Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro

doi:10.19586/j.2095-2341.2023.0105

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (4): 934-940.DOI: 10.19586/j.2095-2341.2023.0105

• Articles • Previous Articles Next Articles

Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro

Ming LIU¹^,²(), Junxin ZHANG¹^,², Zhimin HE¹^,², Liping YU¹^,², Tiran HUANG¹^,²(), Lanqing MA¹^,²^,³(), Liusheng DUAN⁴

^1.Bioscience and Resources Environment，Beijing University of Agriculture College，Beijing 102206，China
^2.Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs，Beijing 102206，China
^3.Department of Criminal Science and Technology，Beijing Police College，Beijing 102202，China
^4.College of Plant Science and Technology，Beijing University of Agriculture，Beijing 102206，China

Received:2023-09-11 Accepted:2023-11-08 Online:2026-07-25 Published:2026-09-11
Contact: Tiran HUANG,Lanqing MA

Abstract

Abstract:

To rapid propagate high-quality Reynoutria japonica Houtt. seedlings and optimize a rapid propagation system of Reynoutria japonica Houtt. in vitro tissue， the effects of different disinfection times on the stem segments of Reynoutria japonica Houtt. and different hormone concentrations in the culture medium on the proliferation induction and adventitious bud rooting of the stem segments of Reynoutria japonica Houtt. were investigated. The results showed that the best sterilization method for the stem segment with axillary buds of Reynoutria japonica Houtt. was 75% alcohol 15 s+（0.1% Tween-20+2% sodium hypochlorite） for 12 min， and the acquisition rate of sterile explants reached 90.89%. The optimal medium for inducing axillary bud sprouting in stem segments was MS+30 g·L^-1 sucrose+7 g·L^-1 agar+1.5 mg·L^-1 kinetin+0.2 mg·L^-1 naphthalene acetic acid， with a proliferation rate of 113.3%. The optimal medium for rooting culture of adventitious buds was 1/2 MS+30 g·L^-1 sucrose+5.5 g·L^-1 agar+3 mg·L^-1 green plant growth regulator （GGR6）. The rooting rate could reach 70.37% and adventitious roots could be differentiated after about 3 days， and complete plants could be obtained after about 15 days. The survival rate of transplanted tissue cultured seedlings after rooting reached 100%. This study optimized a rapid culture system for stem segments of Reynoutria japonica Houtt.， with a sterile explant acquisition rate， proliferation rate， and transplant survival rate of over 90%. Moreover， the rooting time is fast， which can significantly shorten the transplant time and reduce reproduction costs.

Key words: Reynoutria japonica Houtt., rapid propagation in vitro, green plant growth regulator

CLC Number:

Q945.5

Ming LIU, Junxin ZHANG, Zhimin HE, Liping YU, Tiran HUANG, Lanqing MA, Liusheng DUAN. Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro[J]. Current Biotechnology, 2026, 16(4): 934-940.

