Abstract: Rhizopus arrhizus was applied in fumaric acid fermentation with unhydrolyzed raw tapioca flour as the carbon source. The effects of tapioca flour fineness, the calcium carbonate amount, oxygen vectors additive and rotation speed in flasks on fumaric acid prodution from raw tapioca flour by Rhizopus arrhizus was investigated. The fermentation processes of glucose and raw tapioca flour as two carbon sources in flasks were compared. A maximum fumaric acid production of 46.36 g/L in flasks was obtained with a productivity of 0.32 g/L·h. Using a selfmade 2.5 L airlift reactor(culture temperature 30℃, aeration rate 154 vvm, the filling volume 1.3 L), the fumaric acid production was up to 42.32 g/L, with a productivity of 0.29 g/L·h.

Key words: Rhizopus arrhizus, fumaric acid, raw tapioca flour, simultaneous saccharification, airlift reactor

摘要： 以生木薯粉作为碳源,利用少根根霉发酵生产富马酸。考察了木薯粉细度、碳酸钙添加量、转速及添加氧载体对富马酸产量的影响。在摇瓶发酵情况下,比较了葡萄糖和生木薯粉两种碳源生产富马酸发酵过程。摇瓶发酵时,富马酸的产量最高可达46.36 g/L,时空产率达0.32 g/L/h。采用2.5 L自制气升式发酵罐(培养温度30℃、通气量1.54 vvm、装液量1.3 L),发酵培养144 h后,富马酸产量达42.32 g/L,时空产率达0.29 g/L/h。

关键词: 少根根霉, 富马酸, 生木薯粉, 同步糖化, 气升式反应器