Research Progress of Cyanobacteria Cell Factories

doi:10.19586/j.2095-2341.2022.0203

Current Biotechnology ›› 2023, Vol. 13 ›› Issue (2): 174-180.DOI: 10.19586/j.2095-2341.2022.0203

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Research Progress of Cyanobacteria Cell Factories

Junkui ZHAO(), Yongzhong LU()

College of Marine Science and Biological Engineering，Qingdao University of Science and Technology，Shandong Qingdao 266042，China

Received:2022-11-03 Accepted:2023-01-06 Online:2023-03-25 Published:2023-04-07
Contact: Yongzhong LU

Abstract

Abstract:

With strong carbon fixation capacity and amenability to genetic manipulations， cyanobacteria are considered to be ideal cell factories for developing green biotechnology products. However， many research achievements are still in the laboratory stage， and the production efficiency of various chemicals is low for a long time. Combining the advantages of cyanobacteria and making full use of modern biotechnology will help promote the in-depth research and development of cyanobacterial cell factories. Taking the development process of cyanobacterial biotechnology as the main line， this paper introduced the achievements of genetic engineering， metabolic engineering and synthetic biology in the research of cyanobacterial cell factory， and pointed out that "drug and food homology" was the direction of foreign protein expression by cyanobacteria for application. To develop advanced synthetic biological tools so as to transform， design and reconstruct the metabolic pathways of cyanobacteria chassis cells was the only way to achieve efficient production of chemicals. This paper was expected to provide a reference for the in-depth study of cyanobacterial biotechnology.

Key words: cyanobacteria, genetic engineering, metabolic process, chemical, chassis cells, synthetic biology

CLC Number:

Junkui ZHAO, Yongzhong LU. Research Progress of Cyanobacteria Cell Factories[J]. Current Biotechnology, 2023, 13(2): 174-180.

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Figures/Tables 2

References 55

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外源蛋白	功能	藻株	文献
人肝金属硫蛋白-I_A（hMT-I_A）	金属代谢、抗氧化等	Anabaena PCC7120	[6]
人尿激酶原基因(pro-urokinase)	溶栓	Synechococcus sp. PCC7002	[7]
人肿瘤坏死因子α（hTNF-α）	杀伤肿瘤细胞	Anabaena PCC7102	[8]
人表皮生长因子（hEGF）	促进表皮细胞生长等	Synechococcus sp. PCC7002	[9]
人铜锌超氧化物歧化酶（hCu，Zn-SOD）	抗氧化、抗辐射损伤等	Synechococcus sp. PCC7942	[10]
人粒-巨噬细胞集落刺激因子（hGM-CSF）)	造血调控、免疫调节等	Anabaena PCC7102	[11]
Eta1-L-Gapdh蛋白	预防鱼类迟缓爱德华氏菌感染	Anabaena PCC7120	[12]
病毒包膜蛋白（vp28）	预防对虾白斑综合征病毒	Synechococcus sp. PCC7942, Anabaena PCC7120	[13-15]
人粒细胞集落刺激因子（hG-CSF）	造血调控	Anabaena PCC7102	[16]
病毒包膜蛋白（vp19）	预防对虾白斑综合征病毒	Synechococcus sp. PCC7942	[17]
胸腺α₁（Tα₁）	增强免疫	Spirulina	[18]

