qiansi CHEN¹()

^1.Zhengzhou Tobacco Research Institute，China National Tobacco Corporation，Zhengzhou 450001，China
^2.Beijing Life Science Academy，Beijing 102200，China

Received:2026-01-09 Accepted:2026-03-31 Online:2026-07-25 Published:2026-09-11
Contact: Qiansi CHEN

Abstract

Abstract:

Using the Web of Science （WOS） database and the VOSviewer literature analysis tool， the relevant literature in the field of tobacco biotechnology research from 2005 to 2024 was analyzed to explore the research status and hot spots of tobacco biotechnology. The results showed that the number of tobacco biotechnology research papers published in the United States and China in the past two decades ranked the top two in the world. Especially since 2020， the number of tobacco biotechnology research papers published in China has become the first in the world. The United States has the most frequent cooperation in tobacco biotechnology research with countries around the world， and China temporarily ranks third. The research strength of tobacco biotechnology in China has been greatly improved， showing a good development trend and gradually narrowing the gap with developed countries in Europe and the United States. However， more efforts are still needed in international exchange and cooperation and high-quality research. Biological breeding， functional gene research， microbial resource development and utilization， biosynthesis and other fields are the hotspots of tobacco biotechnology research in recent years. It is necessary to further increase investment in basic scientific research and pay attention to the transformation of scientific research results.

Key words: tobacco, biotechnology, bibliometrics, analysis of research hotspot

CLC Number:

Pan WEI, Peijian CAO, Qiansi CHEN. Current Status and Hotspot Analysis on Tobacco Biotechnology Research Based on Bibliometrics[J]. Current Biotechnology, 2026, 16(4): 941-951.

