Abstract: A liquid chromatographic method for determination of phosphatidylcholine in Zophobsmorio L. has been developed. The samples were analyzed by liquid chromatography. Quantitative method was external standard method. The linearity was checked by standard solutions at concentration range from 250～2 000 μg/mL, the correlation coefficient was 0.999 6. The recovery values ranged from 97% to 109% with relative standard deviations(RSDs) no larger than 2.4%. For different growth stage of Zophobsmorio L., the results of the content of phosphatidylcholine in vivo showed that the content of phosphatidylcholine was in line with the binomial curve along with the change of the weight, the correlation coefficient R2 was 0.865 (P<0.01).

Key words: liquid chromatography, phosphatidylcholine, Zophobsmorio L.