Abstract:

With the rapid development of nucleic acid detection kit in the field of rapid microbial detection， the detection process has the advantages of simple operation， low cost and convenience for on-site detection. In the preparation process of nucleic acid detection kit for food-borne pathogenic bacteria， it is necessary to ensure its qualitative and quantitative measurement ability through metrological traceability. The application of nucleic acid reference materials （NARMs） runs through many fields， such as clinical hygiene， food， instrument verification/calibration， nucleic acid detection， etc. By segmenting genetic materials， the biological safety can be guaranteed to the greatest extent， and the requirements of quality evaluation and metrological traceability can be met. It can effectively control the quality of nucleic acid detection kits and provide an accurate “yardstick” for the quantitative evaluation of nucleic acid detection kits. This paper introduced the development method of NARMs in China， focusing on the current research status of food-borne pathogens and its application in the national standard material resource sharing platform， and analyzed the technical challenges and the possibility of the development of nucleic acid metrology in the future， so as to provide reference ideas for the early diagnosis and prevention of nucleic acid detection industry.

Key words: nucleic acid reference materials, foodborne pathogenic bacteria, in vitro diagnosis, food safety