Add to citation manager EndNote|Ris|BibTeX

URL: https://swjsjz.magtechjournal.com/EN/10.19586/j.2095-2341.2026.0013

https://swjsjz.magtechjournal.com/EN/Y2026/V16/I4/941

Figures/Tables 10

References 35

[1]	DECKER E L, RESKI R. Mosses in biotechnology[J]. Curr. Opin. Biotechnol., 2020, 61: 21-27.
[2]	HUANG W E, YANG C, WANG H, et al.. Microbiology biotechnology in China[J/OL]. Microb. Biotechnol., 2021, 14(2): 322[2025-12-5]. .
[3]	WANG P A, ZHONG J J. Strain breeding and gene complementation strategies for filamentous fungi[J]. Process. Biochem., 2023, 128: 181-189.
[4]	中华人民共和国中央人民政府.中华人民共和国国民经济和社会发展第十四个五年规划和2035年远景目标纲要[EB/OL].(2021-03-13)[2023-04-03]..
[5]	国家发展改革委.关于印发《“十四五”生物经济发展规划》的通知[EB/OL].(2022-05-10)[2023-04-03]..
[6]	LOUKANINA N, STASOLLA C, BELMONTE M F, et al.. Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis[J]. Plant Physiol. Biochem., 2008, 46(7): 665-672.
[7]	ZAMBRYSKI P. Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells[J]. Annu. Rev. Genet., 22(1): 1-30.
[8]	BALLY J, JUNG H, MORTIMER C, et al.. The rise and rise ofNicotiana benthamiana: a plant for all reasons[J]. Annu. Rev. Phytopathol., 2018, 56: 405-426.
[9]	BHATTACHARJEE S, STAHELIN R V, SPEICHER K D, et al.. Endoplasmic reticulum PI(3)P lipid binding targets malaria proteins to the host cell[J]. Cell, 2012, 148(1-2): 201-212.
[10]	ZHAO Y, XU Y, WANG Z, et al.. Genome-wide identification and characterization of an amino acid permease gene family in Nicotiana tabacum [J]. RSC Adv., 2017, 7(60): 38081-38090.
[11]	IIDA A, YAMASHITA T, YAMADA Y, et al.. Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco[J]. Plant Physiol., 1991, 97(4): 1585-1587.
[12]	WANG Y, FAN J, AHMAD N, et al.. Successful production of human epidermal growth factor in tobacco chloroplasts in a biologically active conformation[J]. Growth Factors, 2023, 41(1): 20-31.
[13]	GAO L, CAO X Z, ZHANG Y, et al. Bibliometric analysis of literature regarding ostomy research based on the web of science database[J]. Front. Nursing, 2018, 5(3): 193-198.
[14]	HUANG Y, XU C, ZHANG X, et al.. Bibliometric analysis of landslide research based on the WOS database[J]. Nat. Hazards Res., 2022, 2(2): 49-61.
[15]	白京羽,林晓锋,尹政清.基于文献计量的全球生物技术研究现状与发展趋势分析[J].中国生物工程杂志,2020,40(7):100-109.
	BAI J Y, LIN X F, YIN Z Q. Analysis of the current situation and development trend of global biotechnology research based on bibliometrics[J]. China Biotechnol., 2020, 40(7): 100-109.
[16]	MENG L, WEN K H, BREWIN R, et al.. Knowledge atlas on the relationship between urban street space and residents' health: a bibliometric analysis based on VOSviewer and CiteSpace[J/OL]. Sustainability, 2020, 12(6): 2384[2025-12-25]. .
[17]	TAMALA J K, MARAMAG E I, SIMEON K A, et al.. A bibliometric analysis of sustainable oil and gas production research using VOSviewer[J/OL]. Clean. Eng. Technol., 2022, 7: 100437[2025-12-25]. .
[18]	李锋,王燃,武明珠,等.基于过表达技术的烟草NtGGPPS1基因功能的研究[J].河南大学学报(自然科学版),2019,49(2):180-185.
	LI F, WANG R, WU M Z, et al.. Study on NtGGPPS1 function in Nicotiana tabacum based on overexpression technology[J]. J. Henan Univ. Nat. Sci., 2019, 49(2): 180-185.
[19]	胡贲,刘燕翔,王燃,等.本氏烟β-1,3-半乳糖苷转移酶基因鉴定、进化及表达分析[J].河南大学学报(自然科学版),2020,50(2):201-207.
	HU B, LIU Y X, WANG R, et al.. Identification, evolution and expression analysis of beta-1, 3-galactosyltransferase genes in Nicotiana benthamiana [J]. J. Henan Univ. Nat. Sci., 2020, 50(2): 201-207.
[20]	王燃,史艳梅,郑凯,等.烟草玉米黄质环氧化酶基因的转录表达模式及其功能研究[J].河南大学学报(自然科学版),2017,47(1):55-61+85.
	WANG R, SHI Y M, ZHENG K, et al.. The transcriptional expression and functional analysis of zeaxanthin epoxidase gene in Nicotiana tabacum [J]. J. Henan Univ. Nat. Sci., 2017, 47(1): 55-61+85.
[21]	LI B, SHANG Y, WANG L, et al.. Efficient genome editing in dicot plants using calreticulin promoter-driven CRISPR/Cassystem[J/OL]. Mol. Hortic., 2025, 5(1): 9[2025-12-25]. .
[22]	FANG N, DU Z, LIU X, et al.. Engineering tobacco for efficient astaxanthin production using a linker-free monocistronic dual-protein expression system and interspecific hybridization method[J/OL]. Plant Physiol. Biochem., 2025, 221: 109607[2025-12-25]. .
[23]	LI Y, HUANG C, LIU Y, et al.. CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum [J]. Plant Biotechnol. J., 2023, 21(12): 2641-2653.
[24]	MA X, ZHANG X, LIU H, et al.. Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9[J]. Nat. Plants, 2020, 6(7): 773-779.
[25]	XU Q, TANG C, WANG X, et al.. An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function[J/OL]. Nat. Commun., 2019, 10: 5571[2025-12-25]. .
[26]	FORNER J, KLEINSCHMIDT D, MEYER E H, et al.. Targeted introduction of heritable point mutations into the plant mitochondrial genome[J]. Nat. Plants, 2022, 8(3): 245-256.
[27]	QIAO Z, KONG Q, TEE W T, et al.. Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis[J/OL]. Sci. Adv., 2022, 8(34): eabq1211[2025-12-25]. .
[28]	WANG Y, XU Y, SUN Y, et al.. Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection[J/OL]. Nat. Commun., 2018, 9: 594[2025-12-25]. .
[29]	SIERRO N, BATTEY J N D, OUADI S, et al.. The tobacco genome sequence and its comparison with those of tomato and potato[J/OL]. Nat. Commun., 2014, 5: 3833[2025-12-25]. .
[30]	LI J, HALITSCHKE R, LI D, et al.. Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity[J]. Science, 2021, 371(6526): 255-260.
[31]	MICHARD E, LIMA P T, BORGES F, et al.. Glutamate receptor-like genes form Ca²⁺ channels in pollen tubes and are regulated by pistild-serine[J]. Science, 2011, 332(6028): 434-437.
[32]	YONG L, JIE W, CHONGKUN Z, et al.. Identification of a gene conferring broad-spectrum Orthotospovirus resistance in Solanaceae[J]. Sci. Adv., 2025, 11(25): eadw4333[2025-12-25]. .
[33]	LIU Z, HOU L, YAN J, et al.. Aquaporin mediated silicon-enhanced root hydraulic conductance is benefit to cadmium dilution in tobacco seedlings[J/OL]. J. Hazard. Mater., 2024, 476: 134905[2025-12-25]. .
[34]	YANG Z, LIU T, FAN J, et al.. Biocontrol agents modulate phyllosphere microbiota interactions against pathogen Pseudomonas syringae [J/OL]. Environ. Sci. Ecotechnol., 2024, 21: 100431[2025-12-25]. .
[35]	SONG S J, DIAO H P, ZHANG S S, et al.. Natural peptidoglycan nanoparticles enable rapid antigen purification and potent delivery of plant-derived vaccines[J/OL]. Plant Commun., 2025, 6(8): 101418[2025-12-25]. .

