Food safety is a major strategic issue related to the national economy and people's livelihood. Pesticide residue is an important factor. Pesticides residues in foods not only occur at a high rate， but also exceed the standard severely and involve a wide range. Long-term exposure or consumption of food containing pesticide residues can endanger human health. Traditional techniques for analyzing pesticides in foods have been difficult to meet the actual needs of diversified food safety tests， and modern novel biotechnology has been widely used and continuously developed in the detection of pesticide residues in food due to the advantages of high specificity， high sensitivity and accurate results. This paper analyzed the current situation of pesticide contamination and the corresponding detection technologies in foods， then reviewed the application progress of immunoassay techniques， such as enzyme-linked immunosorbent assay， fluorescence immunoassay and biobarcode immunoassay， and biosensing techniques including electrochemical， optical and piezoelectric sensors in the detection of pesticide residues in food， and discussed the advantages and limitations of various techniques with a view to providing relevant research. The advantages and limitations of various techniques were discussed with the aim of providing references for relevant research， improving the quality and efficiency of pesticide residue detection in food in China， making it more useful and valuable for people's dietary safety， and promoting the sustainable development of China's food industry.

Plasticizers， as a processing aid， which can increase the plasticity of polymers， have been widely used in chemical， pharmaceutical， daily necessities， food packaging and other fields. Phthalates are the most used plasticizers， which are found to migrate into food during production and distribution， causing irreversible damage to the human body. This article reviewed the principles， advantages and disadvantages and applicability of pretreatment methods and traditional detection methods for the detection of phthalates plasticizers in food. Then， according to the different types of output signals， the principles， characteristics， and applications of several phthalate rapid detection methods were reviewed， and their development directions were prospected. Finally， the disadvantages of several rapid detection methods were summarized and compared， with a view to provide reference for the research of plasticizer detection methods， and provide ideas for the development of new rapid detection methods.

The chloropropanol esters were internationally concerned as pollutants in food processing， its hydrolysates （chloropropanols） has a great risk of harm to human body （especially infants）. Therefore， the solid-phase extraction and gas chromatography-mass spectrometry （GC-MS） was used to determining 3-monochloropropane-1，2-diol ester （3-MCPD ester） and 2-monochloropropane-1，3-diol ester （2-MCPD ester） in edible vegetable oil and fatty food. The method had good accuracy （the average recoveries of 3-MCPD ester and 2-MCPD ester were 98.9% and 96.5%， respectively） and sensitivity （the detection limits for 3-MCPD ester and 2-MCPD ester were 0.042 and 0.058 mg·kg^-1， respectively）. Repeatability and reproducibility were satisfactory （the relative standard deviation was below 5%） for all analytes. The concentration of 3-MCPD ester and 2-MCPD ester in 76 batches of monitored samples were in the range of 0.042~4.865 mg·kg^-1 （the average was 0.773 mg·kg^-1） and 0.058~2.592 mg·kg^-1 （the average was 0.469 mg·kg^-1） respectively. Verified by institutions co-verification and proficiency testing of Food Analysis Performance Assessment Scheme （FAPAS） samples， the two chloropropanol esters showed good linearity in the range of 0.200~3.000 mg·kg^-1. The results layed a foundation for further research and established a feasible detection method for food processing enterprises to control the manufacturing process strictly.

Diseases caused by food-borne pathogenic bacteria pose a great threat to human health. Although some pathogenic bacteria such as Staphylococcus aureus， Escherichia coli and Salmonella enterica have made great progress in diagnosis and prevention， it is still a challenge to develop fast， efficient and low-cost detection methods. Functional nucleic acids （FNAs） are a kind of nucleic acids with functions beyond the conventional genetic function of nucleic acids， mainly including natural ribozymes， riboswitches， as well as aptamers， RNAzymes and deoxyribozymes screened by exponential enrichment ligand system evolution （SELEX） in vitro. Because of its high stability， specificity and designability， aptamers and DNAzymes have become an ideal tool for identifying pathogenic microorganisms， which has attracted much attention in the fields of biosensing and medical diagnosis in recent years. This paper reviewed the screening principle and process of functional nucleic acids， the application progress and challenges of aptamers and RNA cleavage deoxyribozymes （RCDs） in the detection of pathogenic bacteria， and prospected their future development prospects.