Research on Fourth-generation Sequencing Based on Biological Nanopores

doi:10.19586/j.2095-2341.2025.0148

Current Biotechnology ›› 2026, Vol. 16 ›› Issue (4): 898-908.DOI: 10.19586/j.2095-2341.2025.0148

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Research on Fourth-generation Sequencing Based on Biological Nanopores

Xinxin ZHANG(), Tianli GUO, Hua GUAN()

The First Research Institute of the Ministry of Public Security of the People's Republic of China，Beijing 100044，China

Received:2025-10-29 Accepted:2026-01-21 Online:2026-07-25 Published:2026-09-11
Contact: Hua GUAN

Abstract

Abstract:

As a crucial pillar of molecular biology， sequencing technology is experiencing rapid development worldwide. Nanopore sequencing technology has emerged as the leading fourth-generation sequencing technology， addressing the limitations of previous-generation technologies in terms of throughput， read length， portability and cost. By comparing the key parameters of traditional sequencing and nanopore sequencing， this study elaborated on the principles of biological nanopore sequencing and the composition of sequencing instruments， analyzed the structural characteristics and functional adaptability of biological nanopore proteins， expounded on the application scenarios of nanopore sequencing in genomics， transcriptomics， epigenetics and other fields， and prospected for the development of sequencing technology. This paper aims to provide a comprehensive reference for researchers to deeply understand nanopore sequencing technology， and facilitates the further promotion， optimization and upgrading of this technology in life science research and clinical practice.

Key words: nanopore sequencing principle, nanopore protein, genomics, transcriptomics, epigenetics

CLC Number:

Q-337

Xinxin ZHANG, Tianli GUO, Hua GUAN. Research on Fourth-generation Sequencing Based on Biological Nanopores[J]. Current Biotechnology, 2026, 16(4): 898-908.

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URL: https://swjsjz.magtechjournal.com/EN/10.19586/j.2095-2341.2025.0148

https://swjsjz.magtechjournal.com/EN/Y2026/V16/I4/898

Figures/Tables 3

References 69

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Research on Fourth-generation Sequencing Based on Biological Nanopores

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