分析类型	分析单位	计数方法	阈值设置	特殊处理
关键词共现	All keywords	Full counting	出现频次≥5次	同义词合并，剔除宽泛词汇
国家合作	Countries	Full counting	发文量≥5篇	-
时间演进	关键词	-	切片跨度4年	颜色映射平均年份

分析类型	分析单位	计数方法	阈值设置	特殊处理
关键词共现	All keywords	Full counting	出现频次≥5次	同义词合并，剔除宽泛词汇
国家合作	Countries	Full counting	发文量≥5篇	-
时间演进	关键词	-	切片跨度4年	颜色映射平均年份

阶段	特征	演进意义
第一阶段（2005—2012）：基础技术奠基期	高频关键词集中于农杆菌（Agrobacterium）、转基因植物（transgenic plants）、愈伤组织（callus）、抗菌（antibacterial），这表明研究核心是遗传转化体系的优化和抗性性状的初步导入	为烟草作为生物技术底盘奠定了成熟的遗传操作基础
第二阶段（2013—2018）：分子机制解析与多用途探索期	基因表达（gene expression）、代谢工程（metabolic engineering）、重组蛋白（recombinant protein）、疫苗（vaccine）等关键词突现，研究焦点从“能否转化”转向“如何精确调控”和“用于生产什么”	标志着烟草从模式植物向“生物反应器”和“合成生物学底盘”概念拓展，基础研究与应用开发开始结合
第三阶段（2019—2024）：前沿交叉与精准智能化期	CRISPR/Cas9、合成生物学（synthetic biology）、纳米颗粒（nanoparticles）、微生物组（microbiome）、机器学习（machine learning）成为最强突显词，研究呈现工具革命性（基因编辑）、系统集成性（合成生物学）、尺度交叉性（纳米-生物界面）和方法智能化特征	烟草生物技术深度融入生命科学前沿，致力于解决精准育种、绿色生物制造、智能农业等更复杂挑战

阶段	特征	演进意义
第一阶段（2005—2012）：基础技术奠基期	高频关键词集中于农杆菌（Agrobacterium）、转基因植物（transgenic plants）、愈伤组织（callus）、抗菌（antibacterial），这表明研究核心是遗传转化体系的优化和抗性性状的初步导入	为烟草作为生物技术底盘奠定了成熟的遗传操作基础
第二阶段（2013—2018）：分子机制解析与多用途探索期	基因表达（gene expression）、代谢工程（metabolic engineering）、重组蛋白（recombinant protein）、疫苗（vaccine）等关键词突现，研究焦点从“能否转化”转向“如何精确调控”和“用于生产什么”	标志着烟草从模式植物向“生物反应器”和“合成生物学底盘”概念拓展，基础研究与应用开发开始结合
第三阶段（2019—2024）：前沿交叉与精准智能化期	CRISPR/Cas9、合成生物学（synthetic biology）、纳米颗粒（nanoparticles）、微生物组（microbiome）、机器学习（machine learning）成为最强突显词，研究呈现工具革命性（基因编辑）、系统集成性（合成生物学）、尺度交叉性（纳米-生物界面）和方法智能化特征	烟草生物技术深度融入生命科学前沿，致力于解决精准育种、绿色生物制造、智能农业等更复杂挑战

排名	国家	高被引论文数/篇	占全球比例/%	三大顶刊系列论文数/篇
1	美国	18	62.1	32
2	中国	6	20.7	11
3	德国	3	10.3	5
4	英国	2	6.9	4

Current Status and Hotspot Analysis on Tobacco Biotechnology Research Based on Bibliometrics