Add to citation manager EndNote|Ris|BibTeX

URL: https://swjsjz.magtechjournal.com/EN/10.19586/j.2095-2341.2023.0105

https://swjsjz.magtechjournal.com/EN/Y2026/V16/I4/934

Figures/Tables 6

References 21

[1]	陈奇.中药药理研究方法学[M].第2版.北京:人民卫生出版社,2006.
	CHEN Q. Methodology of pharmacological research on traditional Chinese medicine[M]. 2nd ed. BeiJing: People's Health Publishing House, 2006.
[2]	孔晓华,周玲芝.中药虎杖的研究进展[J].中医药导报,2009,15(5):107-110.
	KONG X H, ZHOU L Z. Study progress of giant knotweed rhizome[J]. Guid. J. Tradit. Chin. Med. Pharm., 2009, 15(5): 107-110.
[3]	时圣明,潘明佳,王文倩,等.虎杖的化学成分及药理作用研究进展[J].药物评价研究,2016,39(2):317-321.
	SHI S M, PAN M J, WANG W Q, et al.. Research progress on chemical constituents in roots and rhizomes of Polygonum cuspidatum and their pharmacological activities[J]. Drug Eval. Res., 2016, 39(2): 317-321.
[4]	李聪.虎杖的药用价值及种植前景分析[J].乡村科技,2014(17):11.
	LI C. Analysis of medicinal value and planting prospect of Polygonum cuspidatum [J]. Rural Technol., 2014(17): 11.
[5]	陈建业,程炳嵩.虎杖的茎段培养[J].植物生理学通讯,1988,24(6):41.
	CHEN J Y, CHENG B S. Tissue culture of explants from Polygonum cuspidatum [J]. Plant Physiol. Commun., 1988, 24(6): 41.
[6]	杨培君,李会宁,赵桦.虎杖的组织培养与快速繁殖[J].西北植物学报,2003,23(12):2192-2195.
	YANG P J, LI H N, ZHAO H. Tissue culture and rapid propagation of Reynoutria japonica [J]. Acta Bot. Boreali Occidentalia Sin., 2003, 23(12): 2192-2195.
[7]	于树宏,张嫡群,杨双革.TDZ和CPPU在虎杖组织培养中的应用[J].应用与环境生物学报,2006,12(1):13-17.
	YU S H, ZHANG D Q, YANG S G. Application of TDZ and CPPU to tissue culture of Polygonum cuspidatum [J]. Chin. J. Appl. Environ. Biol., 2006, 12(1): 13-17.
[8]	易霭琴,童方平,宋庆安,等.虎杖组织培养技术研究[J].湖南林业科技,2007,34(1):10-12.
	YI A Q, TONG F P, SONG Q A, et al.. The tissue culture technology of Polygonum cuspidatum sieb et zucc[J]. Hunan For. Sci. Technol., 2007, 34(1): 10-12.
[9]	夏海武.虎杖组培快繁影响因素的研究[C]//现代农业理论与实践—安徽现代农业博士科技论坛论文集.合肥,2007:165-167.
[10]	詹立平,赵鑫,黄竹青,等.虎帐组织培养研究[J].辽宁中医药大学学报,2010,12(7):185-186.
	ZHAN L P, ZHAO X, HUANG Z Q, et al.. Study on tissue culture of Polygonum cuspidatum sieb et zucc[J]. J. Liaoning Univ. Tradit. Chin. Med., 2010, 12(7): 185-186.
[11]	洪汉君,熊小灿.一种虎杖组织培养快速繁殖方法:CN104255502A[P].2015-01-07.
[12]	王宇,方晨,刘洋,等.虎杖的组织培养及高效无性系的建立研究[J].江西农业学报,2009,21(3):73-76.
	WANG Y, FANG C, LIU Y, et al.. Tissue culture and establishment of high-efficient clone of Polygonum cuspidatum [J]. Acta Agric. Jiangxi, 2009, 21(3): 73-76.
[13]	杜敏华,杨建伟,杨柯金.虎杖茎尖离体再生体系的建立和优化[J].核农学报,2008,22(5):600-606.
	DU M H, YANG J W, YANG K J. Establishment of optimal regeneration system in vitro for stem apexes of polygonum cuspidatum [J]. J. Nucl. Agric. Sci., 2008, 22(5): 600-606.
[14]	闻永慧,陈双秀,汪琼,等.红纹凤仙花离体快繁技术体系的建立[J].北方园艺,2021(15):57-64.
	WEN Y H, CHEN S X, WANG Q, et al.. Establishment of rapid propagation technology for Impatiens rubrostriata in vitro [J]. North. Hortic., 2021(15): 57-64.
[15]	陈志红,李一澍,卢国杰.知母总皂苷提取分离方法研究[J].应用化工,2008,37(8):841-843.
	CHEN Z H, LI Y S, LU G J. Study on the extraction and separation of timosaponin[J]. Appl. Chem. Ind., 2008, 37(8): 841-843.
[16]	BOUZA L, JACQUES M, MIGINIAC E. In vitro propagation of Paeonia suffruticosa Andr. cv. 'Mme de Vatry': developmental effects of exogenous hormones during the multiplication phase[J]. Sci. Hortic., 1994, 57(3): 241-251.
[17]	祝红艺,柴岩,刘小凤,等.KT与NAA对马铃薯组培苗生长的影响[J].河北农业科学,2000,4(2):7-9.
	ZHU H Y, CHAI Y, LIU X F, et al.. Effect of KT and NAA on potato seedlings growth in tissue culture[J]. J. Hebei Agric. Sci., 2000, 4(2): 7-9.
[18]	PALMER C E. Enhanced shoot regeneration from Brassica campestris by silver nitrate[J]. Plant Cell Rep., 1992, 11(11): 541-545.
[19]	张松,魏毓棠,温孚江,等.利用乙烯抑制剂AgNO₃建立大白菜高频植株再生体系[J].园艺学报,1997,24(1):94-96.
	ZHANG S, WEI Y T, WEN F J, et al.. Establishment of efficient plant regeneration systam of Chinese cabbage using medium containing ethylene inhibitor AgNO₃ [J]. Acta Hortic. Sin., 1997, 24(1): 94-96.
[20]	姜迎春.绿色植物生长调节剂(GGR)在农业上的推广应用[J].黑龙江生态工程职业学院学报,2008,21(1):41+122.
	JIANG Y C. Popularization and application of green plant growth regulator (GGR) in agriculture[J]. J. Heilongjiang Vocat. Inst. Ecol. Eng., 2008, 21(1): 41+122.
[21]	周乾,王可,樊芬,等.生根粉在大田作物上的应用效果[J].作物研究,2014,28(4):447-450.
	ZHOU Q, WANG K, FAN F, et al.. Application effect of rooting powder on field crops[J]. Crop Res., 2014, 28(4): 447-450.