外源蛋白	功能	藻株	文献
人肝金属硫蛋白-I_A（hMT-I_A）	金属代谢、抗氧化等	Anabaena PCC7120	[6]
人尿激酶原基因(pro-urokinase)	溶栓	Synechococcus sp. PCC7002	[7]
人肿瘤坏死因子α（hTNF-α）	杀伤肿瘤细胞	Anabaena PCC7102	[8]
人表皮生长因子（hEGF）	促进表皮细胞生长等	Synechococcus sp. PCC7002	[9]
人铜锌超氧化物歧化酶（hCu，Zn-SOD）	抗氧化、抗辐射损伤等	Synechococcus sp. PCC7942	[10]
人粒-巨噬细胞集落刺激因子（hGM-CSF）)	造血调控、免疫调节等	Anabaena PCC7102	[11]
Eta1-L-Gapdh蛋白	预防鱼类迟缓爱德华氏菌感染	Anabaena PCC7120	[12]
病毒包膜蛋白（vp28）	预防对虾白斑综合征病毒	Synechococcus sp. PCC7942, Anabaena PCC7120	[13-15]
人粒细胞集落刺激因子（hG-CSF）	造血调控	Anabaena PCC7102	[16]
病毒包膜蛋白（vp19）	预防对虾白斑综合征病毒	Synechococcus sp. PCC7942	[17]
胸腺α₁（Tα₁）	增强免疫	Spirulina	[18]

产物	功能	藻种名	文献
色氨酸	动物饲料添加剂等	Synechocystis sp. PCC6803	[21]
二十碳五烯酸	降血脂、降血压等	Synechococcus sp. NKBG042902	[22]
ω-3脂肪酸	调整血脂、生物能源	Synechococcus sp. NKBG15041c	[23]
柠檬烯	镇咳、祛痰、抑菌等	Synechocystis sp. PCC6803	[24]
鲨烯	药用	Synechocystis sp. PCC6803	[25]
肌醇	生长因子	Synechocystis sp. PCC6803	[26]
虾青素	抗氧化	Synechocystis sp. PCC6803	[27]
维生素B2	维生素	Synechococcus sp. PCC7002	[28]
木糖醇	防龋齿、改善肝功能、调节肠道等	Synechococcus sp. PCC7942	[29]
海澡糖	食品添加剂	Synechocystis sp. PCC6803	[30]
乙醇	生物能源	Synechococcus sp. PCC7942, Synechococcus sp. PCC7002	[31]
氢气	生物能源	Cyanothece sp. ATCC51142	[32]
聚羟基脂肪酸酯	生物塑料	Synechococcus sp. MA19	[32]
乙烯	化工原料	Synechocystis sp. PCC6803	[33]
异丁醇	化工原料、能源	Synechocystis sp. PCC6803	[34]
丁二醇	化工原料、能源	Synechococcus sp. PCC7942	[35]
聚乳酸	生物塑料	Synechococcus sp. PCC7942	[36]

产物	功能	藻种名	文献
色氨酸	动物饲料添加剂等	Synechocystis sp. PCC6803	[21]
二十碳五烯酸	降血脂、降血压等	Synechococcus sp. NKBG042902	[22]
ω-3脂肪酸	调整血脂、生物能源	Synechococcus sp. NKBG15041c	[23]
柠檬烯	镇咳、祛痰、抑菌等	Synechocystis sp. PCC6803	[24]
鲨烯	药用	Synechocystis sp. PCC6803	[25]
肌醇	生长因子	Synechocystis sp. PCC6803	[26]
虾青素	抗氧化	Synechocystis sp. PCC6803	[27]
维生素B2	维生素	Synechococcus sp. PCC7002	[28]
木糖醇	防龋齿、改善肝功能、调节肠道等	Synechococcus sp. PCC7942	[29]
海澡糖	食品添加剂	Synechocystis sp. PCC6803	[30]
乙醇	生物能源	Synechococcus sp. PCC7942, Synechococcus sp. PCC7002	[31]
氢气	生物能源	Cyanothece sp. ATCC51142	[32]
聚羟基脂肪酸酯	生物塑料	Synechococcus sp. MA19	[32]
乙烯	化工原料	Synechocystis sp. PCC6803	[33]
异丁醇	化工原料、能源	Synechocystis sp. PCC6803	[34]
丁二醇	化工原料、能源	Synechococcus sp. PCC7942	[35]
聚乳酸	生物塑料	Synechococcus sp. PCC7942	[36]

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