Abstract:

By analyzing the diversity of GH10 and GH11 genes in the rumen of domestic sheep from the Pamir Plateau in Xinjiang， this study provides new genetic resources for elucidating the enzymatic properties of GH10 and GH11 family xylanases， while also laying a foundation for understanding the structure of rumen xylanases. This study collected rumen contents from six domestic sheep on the Pamir Plateau in Xinjiang. Total genomic DNA was extracted， and universal primers specific to each family were used for amplification. High-throughput sequencing was performed， and bioinformatics methods were applied to analyze the sequencing results. Following quality control， operational taxonomic unit （OTU） clustering analysis identified that the GH10 gene family contained a total of 2 040 operational taxonomic units （OTUs）， with 18 OTUs showing relative abundance greater than 0.01； the GH11 gene family contained a total of 825 OTUs， with 14 OTUs having a relative abundance greater than 0.01. NCBI homology comparisons revealed that GH10 sequences were classified into 4 phyla， 5 families and 6 genera， with 97.79% of counts remaining unclassified， while GH11 sequences were assigned to 4 phyla， 8 families and 10 genera， with 69.94% of counts unclassified. Alpha diversity analysis demonstrated that GH10 exhibited higher diversity， evenness and richness indices compared to GH11. The rumen of domestic sheep in the Xinjiang Pamir Plateau harbors abundant GH10 and GH11 family xylanases， which exhibit certain specificity and the GH10 gene family displays higher diversity than the GH11 gene family.

Key words: domestic sheep, Pamir Plateau, GH10 family, GH11 family, xylanases, rumen