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 35

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Yu GAO, Tianyi HAN, Xiaoyun HE, Nan CHENG, Weichen LIU, Wei FU, Kunlun HUANG, Hong CHEN. Research Progress on Multi-omics Systematic Analysis of Unintended Effects in Biotechnology-derived Breeding Products [J]. Current Biotechnology, 2026, 16(4): 732-743.
[2]	Tianyi HAN, Yu GAO, Nan CHENG, Xiaoyun HE, Weichen LIU, Wei FU, Kunlun HUANG, Hong CHEN. Innovative Applications of Artificial Intelligence in Safety Assessment of Biological Breeding Products [J]. Current Biotechnology, 2026, 16(4): 765-773.
[3]	Xiaochun REN, Xin LIU, Ying HAN, Kai DUAN, Feng LIU, Ruili HAO, Huan SI, Yingqun HUANG, Jianjun XIA. Effects of Nitrogen Application on Yield-quality Formation and Aroma Compounds Accumulation in Flue-cured Tobacco ‘Yunyan 87’ [J]. Current Biotechnology, 2026, 16(1): 76-85.
[4]	Qiang JI, Na WU, Chao ZHENG, Zhikang SUN, Han WU, Xuanwen LI, Jie HAO. Recent Advances in Biosynthesis and Application of Chlorogenic Acid from Tobacco [J]. Current Biotechnology, 2025, 15(6): 933-943.
[5]	Xiaomin LIU, Ting LU, Yong LI, Meng WANG, Baokun ZHU, Wei ZHANG. The Impact of Enzyme Treatment on Yeast Fermentation of Tobacco [J]. Current Biotechnology, 2025, 15(1): 93-101.
[6]	Sujie WANG, Mengli GU, Jiemeng TAO, Zhijun TONG, Junjia GUO, Jingjing JIN, Mengxiao XU, Lijun MENG, Jianfeng ZHANG, Peijian CAO, Peng LU. Isolation, Identification and Whole-genome Sequence Analysis of Phytophthora nicotianae Antagonistic Bacteria XC-29 [J]. Current Biotechnology, 2024, 14(6): 1004-1015.
[7]	Lin XIA, Xiangli XU, Xueyun WANG, Jun YANG, Mingzhu WU, Weiwu SONG. Research Progress on the Biosynthesis of Chlorogenic Acid in Plant [J]. Current Biotechnology, 2024, 14(6): 973-979.
[8]	Bowen WANG, Jinxing WANG, Chengyao QIU, Qingxian QIN, Juxiang WANG, Bo YAO, Hongyan LI, Guanxin TAN, Yingwei YANG, Guanghai JI. Control of Tobacco Bacterial Wilt by Bacillus velezensis Combined with Calcium-containing Soil Conditioner [J]. Current Biotechnology, 2024, 14(5): 839-847.
[9]	Mengmeng KONG, Peng LU, Qiansi CHEN, Xueyi QIAO, Shanyi CHEN, Jingjing JIN, Xueao ZHENG, Peijian CAO, Jiemeng TAO. Construction of Cellulase Producing Engineering Strains and the Application in Aged Tobacco Leaves [J]. Current Biotechnology, 2024, 14(2): 263-270.
[10]	Zilin SAI, Hongling QI, Yun LI, Yu WANG, Yichao GENG. Effects of Biochar Addition on Soil Nutrients, Yield and Quality of Flue-cured Tobacco [J]. Current Biotechnology, 2024, 14(2): 271-277.
[11]	Kehao CAO, Junli ZHU, Huashan HE, Weizhuo XU. Impact of the Fourth Modifications of Patent Laws on Biotechnology Patent Applications and Industry Development [J]. Current Biotechnology, 2023, 13(5): 663-670.
[12]	Jie HAO, Xuanwen LI, Bao ZHANG, Chao ZHENG, Zhikang SUN, Qiang JI, Na WU, Han WU, Liqun LI. Application Progress of Cellulase in Tobacco [J]. Current Biotechnology, 2023, 13(2): 166-173.
[13]	Doudou LEI, Runran MA, Jiabo WANG, Weijun KONG. Research Progress on New Biological Detection Technology for Pesticide Residues in Foods [J]. Current Biotechnology, 2023, 13(1): 1-10.
[14]	Jie HAO, Qiang JI, Liqun LI, Chao ZHENG, Na WU, Han WU, Xuanwen LI, Zhikang SUN. Research Progress on Improving Aroma of Tobacco Leaf with Bio-enzymes and Microorganism Technology [J]. Current Biotechnology, 2022, 12(6): 817-824.
[15]	Jinbang WANG, Jiqing QIU, Zhibo WANG, Ling LI, Zhijing LU, Jun ZOU, Yan CHEN. Research Progress of Tobacco Wastes in the Field of Compost [J]. Current Biotechnology, 2022, 12(5): 711-720.