Advance in Genetic Modification Tools and High-value Compound Production in Methanogenic Archaea

doi:10.19586/j.2095-2341.2026.0108

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (3): 485-495.DOI: 10.19586/j.2095-2341.2026.0108

• Special Forum on Microbial Cell Factory • Previous Articles Next Articles

Advance in Genetic Modification Tools and High-value Compound Production in Methanogenic Archaea

Xiaoxuan WU¹(), Yuqian LIANG¹, Tianer ZHENG¹^,², Boyan LIU¹, Lei CHENG¹^,³, Yan HUANG¹()

^1.Key Laboratory of Development and Application of Rural Renewable Energy，Biogas Institute of Ministry of Agriculture and Rural Affairs，Chengdu 610041，China
^2.Gembloux Agro Bio Tech，University of Liège，Gembloux 5030，Belgium
^3.Shenzhen Branch，Guangdong Laboratory of Lingnan Modern Agriculture，Key Laboratory of Synthetic Biology，Ministry of Agriculture and Rural Affairs，Agricultural Genomics Institute at Shenzhen，Chinese Academy of Agricultural Sciences，Guangdong Shenzhen 518124，China

Received:2026-04-08 Accepted:2026-04-20 Online:2026-05-25 Published:2026-07-14
Contact: Yan HUANG

Abstract

Abstract:

Methanogenic archaea are strictly anaerobic microorganisms capable of utilizing one-carbon （C₁） or two-carbon （C₂） compounds for growth and methane production. Owing to their unique membrane lipid structures and tolerance to various extreme environmental conditions， they represent promising chassis cells for the high-value conversion of carbon resources such as CO₂. To date， genetic engineering toolkits have been progressively established in model methanogens， particularly Methanosarcina and Methanococcus， including shuttle vectors， expression regulation systems， homologous recombination， and gene editing technologies. Leveraging these tools， methanogenic archaea have been metabolically engineered to produce a range of high-value non-methane compounds， including isoprenoids and bioplastics， as well as metabolic intermediates such as organic acids and organic alcohols， through metabolic pathway remodeling and the integration of heterologous metabolic pathways. This review systematically summarized the research progress of genetic engineering tools and high-value compound synthesis in methanogenic archaea， evaluated the application potential of methanogenic archaeal cell factories， and outlined future research directions， which was expected to provide reference for advance synthetic biology research in methanogens.

Key words: methanogens, genetic engineering, synthetic biology, carbon resource utilization

CLC Number:

Q939

Xiaoxuan WU, Yuqian LIANG, Tianer ZHENG, Boyan LIU, Lei CHENG, Yan HUANG. Advance in Genetic Modification Tools and High-value Compound Production in Methanogenic Archaea[J]. Current Biotechnology, 2026, 16(3): 485-495.

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Figures/Tables 2

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菌株	底物	策略	产物	产量	参考文献
M. maripaludis	CO₂	表达crtB、crtI基因；优化MVA途径	番茄红素	未报道	[76]
M. maripaludis	H₂/CO₂或甲酸盐	表达罗勒来源的香叶醇合酶基因(ges)	香叶醇	2.8 mg·g^-1细胞干重(H₂/CO₂）； 4.0 mg·g^-1细胞干重（甲酸盐）； 4.6 mg·g^-1细胞干重（甲酸盐；无选择压力）	[50]
M. acetivorans	甲醇、三甲胺、乙酸	表达杨树来源的异戊二烯合酶基因(ispS)	异戊二烯	855 μmol·L^-1	[49]
M. acetivorans	市政废水	表达杨树来源的异戊二烯合酶基因(ispS)	异戊二烯	0.97 mmol·L^-1	[70]

菌株	底物	策略	产物	产量	参考文献
M. maripaludis	CO₂	表达crtB、crtI基因；优化MVA途径	番茄红素	未报道	[76]
M. maripaludis	H₂/CO₂或甲酸盐	表达罗勒来源的香叶醇合酶基因(ges)	香叶醇	2.8 mg·g^-1细胞干重(H₂/CO₂）； 4.0 mg·g^-1细胞干重（甲酸盐）； 4.6 mg·g^-1细胞干重（甲酸盐；无选择压力）	[50]
M. acetivorans	甲醇、三甲胺、乙酸	表达杨树来源的异戊二烯合酶基因(ispS)	异戊二烯	855 μmol·L^-1	[49]
M. acetivorans	市政废水	表达杨树来源的异戊二烯合酶基因(ispS)	异戊二烯	0.97 mmol·L^-1	[70]

Advance in Genetic Modification Tools and High-value Compound Production in Methanogenic Archaea

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