Abstract:

The study aims to investigate the alleviating effect of Hylocereus undatus on the intestinal damage in fruit flies， and explore the possible mechanism， with the goal of providing theoretical basis for the development and application of H. undatus. An acute intestinal injury model of wild-type Drosophila melanogaster was induced by dextran sulfate sodium salt （DSS）. The effects of feeding normal medium and medium containing different concentrations （0.5%， 1%， 5%） of H. undatus on the survival indicators （locomotor ability， activity and survival rate）， antioxidant indicators （superoxide dismutase， catalase， reduced glutathione， malondialdehyde）， and intestinal injury conditions （smurf assay， lactate dehydrogenase， Ca²⁺ content determination） of Drosophila melanogaster were analyzed. The results showed that the acute intestinal injury model of Drosophila melanogaster was successfully constructed by DSS. After feeding with medium containing H. undatus， the survival rate， locomotor ability and activity of Drosophila melanogaster were significantly improved， the activities of superoxide dismutase， catalase and lactate dehydrogenase and the content of reduced glutathione increased， while the contents of Ca²⁺ and malondialdehyde decreased， and the intestinal injury was significantly alleviated. Moreover， the high concentration group （containing 5% H. undatus） had the most significant effect. H. undatus has an alleviating effect on the intestinal injury induced by DSS in Drosophila melanogaster， which may be related to the repair of intestinal cell damage and the enhancement of antioxidant capacity by H. undatus.

Key words: Hylocereus undatus, Drosophila melanogaster, intestinal damage, anti-oxidant