Abstract: The culture-independent technique of 16S rRNA clone and sequencing in samples of environments suggests that there exists a host of unique and culture-independent microbes in nature. It is an important subject to learn how to explore as many as microbes. In view of that, this paper reviews the mainly reasons why is difficult for us to culture these culture-independent microbes and introduces the innovative culture technique and the improved culture medium those are proved stunning effective recently. By doing that we believe more and more innovative culture technique will show up in near future.

Key words: culture-independent microbes, culture technique, new drug, microcosm