消毒时间/min	接种个数	培养时间/d	发芽率/%	污染率/%	死亡率/%	无菌外植体获得率/%
10	40	20	98.31±0.68a	23.73±9.63a	1.69±0.64b	74.58±3.93a
12	40	20	94.36±0.76a	3.47±0.71b	5.64±0.75a	90.89±6.73a
15	40	20	88.99±0.87a	3.13±0.89b	11.01±0.86a	86.90±7.30a
18	40	20	76.83±3.84a	7.68±1.07b	10.97±3.84a	84.68±8.51a

消毒时间/min	接种个数	培养时间/d	发芽率/%	污染率/%	死亡率/%	无菌外植体获得率/%
10	40	20	98.31±0.68a	23.73±9.63a	1.69±0.64b	74.58±3.93a
12	40	20	94.36±0.76a	3.47±0.71b	5.64±0.75a	90.89±6.73a
15	40	20	88.99±0.87a	3.13±0.89b	11.01±0.86a	86.90±7.30a
18	40	20	76.83±3.84a	7.68±1.07b	10.97±3.84a	84.68±8.51a

处理	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	1.0	0.2	40	30	87.5±9.01bc
2	1.5	0.4	40	30	99.2±7.64b
3	2.0	0.6	40	30	93.3±12.6b
4	1.0	0.4	40	30	80.0±9.01c
5	1.5	0.6	40	30	86.7±1.44bc
6	2.0	0.2	40	30	105.8±15.3a
7	1.0	0.6	40	30	58.3±21.8d
8	1.5	0.2	40	30	113.3±11.8a
9	2.0	0.4	40	30	91.7±6.3b

处理	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	1.0	0.2	40	30	87.5±9.01bc
2	1.5	0.4	40	30	99.2±7.64b
3	2.0	0.6	40	30	93.3±12.6b
4	1.0	0.4	40	30	80.0±9.01c
5	1.5	0.6	40	30	86.7±1.44bc
6	2.0	0.2	40	30	105.8±15.3a
7	1.0	0.6	40	30	58.3±21.8d
8	1.5	0.2	40	30	113.3±11.8a
9	2.0	0.4	40	30	91.7±6.3b

处理	AgNO₃/(mg·L^-1)	KT/(mg·L^-1)	NAA/(mg·L^-1)	接种个数	培养时间/d	增殖率/%
1	0	1.0	0.2	40	30	46.67±23.63b
2	0	1.5	0.4	40	30	47.50±10.00b
3	0	2.0	0.6	40	30	40.38±7.63bc
4	0.8	1.0	0.4	40	30	51.67±12.83ab
5	0.8	1.5	0.6	40	30	35.00±17.32bc
6	0.8	2.0	0.2	40	30	71.67±5.77a
7	1.6	1.0	0.6	40	30	30.83±5.20b
8	1.6	1.5	0.2	40	30	58.33±7.22a
9	1.6	2.0	0.4	40	30	38.33±8.78b

Optimization of Rapid Propagation System of Chinese Herbal Medicine Reynoutria japonica Houtt. in Vitro

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 21

Related Articles 1

Recommended Articles

Metrics

Comments

激素处理	接种个数	生根率/%	移栽成活率/%	观察现象
IBA	45	51.11	100	生根培养30 d，根长且粗壮，但出根慢，培养时间长
GGR6号	45	51.11	100	生根培养15 d，根长，多为3根左右，培养3 d可以观察到出根点

GGR6号/(mg·L^-1)	接种个数	培养时间/d	生根率/%	移栽成活率/%
3.0	20	15	70.37±16.97a	100±0.00
4.0	20	15	66.67±11.11ab	100±0.00
5.0	20	15	59.26±12.83ab	100±0.00
6.0	20	15	44.44±11.11b	100±0.00
7.0	20	15	59.26±6.42ab	100±0.00